{"id":13272,"date":"2017-08-27T18:42:30","date_gmt":"2017-08-27T10:42:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13272"},"modified":"2022-07-21T11:13:11","modified_gmt":"2022-07-21T03:13:11","slug":"%e4%ba%ba%e9%a1%9e%e4%b9%b3%e8%85%ba%e5%85%88%e9%a9%85%e7%b4%b0%e8%83%9e-htert%ef%bd%9cimmortalized-human-mammary-epithelial-progenitor-k5k19-cells-htert%e8%b2%a8%e8%99%9ft0452","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13272","title":{"rendered":"\u4eba\u985e\u4e73\u817a\u5148\u9a45\u7d30\u80de-HTERT\uff5cImmortalized Human Mammary Epithelial Progenitor (K5+\/K19+) Cells \u2013 hTERT\u8ca8\u865fT0452"},"content":{"rendered":"

HOT!!<\/b><\/span>\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

\u6c38\u751f\u5316\u7684\u4eba\u985e\u4e73\u817a\u5148\u9a45\u7d30\u80de–HTERT\uff08K5 + \/ K19 +\uff09\uff5c\u8ca8\u865fT0452<\/a><\/span><\/p>\n

Immortalized Human Mammary Epithelial Progenitor (K5+\/K19+) Cells \u2013 hTERT<\/strong><\/p>\n

\u6c38\u751f\u5316\u7684\u4eba\u985e\u4e73\u817a\u5148\u9a45\u7d30\u80de–HTERT\uff08K5 + \/ K19 +\uff09\u662f\u5171\u8868\u9054\u6b63\u5e38\u4e73\u817a\u548c\u5e79\u7d30\u80de\u6a19\u8a18\u7269\u7684\u5148\u9a45\u7d30\u80de\u7684\u514b\u9686\u7d30\u80de\u7fa4\u3002 \u5b83\u5177\u6709\u81ea\u6211\u66f4\u65b0\u548c\u5206\u5316\u6210\u7ba1\u8154\u548c\/\u6216\u808c\u4e0a\u76ae\u7d30\u80de\u8b5c\u7cfb\u7684\u80fd\u529b\u3002 \u901a\u904e\u5728\u4e0d\u540c\u512a\u5316\u57f9\u990a\u57fa\u4e2d\u7684\u7e41\u6b96\uff0c\u7d30\u80de\u80fd\u5920\u7522\u751f\u5177\u6709\u4e0d\u540c\u5f62\u614b\u548c\u7279\u5fb5\u7684\u7d30\u80de\uff0c\u5982mucin-1 positive cells, vimentin-negative cells, or expressing basal, luminal, \u548c\u5176\u4ed6\u5e79\u7d30\u80de\u6a19\u8a18\u7269\u3002 \u919b\u812b\u6c2b\u91761A3\u9176\u7684\u8868\u9054\u660e\u986f\u8f03\u9ad8\uff0c\u901a\u5e38\u7528\u65bc\u5206\u96e2\u6b63\u5e38\u548c\u816b\u7624\u4e73\u817a\u5e79\u7d30\u80de\u3002 K5 + \/ K19 +\u7d30\u80de\u5177\u6709Wnt\uff0cNotch\u548chedeghog\u57fa\u56e0\u8abf\u63a7\u9014\u5f91\u3002 \u5b83\u662f\u751f\u7269\u5b78\u7814\u7a76\u9818\u57df\u7684\u91cd\u8981\u5de5\u5177\uff0c\u4e3b\u8981\u4f86\u6e90\u65bc\u4e73\u817a\u764c\u548c\u5148\u9a45\u7d30\u80de\u7684\u7570\u8cea\u6027\u6a5f\u5236\u3002\u66f4\u591a\u4e73\u817a\u7d30\u80de\u682a \u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff08HMF3A\uff09<\/u> | \u5c0f\u9f20\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\uff08iMMEC\uff09| \u4eba\u985e\u4e73\u817a\u5148\u9a45\u7d30\u80de–HTERT\uff08K5 + \/ K19 +\uff09<\/u><\/span><\/p>\n

\"\"<\/a><\/p>\n

\"\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nMammary<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCobblestone or Spindle-shaped<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nK5, K14, vimentin, E-cadherin, p63, K8, K18, K19, CD29, CD49f a-SMA, CD10, Thy-1 when grown in DCFI-1<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nSerial passaging and transduction with recombinant lentiviruses carrying hTERT gene<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":13275,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[3290],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13272"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13272"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13272\/revisions"}],"predecessor-version":[{"id":13282,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13272\/revisions\/13282"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/13275"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13272"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}