{"id":13295,"date":"2017-08-27T22:52:28","date_gmt":"2017-08-27T14:52:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13295"},"modified":"2022-07-21T11:12:46","modified_gmt":"2022-07-21T03:12:46","slug":"13295","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13295","title":{"rendered":"Ni-IDA \u74ca\u8102\u7cd6\uff5cNi-IDA Agarose Beads\u8ca8\u865fG250"},"content":{"rendered":"

Ni-IDA Agarose Beads<\/span>\uff5c\u8ca8\u865fG250<\/span><\/a><\/span><\/p>\n

for His-tagged Protein Purification<\/p>\n

\u89aa\u548c\u5c64\u6790 (Affinity chromatography)\u662f\u57fa\u65bc\u9ad8\u5ea6\u7279\u7570\u6027\u5206\u5b50\u76f8\u4e92\u4f5c\u7528\u5206\u96e2biochemical mixtures<\/span>\u7684\u65b9\u6cd5\u3002 Ni-IDA<\/span>\u74ca\u8102\u7cd6\u88ab\u61c9<\/span>\u7528\u65bc\u7d14\u5316\u516d\u500b\u9023\u7e8c\u7d44\u6c28\u9178\u6b98\u57fa (histidine residue)\u7684\u89aa\u548c\u6a19\u7c64\u4e4b<\/span>\u86cb\u767d\u8cea\u3002 histidine-metal<\/span>\u7d44\u6c28\u9178 – <\/span>\u91d1\u5c6c\u96e2\u5b50\u76f8\u4e92\u4f5c\u7528\uff0c<\/span>\u53ef\u5728\u86cb\u767d\u8cea<\/span>\u5929\u7136\u6216\u8b8a\u6027\u689d\u4ef6\u4e0b\uff0c<\/span>\u5c0d\u4f86\u81ea\u4efb\u4f55\u8868\u9054\u7cfb\u7d71\u4e4b<\/span>Histidine<\/span>\u86cb\u767d\u9032\u884c\u4e00\u6b65\u7d14\u5316\u3002 His-Tag<\/span>\u5e8f\u5217\u8207Ni2 +<\/span>\u967d\u96e2\u5b50\u7d50\u5408\uff0c\u5176\u4f7f\u7528\u4e9e\u6c28\u57fa\u4e8c\u4e59\u9178\u57fa\u5718\uff08IDA<\/span>\uff09\u56fa\u5b9a\u5728\u56fa\u9ad4\u652f\u6301\u7269\u4e0a\u3002 \u53bb\u9664\u96dc\u8cea\u4e26<\/span>\u7d14\u5316His<\/span>\u6a19\u8a18\u7684\u86cb\u767d\u8cea\uff0c<\/span>\u6700\u5f8c<\/span>\u53ef\u4ee5\u7528imidazole<\/span>\u54aa\u5511\u6216\u9084\u539f\u7684pH<\/span>\u6eb6\u6db2eluted<\/span>\u3002Beads\u73e0\u5b50\uff08\u5e7e\u4f55\u5f62\u72c0\uff0c\u5c3a\u5bf8\uff09\u00a0Spherical\u7403\u5f62\uff0cStandard\u6a19\u6e96\uff1a~50-150\u03bcm \u30026\uff05\u74ca\u8102\u7cd6\u3002Binding\/ Loading Capacity 20 – 40\u03bcmol Ni2+ \/ ml\u51dd\u81a0 \u3002\u67f1\u6750\u6599\u805a\u4e19\u70ef (Polypropylene) \u548c\u805a\u4e59\u70ef\u73bb\u7483\u6599\u3002\u00a0 \u5132\u5b58\u6eab\u5ea62 – 8\u00b0C<\/span><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4e4b\u7279\u9ede<\/span><\/strong>:<\/strong> <\/span><\/p>\n

\u5f9e\u7c97\u88c2\u89e3\u6db2\u5230> 95<\/span>\uff05\u7d14\u86cb\u767d\u8cea\u7684\u4e00\u6b65\u7c21\u55ae\u7d14\u5316\u65b9\u6848\u3002<\/p>\n

\u5728\u5929\u7136\u6216\u8b8a\u6027\u689d\u4ef6\u4e0b\u9032\u884c\u7d14\u5316\u3002<\/p>\n

\u86cb\u767d\u7d50\u5408\u80fd\u529b\uff1a\u7d04 20-40<\/span>\u03bcmolNi2 + \/ ml<\/span>\u51dd\u81a0\u3002<\/p>\n

\u7d93\u6fdf\u50f9\u503c, <\/span>\u53ef\u7528\u65bc\u9023\u7e8c\u5faa\u74b0\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n
Product Name<\/strong><\/td>\nSize<\/strong><\/td>\nCat. No.<\/strong><\/td>\n<\/tr>\n
Ni-IDA Agarose Resins for Protein Purification<\/td>\n5.0 ml<\/td>\nG250<\/td>\n<\/tr>\n
Ni-IDA Agarose Resins for Protein Purification<\/td>\n25 ml<\/td>\nG251<\/td>\n<\/tr>\n
Ni-IDA Agarose Resins for Protein Purification<\/td>\n100 ml<\/td>\nG253<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/span><\/strong>HOT!! <\/span><\/strong>\u00a0<\/span>\u9ef4\u6f3f\u83ccPCR<\/span><\/span><\/span>\u5075\u6e2c<\/span><\/span><\/span><\/a>\u00a0|<\/strong>\u00a0DAPI<\/span>\u67d3\u6838<\/span><\/span><\/span><\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/span><\/span><\/span><\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/span><\/span><\/span><\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291<\/span><\/span><\/span> \u7b49\u7cfb\u5217\u5546\u54c1<\/span><\/span><\/span><\/a><\/span><\/p>\n

Ni-IDA\u74ca\u8102\u7cd6\u6a39\u8102\u4ee520\uff05\u4e59\u9187\u7684\u61f8\u6d6e\u6db2\u5f62\u5f0f\u63d0\u4f9b\uff0c\u53ef\u4ee5\u7528\u65bc\u6279\u6b21\u6216\u67f1\u7d14\u5316\u3002 Ni-IDA\u74ca\u8102\u7cd6\u67f1\u63a1\u7528\u73fe\u6210\u7684\u5f62\u5f0f\uff0c\u901a\u904e\u91cd\u529b\u6d41\u52d5\u7d14\u5316\u7d44\u6c28\u9178\u6a19\u8a18\u7684\u86cb\u767d\u8cea\u3002<\/span><\/pre>\n
\u76f8\u95dc\u5546\u54c1\u00a0<\/span><\/span><\/strong>Ni-IDA Spin Columns (His-tag)\u00a0<\/a>\u00a0|\u00a0\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d(\u542bBuffer)\u00a0<\/a><\/strong>\u00a0|\u00a0Jacalin-Sepharose<\/a><\/strong>\u00a0(IgA)\u00a0|\u00a0<\/strong>Calmodulin-Sepharose Beads<\/a><\/strong>(<\/strong>\u9223\u8abf\u86cb\u767d)\u00a0<\/strong>|\u00a0Albumin-IgG Depletion Beads<\/a>\u00a0\u00a0<\/strong><\/span><\/p>\n
<\/pre>\n
\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"
Ni-IDA Agarose Beads\uff5c\u8ca8\u865fG250 for His-tagged Protein Puri […]<\/p>\n","protected":false},"author":3,"featured_media":13069,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3285],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13295"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13295"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13295\/revisions"}],"predecessor-version":[{"id":19293,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13295\/revisions\/19293"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/13069"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13295"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}