{"id":13290,"date":"2017-08-27T22:36:08","date_gmt":"2017-08-27T14:36:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13290"},"modified":"2022-07-21T11:12:50","modified_gmt":"2022-07-21T03:12:50","slug":"%e5%bf%ab%e9%80%9f%e7%b4%94%e5%8c%96his-tag%e8%a1%a8%e7%8f%be%e8%9b%8b%e7%99%bd%e5%90%abbuffer-ready-to-use-ni-ida-spin-purification-kit-%e8%b2%a8%e8%99%9fk6567","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13290","title":{"rendered":"\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d(\u542bBuffer) | Ni-IDA Spin Purification Kit \u8ca8\u865fK6567"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13290\" target=\"_blank\"><strong>\u5feb\u901f\u7d14\u5316<\/strong><strong>His-tag<\/strong><strong>\u8868\u73fe\u86cb\u767d<\/strong><strong>(<\/strong><strong>\u542b<\/strong><strong>Buffer) | <\/strong><strong>\u8ca8\u865f<\/strong><strong>K6567<\/strong><\/a><\/span><\/p>\n<p>Kit with Hi-Bind\u2122 Ni QR Agarose Bead columns, &amp; loading, washing &amp; elution buffers for small scale purification of poly-histidine tagged proteins<\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>: <\/strong>BioVision\u7684Ni-IDA Spin Columns\u662f\u5feb\u901f\u5c0f\u91cf\u7d14\u5316poly-histidine-tagged proteins\u591a\u7d44His-tag\u7684\u86cb\u767d\u8cea\u4e4b\u7406\u60f3\u9078\u64c7\u3002\u6bcf\u4e00\u500bcolumns\u9810\u586b\u5145100\u03bcL\u9ad8\u6027\u80fdHi-Bind\u2122Ni QR\u74ca\u8102\u7cd6\u73e0\uff08Cat\u3002\uff036562\uff09\uff0c\u53ef\u4ee5\u9ad8\u6548\u529b\u6355\u7372\u548c\u7d14\u5316Histidine-tag\u8868\u73fe\u86cb\u767d\u3002\u6bcf\u7ba1\u5c0f\u91cf\u7d14\u5316\u53ef\u9ad8\u90542.8\u6beb\u514b\u7684Histidine-tag\u7684\u86cb\u767d\u8cea\u3002\u67f1\u5167\u7684\u6700\u4f73\u5316\u5c0f\u9ad4\u7a4d\u4e4b\u73e0\u5b50, \u5728\u6b63\u5e38\u8868\u9054\u6c34\u5e73\u4e0b\uff0c\u5141\u8a31\u901a\u904e\u66f4\u5f37\u70c8\u7d50\u5408Histidine-tag\u8868\u73fe\u86cb\u767d\uff0c\u6709\u6548\u7f6e\u63db\u975e\u6a19\u8a18\u86cb\u767d\u8cea\u96dc\u8cea\uff0c\u4e26\u63d0\u9ad8\u6700\u7d42\u7522\u7269\u7684\u7d14\u5ea6\u3002\u7d14\u5316\u7684\u7522\u7387\u548c\u7d14\u5ea6\u662f\u86cb\u767d\u8cea\u7279\u7570\u6027\uff0c\u4e26\u4e14\u53d6\u6c7a\u65bc\u5404\u7a2e\u56e0\u7d20\uff0c\u5982\u86cb\u767d\u8868\u9054\u6c34\u5e73\uff0c\u7d50\u69cb\uff0c\u6eb6\u89e3\u5ea6\u7b49\u3002\u586b\u5145\u5728\u6bcf\u500b\u67f1\u4e2d\u7684\u73e0\u5b50\u6bd4Ni-NTA\u5438\u9644\u5291\u578b\u5f0f\u63d0\u4f9b\u9ad8\u905425\uff05\u7684\u5bb9\u91cf\u3002K6567\u8a66\u5291\u76d2\u9084\u5305\u62eccolumn loading\u67f1\u88dd\u8f09\uff0cwash\u6d17\u6ecc\u6db2\u548celution buffers\u6d17\u812b\u7de9\u885d\u6db2\uff0c\u5feb\u901f\u7d14\u5316Histidine-tag\u7684\u86cb\u767d\u8cea\u3002<\/p>\n<p><img decoding=\"async\" class=\"cboxPhoto\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/6\/k6567.jpg\" alt=\"\" \/><\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td>Cat # +Size<\/td>\n<td>K6567-25<\/td>\n<\/tr>\n<tr>\n<td>Size<\/td>\n<td>25 columns<\/td>\n<\/tr>\n<tr>\n<td>Highlights<\/td>\n<td>Salient Features: Binding Capacity: Upto 2.8 mg poly-histidine tagged proteins per column. Column Content: 25 Ni-IDA spin columns, each containing 100 \u00b5l high performance Hi-Bind\u2122 Ni QR Agarose Beads (Cat. #6562). Bead content: A 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol. Applications: \u2022 Ideal for small scale, one \u2013 step purification of poly-histidine tagged recombinant proteins under native conditions. \u2022 Ideal for small scale, one \u2013 step purification of native proteins and peptides that have an affinity for metal ions. \u2022 Screening of expression levels in a HTP manner. \u2022 Applicable for purification under native and denaturing conditions.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d(\u542bBuffer) | \u8ca8\u865fK6567 Kit with Hi-Bind\u2122 Ni [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3286],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13290"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13290"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13290\/revisions"}],"predecessor-version":[{"id":13294,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13290\/revisions\/13294"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13290"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13290"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13290"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}