{"id":13287,"date":"2017-08-27T22:27:07","date_gmt":"2017-08-27T14:27:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13287"},"modified":"2022-07-21T11:12:54","modified_gmt":"2022-07-21T03:12:54","slug":"%e5%bf%ab%e9%80%9f%e7%b4%94%e5%8c%96his-tag%e8%a1%a8%e7%8f%be%e8%9b%8b%e7%99%bd-ni-ida-spin-columns%e8%b2%a8%e8%99%9f6567-25","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13287","title":{"rendered":"\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d | Ni-IDA Spin Columns\u8ca8\u865f6567-25"},"content":{"rendered":"

Ni-IDA Spin Columns <\/strong>\u5feb\u901f\u7d14\u5316<\/strong>His-tag<\/strong>\u8868\u73fe\u86cb\u767d <\/strong>| <\/strong>\u8ca8\u865f<\/strong>6567-25<\/strong><\/a><\/span><\/p>\n

Columns with Hi-Bind\u2122 Ni QR Agarose Beads for small scale purification of poly-histidine tagged proteins<\/p>\n

Ni-IDA Spin Columns<\/strong>\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>BioVision\u7684Ni-IDA Spin Columns\u662f\u5feb\u901f\u5c0f\u91cf\u7d14\u5316\u9019\u4e9bpoly-histidine-tagged proteins\u591a\u7d44His-tag\u7684\u86cb\u767d\u8cea\u4e4b\u7406\u60f3\u9078\u64c7\u3002\u6bcf\u4e00\u500bcolumns\u9810\u586b\u5145100\u03bcL\u9ad8\u6027\u80fdHi-Bind\u2122Ni QR\u74ca\u8102\u7cd6\u73e0\uff08Cat\u3002\uff036562\uff09\uff0c\u53ef\u4ee5\u9ad8\u6548\u529b\u6355\u7372\u548c\u7d14\u5316Histidine-tag\u8868\u73fe\u86cb\u767d\u3002\u6bcf\u7ba1\u5c0f\u91cf\u7d14\u5316\u53ef\u9ad8\u90542.8\u6beb\u514b\u7684Histidine-tag\u7684\u86cb\u767d\u8cea\u3002\u67f1\u5167\u7684\u6700\u4f73\u5316\u5c0f\u9ad4\u7a4d\u4e4b\u73e0\u5b50, \u5728\u6b63\u5e38\u8868\u9054\u6c34\u5e73\u4e0b\uff0c\u5141\u8a31\u901a\u904e\u66f4\u5f37\u70c8\u7d50\u5408Histidine-tag\u8868\u73fe\u86cb\u767d\uff0c\u6709\u6548\u7f6e\u63db\u975e\u6a19\u8a18\u86cb\u767d\u8cea\u96dc\u8cea\uff0c\u4e26\u63d0\u9ad8\u6700\u7d42\u7522\u7269\u7684\u7d14\u5ea6\u3002\u7d14\u5316\u7684\u7522\u7387\u548c\u7d14\u5ea6\u662f\u86cb\u767d\u8cea\u7279\u7570\u6027\uff0c\u4e26\u4e14\u53d6\u6c7a\u65bc\u5404\u7a2e\u56e0\u7d20\uff0c\u5982\u86cb\u767d\u8868\u9054\u6c34\u5e73\uff0c\u7d50\u69cb\uff0c\u6eb6\u89e3\u5ea6\u7b49\u3002\u586b\u5145\u5728\u6bcf\u500b\u67f1\u4e2d\u7684\u73e0\u5b50\u6bd4Ni-NTA\u5438\u9644\u5291\u578b\u5f0f\u63d0\u4f9b\u9ad8\u905425\uff05\u7684\u5bb9\u91cf\u3002<\/p>\n

\"\"<\/p>\n

\u76f8\u95dc\u5546\u54c1\u00a0<\/span><\/span><\/strong>Ni-IDA Spin Columns (His-tag)\u00a0<\/a>\u00a0|\u00a0\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d(\u542bBuffer)\u00a0<\/a><\/strong>\u00a0|\u00a0Jacalin-Sepharose<\/a><\/strong>\u00a0(IgA)\u00a0|\u00a0<\/strong>Calmodulin-Sepharose Beads<\/a><\/strong>(<\/strong>\u9223\u8abf\u86cb\u767d)\u00a0<\/strong>|\u00a0Albumin-IgG Depletion Beads<\/a>\u00a0\u00a0<\/strong><\/span><\/p>\n\n\n\n\n\n
Cat # +Size<\/td>\n6567-25<\/td>\n<\/tr>\n
Size<\/td>\n25 columns<\/td>\n<\/tr>\n
Highlights<\/td>\nSalient Features: Binding Capacity: Upto 2.8 mg poly-histidine tagged proteins per column. Content: 25 Ni-IDA spin columns, each containing 100 \u00b5l high performance Hi-Bind\u2122 Ni QR Agarose Beads (Cat. #6562). Bead content: A 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol. Applications: \u2022 Ideal for small scale, one \u2013 step purification of poly-histidine tagged recombinant proteins under native conditions. \u2022 Ideal for small scale, one \u2013 step purification of native proteins and peptides that have an affinity for metal ions. \u2022 Screening of expression levels in a HTP manner. \u2022 Applicable for purification under native and denaturing conditions.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1\u00a0<\/span><\/span><\/strong>Ni-IDA Spin Columns (His-tag)\u00a0<\/a>\u00a0|\u00a0\u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d(\u542bBuffer)\u00a0<\/a><\/strong>\u00a0|\u00a0Jacalin-Sepharose<\/a><\/strong>\u00a0(IgA)\u00a0|\u00a0<\/strong>Calmodulin-Sepharose Beads<\/a><\/strong>(<\/strong>\u9223\u8abf\u86cb\u767d) <\/strong>|\u00a0Albumin-IgG Depletion Beads<\/a>\u00a0\u00a0<\/strong><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Ni-IDA Spin Columns \u5feb\u901f\u7d14\u5316His-tag\u8868\u73fe\u86cb\u767d | \u8ca8\u865f6567-25 Columns […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3287],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13287"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13287"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13287\/revisions"}],"predecessor-version":[{"id":13320,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13287\/revisions\/13320"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13287"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13287"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}