{"id":13305,"date":"2017-08-28T11:18:24","date_gmt":"2017-08-28T03:18:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13305"},"modified":"2022-07-21T11:12:37","modified_gmt":"2022-07-21T03:12:37","slug":"%e9%88%a3%e8%aa%bf%e8%9b%8b%e7%99%bd%e7%93%8a%e8%84%82%e7%b3%96%e5%87%9d%e8%86%a0-calmodulin-sepharose-beads-%e8%b2%a8%e8%99%9f7934","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13305","title":{"rendered":"\u9223\u8abf\u86cb\u767d\u74ca\u8102\u7cd6\u51dd\u81a0 | Calmodulin-Sepharose Beads \u8ca8\u865f7934"},"content":{"rendered":"

\u86cb\u767d\u8cea\u7d14\u5316\u6a39\u8102<\/strong>\u7cfb\u5217\u5546\u54c1<\/strong><\/span><\/p>\n

\u91cd\u7d44\u86cb\u767d\u7684\u8868\u9054\u548c\u7d14\u5316\u5c0d\u65bc\u7814\u7a76protein regulation\u86cb\u767d\u8cea\u8abf\u7bc0\uff0cStructure\u7d50\u69cb\u53cafunction\u529f\u80fd\u7814\u7a76\u70ba\u91cd\u8981\u7814\u7a76\u5de5\u5177\u3002\u5927\u591a\u6578\u91cd\u7d44\u86cb\u767d\u8cea\u8868\u9054\u70ba\u5177\u6709\u77ed\u89aa\u548c\u529b\u7684\u878d\u5408\u86cb\u767d\u6a19\u7c64\u6216\u5c0f\u86cb\u767d\u8cea\uff0c\u5982GST\u3002 GST\u70ba\u7279\u7570\u6027\u7d50\u5408\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u3002 BioVision\u7684GlutathioneSepharose\u00ae\u901a\u904e\u5171\u50f9\u5076\u806fglutathione\u7a40\u80f1\u7518\u80bd\u3001\u8207epoxy-activated\u74b0\u6c27\u6d3b\u5316\u76844\uff05cross-linked sepharose beads\u4ea4\u806f\u74ca\u8102\u7cd6\u51dd\u81a0\uff0c\u4f86\u88fd\u5099\u5f62\u6210\u7a69\u5b9a\u7684\u786b\u919a\u9375thioether linkage\u3002Heparin-Sepharose\u00ae\u5ee3\u6cdb\u7528\u65bc\u7d14\u5316\u8840\u6e05\u86cb\u767d\u5305\u62eccoagulation factors\u51dd\u8840\u56e0\u5b50\u3001lipases\u8102\u80aa\u9176\u3001lipoproteins\u8102\u86cb\u767d\uff0chormone receptors\u6fc0\u7d20\u53d7\u9ad4\u548cgrowth factors\u751f\u9577\u56e0\u5b50\u3002 BioVision\u516c\u53f8\u7684Heparin-Sepharose\u00ae\u901a\u904e\u5171\u50f9\u9023\u63a5\u809d\u7d20\u8207\u74b0\u6c27\u81ea\u7531\u57fa6\uff05\u4ea4\u806f\u800c\u88fd\u5099\u74ca\u8102\u7cd6\u73e0\u3002\u512a\u5316\u4e86\u7a40\u80f1\u7518\u80bd\u548c\u809d\u7d20 – \u74ca\u8102\u7cd6\u51dd\u81a0\u7684\u5076\u806f\u6280\u8853\u9ad8\u7d50\u5408\u80fd\u529b\uff08\u22655 mg GST \/ ml Glutathione-Sepharose resin ; Heparin-Sepharose\u00ae resin\u22650.4mg\u809d\u7d20\u7d50\u5408\u86cb\u767d\/ ml\uff09\uff0c\u5177\u9ad8\u6d41\u901f\u548c\u6700\u5c0f\u6d78\u51fa\u914d\u9ad4\u3002<\/p>\n

Calmodulin-Sepharose Beads <\/strong>\u9223\u8abf\u86cb\u767d\u74ca\u8102\u7cd6\u51dd\u81a0 | <\/strong>\u8ca8\u865f7934<\/strong><\/a><\/span><\/p>\n

Ideal for the specific enrichment of calmodulin-binding proteins from biological samples.<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Calmodulin\u9223\u8abf\u86cb\u767d\u662f\u53c3\u8207\u5404\u7a2e\u7d30\u80de\u904e\u7a0b\u7684\u9ad8\u5ea6\u4fdd\u5b88\u7684\u8abf\u63a7\u771f\u6838\u86cb\u767d\uff0c\u4f8b\u5982glycogen metabolism\u7cd6\u539f\u4ee3\u8b1d\uff0c, cytoskeletal control\u7d30\u80de\u9aa8\u67b6\u63a7\u5236\uff0cneurotransmission\u795e\u7d93\u50b3\u905e\uff0cphosphate activity\u78f7\u9178\u6d3b\u6027\u548cNAD \/ NADP\u7684\u63a7\u5236\u3002 \u9223\u8abf\u86cb\u767d\u7684\u591a\u6a23\u529f\u80fd\u4f9d\u8cf4\u65bc\u5176\u8207\u5927\u91cf\u9776\u86cb\u767d\/\u9176\u7684\u76f8\u4e92\u4f5c\u7528\u548c\u8abf\u63a7\u5176\u6d3b\u6027\u7684\u80fd\u529b\u3002 \u9223\u8abf\u86cb\u767d\u901a\u5e38\u901a\u904e\u5176\u758f\u6c34\u90e8\u4f4d\u7d50\u5408\u86cb\u767d\u8cea\uff0c\u5176\u7531\u65bcCa 2+\u5b58\u5728\u4e0b\u7684\u69cb\u8c61\u8b8a\u5316\u800c\u66b4\u9732\u3002 \u7136\u800c\uff0c\u4e00\u4e9b\u76ee\u6a19\u86cb\u767d\u4ee5\u9223\u4f9d\u8cf4\u7684\u65b9\u5f0f\u8207\u9223\u8abf\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u3002Calmodulin-Sepharose beads\u5141\u8a31\u5f9e\u751f\u7269\u6a23\u54c1\u4e2d\u7279\u7570\u6027\u5bcc\u96c6\u5df2\u77e5\u6216\u672a\u77e5\u7684\u9223\u8abf\u86cb\u767d\u7d50\u5408\u86cb\u767d\u3002 \u6b64\u5916Calmodulin-Sepharose beads\u63d0\u4f9b\u4e86\u7528\u65bc\u89aa\u548c\u7d14\u5316\u9223\u8abf\u86cb\u767d\u7d50\u5408\u80bd\uff08calmodulin binding peptide , CBP\uff09\u6a19\u8a18\u7684\u91cd\u7d44\u86cb\u767d\u7684\u4fbf\u5229\u5de5\u5177\u3002 CBP\u6a19\u8a18\u86cb\u767d\u7684Elution\u53ef\u4ee5\u901a\u904e\u7c21\u55ae\u5730\u52a0\u5165chelation ligand\u87af\u5408\u914d\u9ad4\/\u53bb\u9664Ca2 +\u4f86\u5b8c\u6210\u3002<\/p>\n

\u76f8\u95dc\u5546\u54c1<\/span>\u00a0Ni-IDA Spin Columns (His-tag)\u00a0<\/a>\u00a0| \u00a0Ni-IDA\u00a0\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d (\u542bBuffer)\u00a0<\/a>\u00a0|\u00a0Jacalin-<\/a>\u74ca\u8102\u7cd6\u00a0(IgA)\u00a0|\u00a0<\/strong>Calmodulin-Sepharose Beads<\/a>(\u9223\u8abf\u86cb\u767d)\u00a0<\/strong>|\u00a0<\/strong>Albumin-IgG \u53bb\u9664Beads<\/a>\u00a0\u00a0 |\u00a0<\/strong>Cibacron Blue-Agarose Beads<\/a>\u00a0|\u00a0<\/strong><\/strong>\u809d\u7d20\u74ca\u8102\u7cd6<\/a>\u00a0<\/span><\/p>\n\n\n\n\n
Highlights<\/strong><\/td>\nFORMULATION: Supplied as 50% aqueous slurry in 20 % Ethanol.
\nLIGAND DENSITY: ~ 1.5 mg Calmodulin protein per ml of drained resin\/beads.
\nPREPARATION: Calmodulin-Sepharose Beads are prepared by covalently coupling recombinant human calmodulin (Cat # 7838) to 6% cross linked Sepharose beads.
\nAPPLICATIONS:
\n\u2022 Single-step purification of native calmodulin-binding proteins.
\n\u2022 Tandem affinity purification (TAP) of protein complexes.
\n\u2022 Purification of CBP tagged proteins.
\n\u2022 Purification of calmodulin-regulated proteins from all eukaryotic cells.<\/td>\n<\/tr>\n
Highlights<\/strong><\/td>\n\u2022 CONTENTS: Supplied as 50% slurry in 20% Ethanol\/H2O.
\n\u2022 FEATURES: Jacalin-Sepharose beads are prepared by covalently coupling BioVision\u2019s highly purified Jacalin (Cat # 6560) to 6% cross-linked Sepharose beads. The coupling technique is optimized to give a higher binding capacity for IgA & minimal leaching of Jacalin. The binding capacity of Jacalin-Sepharose is \u2265 3 mg human IgA per ml of wet beads.
\n\u2022 APPLICATIONS: Purification of human IgA from serum or colostrum samples or removal of contaminating IgA from IgG samples.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/span> Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u86cb\u767d\u8cea\u7d14\u5316\u6a39\u8102\u7cfb\u5217\u5546\u54c1 \u91cd\u7d44\u86cb\u767d\u7684\u8868\u9054\u548c\u7d14\u5316\u5c0d\u65bc\u7814\u7a76protein regulation\u86cb\u767d\u8cea\u8abf\u7bc0\uff0cStruc […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3283],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13305"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13305"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13305\/revisions"}],"predecessor-version":[{"id":13345,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13305\/revisions\/13345"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13305"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13305"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13305"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}