{"id":13268,"date":"2017-08-27T17:39:50","date_gmt":"2017-08-27T09:39:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13268"},"modified":"2025-03-21T14:54:45","modified_gmt":"2025-03-21T06:54:45","slug":"%e6%b0%b8%e7%94%9f%e4%ba%ba%e9%a1%9e%e8%89%af%e6%80%a7%e5%89%8d%e5%88%97%e8%85%ba%e4%b8%8a%e7%9a%ae%e7%b4%b0%e8%83%9e-sv40%ef%bd%9cimmortalized-human-benign-prostate-epithelial-cell-sv40%e8%b2%a8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13268","title":{"rendered":"\u6c38\u751f\u4eba\u985e\u826f\u6027\u524d\u5217\u817a\u4e0a\u76ae\u7d30\u80de &#8211; SV40\uff5cImmortalized Human Benign Prostate Epithelial Cell &#8211; SV40\u8ca8\u865fT0002"},"content":{"rendered":"<p><span style=\"color: #ff0000;\"><b>HOT!!<\/b><\/span><a href=\"http:\/\/biopioneer.com.tw\/?p=5545\">\u00a0<span style=\"color: #0000ff;\">\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a><span style=\"color: #0000ff;\">\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #0000ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5582\">DAPI\u67d3\u6838<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #0000ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5564\">\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #0000ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5567\">\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #0000ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5575\">\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=13268\" target=\"_blank\" rel=\"noopener\">\u6c38\u751f\u4eba\u985e\u826f\u6027\u524d\u5217\u817a\u4e0a\u76ae\u7d30\u80de &#8211; SV40\uff5c\u8ca8\u865fT0002<\/a><\/span><\/p>\n<p><strong>Immortalized Human Benign Prostate Epithelial Cell &#8211; SV40<\/strong><\/p>\n<p>\u7d30\u80deAdherent \/ Polygonal | \u4f86\u6e90Prostate |\u3002\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (<a href=\"http:\/\/biopioneer.com.tw\/?p=7046\">\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (<a href=\"http:\/\/biopioneer.com.tw\/?p=7031\">\u8ca8\u865fG422<\/a>)\u3002\u7d30\u80de\u57f9\u990a\u57fa\u70baPrigrow II (<a href=\"https:\/\/www.abmgood.com\/Prigrow-II-Medium-TM002.html\">TM002<\/a>..) Atmosphere: air, 95%; Carbon dioxide (CO2), 5%, 10\uff05\u80ce\u725b\u8840\u6e05\uff08TM999\uff09\u3002 Atmosphere: air: 95%, CO\u2082: 5%; Temperature: 37.0\u00b0C.<br \/>\n<a href=\"https:\/\/www.abmgood.com\/admin\/IHCpics\/T0002cc.jpg\"><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/admin\/IHCpics\/T0002cc.jpg\" alt=\"\" width=\"100%\" \/><\/a><\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>BioSafety Level<\/strong><\/td>\n<td>II<\/td>\n<\/tr>\n<tr>\n<td><strong>Organism<\/strong><\/td>\n<td>Homo sapiens<\/td>\n<\/tr>\n<tr>\n<td><strong>Source Organ<\/strong><\/td>\n<td>Prostate<\/td>\n<\/tr>\n<tr>\n<td><strong>Donor Age<\/strong><\/td>\n<td>68<\/td>\n<\/tr>\n<tr>\n<td><strong>Donor Gender<\/strong><\/td>\n<td>Male<\/td>\n<\/tr>\n<tr>\n<td><strong>Growth Properties<\/strong><\/td>\n<td>Adherent<\/td>\n<\/tr>\n<tr>\n<td><strong>Morphology<\/strong><\/td>\n<td>Polygonal<\/td>\n<\/tr>\n<tr>\n<td><strong>Passage Number<\/strong><\/td>\n<td>10<\/td>\n<\/tr>\n<tr>\n<td><strong>Population Doubling<\/strong><\/td>\n<td>24-28 hours<\/td>\n<\/tr>\n<tr>\n<td><strong>Recommended Seeding Density<\/strong><\/td>\n<td>Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>For Research Use Only<\/td>\n<\/tr>\n<tr>\n<td><strong>Immortalization Method<\/strong><\/td>\n<td>Serial passaging and infection with ZipneoSV virus carrying SV40 T antigen gene<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><strong><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=12669\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/08\/%E6%9C%AA%E5%91%BD%E5%90%8D-14.jpg\" alt=\"\u672a\u547d\u540d-14\" \/><\/a><\/span>\u00a0\u00a0<\/strong><\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=6146\">Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=6152\">Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| <a href=\"http:\/\/biopioneer.com.tw\/?p=6159\">Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?p=6156\">Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":13269,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[3291],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13268"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13268"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13268\/revisions"}],"predecessor-version":[{"id":50840,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13268\/revisions\/50840"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/13269"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13268"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13268"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13268"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}