{"id":13276,"date":"2017-08-27T18:58:35","date_gmt":"2017-08-27T10:58:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13276"},"modified":"2022-07-21T11:13:03","modified_gmt":"2022-07-21T03:13:03","slug":"hmf3a%e4%ba%ba%e4%b9%b3%e8%85%ba%e6%88%90%e7%ba%96%e7%b6%ad%e7%b4%b0%e8%83%9e%ef%bd%9cimmortalized-human-mammary-fibroblasts-hmf3a%e8%b2%a8%e8%99%9ft0153","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13276","title":{"rendered":"HMF3A\u4eba\u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff5cImmortalized Human Mammary Fibroblasts (HMF3A)\u8ca8\u865fT0153"},"content":{"rendered":"

HOT!!<\/b><\/span>\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

\u6c38\u751f\u5316\u4eba\u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff08HMF3A\uff09\uff5c\u8ca8\u865fT0153<\/a><\/span><\/p>\n

Immortalized Human Mammary Fibroblasts (HMF3A)<\/strong><\/p>\n

\u901a\u904e\u8207SV40\u5927T\u6297\u539f\u7684\u6eab\u5ea6\u654f\u611f\u7a81\u8b8a\u9ad4\uff08U19tsA58\uff09, \u548c\u4eba\u7aef\u7c92\u9176\uff08hTERT\uff09\u7684\u50ac\u5316\u4e9e\u55ae\u4f4d\u7d44\u5408, \u8f49\u5c0e\u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\u7522\u751f, \u6c38\u751f\u5316\u4eba\u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff08HMF3A\uff09\u3002 HMF3A\u7d30\u80de\u572833.5\u2103\u7684\u6eab\u5ea6\u4e0b\u751f\u9577\u6700\u597d\uff0c\u4e26\u572836.5\u2103\u5b8c\u5168\u505c\u6b62\u3002 \u9019\u4e9b\u7d30\u80de\u8207\u6c38\u751f\u5316\u4eba\u985e\u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff08HMF3S\uff09\uff08abm\uff0cT0154\uff09\u4e0d\u540c\uff0c\u662f\u6eab\u5ea6\u654f\u611f\u7684\u3002 \u9019\u4e9b\u6709\u689d\u4ef6\u7684\u6c38\u751f\u7d30\u80de\u9069\u7528\u65bc\u7d30\u80de\u5de5\u7a0b\uff0c\u7406\u8ad6\u4e0a\u80fd\u5920\u7522\u751f\u4e0d\u673d\u7684\u4e8c\u500d\u9ad4\u4eba\u985e\u7d30\u80de\u682a\u3002\u66f4\u591a\u4e73\u817a\u7d30\u80de\u682a \u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff08HMF3A\uff09<\/u> | \u5c0f\u9f20\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\uff08iMMEC\uff09| \u4eba\u985e\u4e73\u817a\u5148\u9a45\u7d30\u80de–HTERT\uff08K5 + \/ K19 +\uff09<\/u><\/span><\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"immortalized<\/p>\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nMammary Tissue<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nSpindle<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nCombined transduction with retrovirus carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u672a\u547d\u540d-14\"<\/a><\/strong><\/span>\u00a0Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[3289],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13276"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13276"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13276\/revisions"}],"predecessor-version":[{"id":13281,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13276\/revisions\/13281"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13276"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13276"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13276"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}