{"id":13262,"date":"2017-08-27T16:18:51","date_gmt":"2017-08-27T08:18:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13262"},"modified":"2025-03-21T14:55:25","modified_gmt":"2025-03-21T06:55:25","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e5%b0%8f%e9%bc%a0%e4%b9%b3%e8%85%ba%e4%b8%8a%e7%9a%ae%e7%b4%b0%e8%83%9e%ef%bc%88immec%ef%bc%89%ef%bd%9cimmortalized-mouse-mammary-epithelial-cells-immec%e8%b2%a8%e8%99%9f","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13262","title":{"rendered":"\u6c38\u751f\u5316\u5c0f\u9f20\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\uff08iMMEC\uff09\uff5cImmortalized Mouse Mammary Epithelial Cells (iMMEC)\u8ca8\u865fT0451"},"content":{"rendered":"

HOT!!<\/b><\/span>\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

\u6c38\u751f\u5316\u5c0f\u9f20\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\uff08iMMEC\uff09\uff5c\u8ca8\u865fT0451<\/a><\/span><\/p>\n

Immortalized Mouse Mammary Epithelial Cells (iMMEC)<\/span><\/b><\/p>\n

iMMEC<\/strong>\u7d30\u80de\u4ecb\u7d39\uff1a<\/strong>\u6c38\u751f\u5316\u5c0f\u9f20\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\uff08iMMEC\uff09\u662f\u901a\u904e\u817a\u75c5\u6bd2E1A\u548c\u986f\u6027\u9670\u6027p53\uff08p53DD\uff09\u7684\u5171\u540c\u8868\u9054\u800c\u884d\u751f\u7684\u7d30\u80de\u682a\u3002 \u9019\u7a2e\u7d30\u80de\u682a\u5177\u6709\u4ee5\u4e0b\u7368\u7279\u6027\uff1a\u5177\u8868\u9054\u8868\u9762\u6a19\u8a8c\u7269\uff0c\u5305\u62ec\u96cc\u6fc0\u7d20\u53d7\u9ad4\u03b1\uff08ER\u03b1\uff09\uff0c\u4e26\u4fdd\u7559\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\u7684\u529f\u80fd\u7279\u5fb5\u3002\u5728\u7522\u751f\u6ccc\u4e73\u523a\u6fc0\u6642\uff0c\u5f62\u6210\u6307\u793a\u4e73\u817a\u6ce1\u548c\u79d8\u5bc6\u7684\u5713\u9802\u72c0\u7d50\u69cb \u03b2-\u9023\u74b0\u86cb\u767d\u9032\u5165\u817a\u6ce1\u8154\u3002 \u8a72\u7d30\u80de\u9069\u7528\u65bc\u6fc0\u7d20\u8abf\u7bc0\u548c3D\u7dad\u5c0e\u7ba1\u5f62\u614b\u767c\u751f\u7814\u7a76\u3002\u66f4\u591a\u4e73\u817a\u7d30\u80de\u682a \u4e73\u817a\u6210\u7e96\u7dad\u7d30\u80de\uff08HMF3A\uff09<\/u> | \u5c0f\u9f20\u4e73\u817a\u4e0a\u76ae\u7d30\u80de\uff08iMMEC\uff09| \u4eba\u985e\u4e73\u817a\u5148\u9a45\u7d30\u80de–HTERT\uff08K5 + \/ K19 +\uff09<\/u><\/span><\/p>\n

\"\"<\/p>\n

\u7d30\u80deAdherent \/ Cuboidal | \u4f86\u6e90Mammary Epithelia | \u7d30\u80de\u6a19\u8a8c\u03b2-catenin, E-cadherin, Ep-CAM, CK8 and CK18\u3002\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002\u7d30\u80de\u57f9\u990a\u57fa\u70baPrigrow 4 ((TM004<\/a>.) Atmosphere: air, 95%; Carbon dioxide (CO2), 5%…\u88fd\u5099\u5b8c\u6210\u7684\u751f\u9577\u57f9\u990a\u57fa\uff1a10\uff05\u80ce\u725b\u8840\u6e05\uff08TM999\uff09\uff0c5\u03bcg\/ ml\u80f0\u5cf6\u7d20\uff0c1\u03bcg\/ ml hydrocortisone\u548c5ng \/ ml EGF\u3002 Atmosphere: air: 95%, CO\u2082: 5%; Temperature: 37.0\u00b0C.<\/p>\n

\"\"<\/p>\n

\u81f4\u764c\u523a\u6fc0\u53ef\u4ee5\u901a\u904e\u7528\u5206\u5316\u57f9\u990a\u57fa\u8a98\u5c0e\u7d30\u80de6\u5929\uff08\u6bcf2\u5929\u66f4\u63db\u4e00\u6b21\u57f9\u990a\u57fa\uff09\u4f86\u5b8c\u6210\u3002 \u88fd\u5099\u5b8c\u6574\u7684\u5206\u5316\u57f9\u990a\u57fa\uff0c\u5c07\u4ee5\u4e0b\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790ePrigorw IV\u57f9\u990a\u57fa\uff08TM004\uff09\u4e2d\uff1a5\u03bcg\/ ml\u80f0\u5cf6\u7d20\uff0c1\u03bcg\/ ml hydrocortisone\uff0c3\u03bcg\/ ml prolactin\u50ac\u4e73\u7d20\u548c2\uff05Matrigel\u3002\u4f7f\u7528Western blot\u6aa2\u6e2c\u6c38\u751f\u5316\u7d30\u80de\u7cfb\u4e2dE1A\u548cp53DD\u57fa\u56e0\u7684\u5b58\u5728\u3002 \u4ee5 Western blot\u548c\u514d\u75ab\u87a2\u5149\u6aa2\u6e2c\u03b2-\u9023\u74b0\u86cb\u767d\uff0cE-\u9223\u7c98\u86cb\u767d\uff0cEp-CAM\uff0cCK8\uff0cCK18\u548cER\u03b1\u7684\u57fa\u56e0\u8868\u9054\u3002<\/p>\n

\"immortalized<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\n6- to 8-week old C57B\/6 female mice<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nMammary Epithelia<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCuboidal<\/td>\n<\/tr>\n
Passage Number<\/strong><\/td>\n3<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\n\u03b2-catenin, E-cadherin, Ep-CAM, CK8 and CK18<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nSerial passaging and electroporation transfection with linearized DNA of E1A and p53DD<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/a>\"\u672a\u547d\u540d-14\"<\/a><\/strong><\/span>\u00a0Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[3292],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13262"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13262"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13262\/revisions"}],"predecessor-version":[{"id":50841,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13262\/revisions\/50841"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13262"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13262"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13262"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}