{"id":1320,"date":"2014-03-30T22:40:31","date_gmt":"2014-03-30T14:40:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=1320"},"modified":"2022-07-28T14:00:29","modified_gmt":"2022-07-28T06:00:29","slug":"abm-lentivirus-expression-system-%e6%85%a2%e7%97%85%e6%af%92%e8%a1%a8%e7%8f%be%e8%bc%89%e9%ab%94","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=1320","title":{"rendered":"\u3010\u75c5\u6bd2\u8f09\u9ad4\u3011Lentivirus Expression \u8868\u73fe\u8f09\u9ad4\u2014ABM good"},"content":{"rendered":"<p><span style=\"font-size: medium; color: #008000;\"><b>\u6162\u75c5\u6bd2\u7c21\u4ecb<\/b><\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u7684\u7d50\u69cb\u53ef\u5206\u6210\u5957\u819c\uff08envelop\uff09\u3001\u86cb\u767d\u8cea\u819c\uff08capsid\uff09\u3001\u4ee5\u53ca\u907a\u50b3\u7269\u8cea\u3002Lentivirus\u611f\u67d3\u5bbf\u4e3b\u5f8c\uff0c\u6703\u5c07\u5176\u57fa\u56e0\u5d4c\u5165\u5bbf\u4e3b\u7d30\u80de\u4f7f\u5176\u8868\u9054\u3002\u73fe\u70ba\u5be6\u9a57\u5ba4\u4e2d\u5e38\u898b\u7684\u57fa\u56e0\u91cd\u7d44\u5de5\u5177\u3002<\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u76ee\u524dlentivirus\u5df2\u53ef\u85c9\u7531\u3000<span style=\"color: #0000ff;\"><strong>1. \u5e36\u6709target gene\u7684lentivector<\/strong><\/span>\uff0c\u52a0\u4e0a\u3000<span style=\"color: #0000ff;\"><strong>2. packaging system<\/strong><\/span>\uff0c\u69cb\u6210\u7121\u81f4\u75c5\u6027\u7684psuedovirus\u3002<\/p>\n<p><span style=\"font-size: medium; color: #008000;\"><b>ABM Lentiviral Expression System \u6162\u75c5\u6bd2<\/b><b>\u8868\u73fe\u8f09\u9ad4<\/b><\/span><\/p>\n<ol>\n<li>\u4e0d\u50c5\u53ef\u505a\u70ba\u75c5\u6bd2\u8f09\u9ad4\u4f7f\u7528\uff0c\u4ea6\u53ef\u505a\u70ba\u4e00\u822c\u8f09\u9ad4\u9032\u884c\u8f49\u67d3\u3002<\/li>\n<li>\u5e36\u6709\u5169\u7a2e\u6297\u751f\u7d20\u7be9\u9078\u4f4d\uff0c\u4f9b\u539f\u6838\u6216\u771f\u6838\u751f\u7269\u4f7f\u7528\u3002<\/li>\n<li>\u591a\u6a23\u5316\u7684promoter\u9078\u64c7\uff08CMV\u3001UbC\u3001PGK\u3001EF1\u03b1\uff09\uff0c\u4ea6\u6709\u5c08\u4f9bpromoter\u7814\u7a76\u4e4bpromoterless\u8f09\u9ad4\u3002<\/li>\n<li>\u53ef\u4f9d\u4e0d\u540c\u5be6\u9a57\u9700\u6c42\u4f7f\u7528bicistronic\u3001tricistronic\uff0c\u6216\u662f\u5e36\u6709reporter gene \uff08GFP\u3001RFP\u3001Luciferase\uff09\u4e4b\u75c5\u6bd2\u8f09\u9ad4\u3002<\/li>\n<li>\u4f9d\u4e0d\u540c\u9700\u6c42\u751f\u7522\u5ba2\u88fd\u5316\u8f09\u9ad4\u3002<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u6162\u75c5\u6bd2\u7c21\u4ecb \u3000\u3000\u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u7684\u7d50\u69cb\u53ef\u5206\u6210\u5957\u819c\uff08envelop\uff09\u3001\u86cb\u767d\u8cea\u819c\uff08capsid\uff09\u3001\u4ee5 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":1338,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453,454,57,116,456,3],"tags":[4834],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/1320"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1320"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/1320\/revisions"}],"predecessor-version":[{"id":45957,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/1320\/revisions\/45957"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/1338"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1320"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1320"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1320"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}