{"id":13017,"date":"2017-08-21T16:30:44","date_gmt":"2017-08-21T08:30:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13017"},"modified":"2023-12-01T16:38:03","modified_gmt":"2023-12-01T08:38:03","slug":"gdnf%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-active-glial-cell-line-derived-neurotrophic-factor%e8%b2%a8%e8%99%9fapa043hu01-2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=13017","title":{"rendered":"GDNF\u6d3b\u6027\u91cd\u7d44\u86cb\u767d | Active Glial Cell Line Derived Neurotrophic Factor\u8ca8\u865fAPA043Hu01"},"content":{"rendered":"

\u81a0\u8cea\u7d30\u80de\u7cfb\u884d\u751f\u7684\u795e\u7d93\u71df\u990a\u56e0\u5b50 Active Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APA043Hu01<\/a><\/span><\/p>\n

\u81a0\u8cea\u7d30\u80de\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50 (Glial cell line-derived neurotrophic factor, GDNF) \u662f\u4e00\u7a2e\u795e\u7d93\u71df\u990a\u56e0\u5b50(neurotrophic factor)\uff0c\u5c6c\u65bc GDNF \u914d\u9ad4\u5bb6\u65cf (GFL)\uff0c\u88ab\u78ba\u5b9a\u70ba\u5e15\u91d1\u68ee\u6c0f\u75c7\u7684\u5019\u9078\u6cbb\u7642\u85e5\u7269\u3002 \u81a0\u8cea\u7d30\u80de\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50 (Glial cell line-derived neurotrophic factor, GDNF) \u662f\u4e00\u7a2e\u542b\u6709 211 \u500b\u6b98\u57fa\u7684 23.7 kDa \u86cb\u767d\u8cea\uff0c\u5728\u4fc3\u9032\u4e2d\u8166\u591a\u5df4\u80fa\u795e\u7d93\u5143\u7684\u5b58\u6d3b\u548c\u5206\u5316\u4e2d\u767c\u63ee\u95dc\u9375\u4f5c\u7528\u3002\u6b64\u5916\uff0c\u81a0\u8cea\u7d30\u80de\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50 (Glial cell line-derived neurotrophic factor, GDNF) \u4e5f\u88ab\u8b49\u660e\u53ef\u4ee5\u4fc3\u9032\u814e\u81df\u3001\u80f0\u81df\u548c\u80ba\u90e8\u7b49\u5468\u570d\u7d44\u7e54\u7684\u767c\u80b2\u3002\u6b64\u5916\uff0c\u81a0\u8cea\u7d30\u80de\u6e90\u6027\u795e\u7d93\u71df\u990a\u56e0\u5b50 (Glial cell line-derived neurotrophic factor, GDNF) \u4f5c\u70baGFL\u7684\u6210\u54e1\uff0c\u4e5f\u53c3\u8207\u816b\u7624\u7684\u9032\u5c55\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Glial Cell Line Derived Neurotrophic Factor (GDNF)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\u3002\u7d14\u5ea6> 90%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays.\u7b49\u4e0d\u540c\u7814\u7a76\u3002\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong>: \u86cb\u767d\u8cea\u6eb6\u65bc20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300 \u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg\/mL\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

\u6d3b\u6027\u6e2c\u8a66 (ACTIVITY TEST)\u00a0<\/span><\/h3>\n
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<\/p>\n

Figure 1. The binding activity of GDNF with GFRa2.<\/p>\n

\"APA043Hu01.png\"<\/p>\n

\"Active<\/p>\n<\/div>\n<\/div>\n

(GDNF)<\/strong>\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Homo sapiens (Human) Organism species<\/td>\nApplications<\/td>\n<\/tr>\n
APA043Hu01<\/td>\nActive Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
RPA043Hu01<\/td>\nRecombinant Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAA043Hu01<\/td>\nPolyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
LAA043Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
LAA043Hu81<\/td>\nFITC-Linked Polyclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nWB; IHC; ICC; IF.<\/td>\n<\/tr>\n
MAA043Hu22<\/td>\nMonoclonal Antibody to Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
SEA043Hu<\/td>\nELISA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
SCA043Hu<\/td>\nCLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)<\/strong><\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u81a0\u8cea\u7d30\u80de\u7cfb\u884d\u751f\u7684\u795e\u7d93\u71df\u990a\u56e0\u5b50 Active Glial Cell Line Derived Neurotrop […]<\/p>\n","protected":false},"author":3,"featured_media":6827,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5377,461],"tags":[3327],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13017"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13017"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13017\/revisions"}],"predecessor-version":[{"id":47569,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13017\/revisions\/47569"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6827"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13017"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13017"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13017"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}