{"id":12725,"date":"2017-08-11T22:38:03","date_gmt":"2017-08-11T14:38:03","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12725"},"modified":"2025-07-04T12:53:27","modified_gmt":"2025-07-04T04:53:27","slug":"12725","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=12725","title":{"rendered":"\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u5448\u8272\u6e2c\u5b9a | Beta-Lactamase Activity Colorimetric Assay Kit\u8ca8\u865fK803-100 (ABCAM ab197008)"},"content":{"rendered":"

\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biovision\u8ca8\u865fK803-100(ABCAM ab197008)<\/span><\/a><\/span><\/p>\n

Beta-Lactamase Activity Colorimetric Assay Kit<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39\uff1a\u03b2-\u5167\u91af\u80fa\u9176\uff08Beta-Lactamases, \u03b2Ls\uff09\u662f\u6c34\u89e3\u9176( hydrolases )\u5927\u5bb6\u65cf\uff0c\u5176\u5305\u542b\u8d85\u904e850\u500b\u9451\u5b9a\u7684\u6210\u54e1( identified members )\uff0c\u5176\u5728\u9769\u862d\u6c0f\u967d\u6027( Gram-positive )\u548c\u9769\u862d\u6c0f\u9670\u6027\u83cc(Gram-negative bacteria)\u4e2d\u8868\u9054\u3002\u03b2-\u5167\u91af\u80fa\u9176\uff08Beta-Lactamases, \u03b2Ls\uff09\u53ef\u4ee5\u6839\u64da\u5176\u5e95\u7269\u6216\u6291\u5236\u5291\u7279\u7570\u6027\u9032\u884c\u5206\u985e\u3002\u9019\u4e9b\u9176\u80fd\u5920\u6c34\u89e3\u7a31\u70ba\u03b2-\u5167\u91af\u80fa\u9176\uff08Beta-Lactamases, \u03b2Ls\uff09\u7684\u56db\u500b\u539f\u5b50\u74b0\u3002\u542b\u6709\u03b2-\u5167\u91af\u80fa\u9176\uff08Beta-Lactamases, \u03b2Ls\uff09\uff08\u5373\u9752\u9ef4\u7d20\uff0c\u982d\u5b62\u83cc\u7d20\uff0c\u55ae\u93c8\uff0c\u78b3\u9752\u9ef4\u70ef\uff09\u7684\u6297\u751f\u7d20\u9ad8\u5ea6\u6613\u65bc\u901a\u904e\u9176\u6d3b\u6027\u6c34\u89e3\uff0c\u5176\u4f7f\u5176\u6297\u751f\u7d20\u6548\u529b\u5931\u6d3b\u3002\u7531\u65bc\u51fa\u73fe\u03b2-\u5167\u91af\u80fa\u6297\u751f\u7d20\u6297\u6027\u7684\u7d30\u83cc\u83cc\u682a\u6578\u91cf\u9a5a\u4eba\uff0c\u03b2-\u5167\u91af\u80fa\u9176\uff08Beta-Lactamases, \u03b2Ls\uff09\u5df2\u7d93\u6210\u70ba\u91cd\u8981\u7684\u81e8\u5e8a\u5a01\u8105\u3002 BioVision\u7684\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u9748\u654f\u7684\u6e2c\u5b9a\u6cd5\uff0c\u53ef\u4ee5\u6aa2\u6e2c\u548c\u5b9a\u91cf\u9019\u4e9b\u6c34\u89e3\u9176\u7684\u9176\u6d3b\u6027\u3002\u8a72\u6e2c\u5b9a\u662f\u57fa\u65bc\u7522\u751f\u8207\u03b2L\u6d3b\u6027\u91cf\u6210\u6b63\u6bd4\u7684\u6709\u8272\u7522\u7269\uff08OD 490nm\uff09\u7684Nitrocefin\uff08\u4e00\u7a2e\u986f\u8272\u6027\u982d\u5b62\u83cc\u7d20\uff09\u7684\u6c34\u89e3\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u4ee5\u5728\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u4f4e\u81f30.06mU\u7684\u9176\u6d3b\u6027\u3002<\/p>\n

\u76f8\u95dc\u5546\u54c1<\/span> <\/strong>Nitrocefin<\/a>\u00a0| \u03b2-\u5167\u91af\u80fa\u9176\u9176\u5448\u8272\u5206\u6790<\/a>\u00a0| \u03b2-\u5167\u91af\u80fa\u9176\u5e95\u7269<\/a>\u00a0| \u03b2-\u5167\u91af\u80fa\u9176\u9176\u6291\u5236\u5291\u7be9\u9078<\/a>\u00a0| \u5feb\u901f\u03b2-\u5167\u91af\u80fa\u9176\u9176\u6d3b\u6027<\/u> | 384well \u03b2-\u5167\u91af\u80fa\u9176\u9176\u5448\u8272\u5206\u6790<\/a><\/span><\/span><\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/td>\nK803-100 (ABCAM ab197008)<\/td>\n<\/tr>\n
Size<\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/td>\nAbsorbance (490 nm)<\/td>\n<\/tr>\n
Applications<\/td>\n\u2022 Measurement of \u03b2-Lactamase activity in various biological samples \u2022 Analysis of \u03b2-Lactamase activity in pathological conditions<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Rapid, simple & sensitive \u2022 The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 \u03b2L Assay Buffer \u2022 Nitrocefin (in DMSO) \u2022 Positive Control (Lyophilized) \u2022 \u03b2L Hydrolysis Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Penwell et al., Molecular Mechanisms of Sulbactam Antibacterial Activity and Resistance Determinants in\u00a0Acinetobacter baumannii.\u00a0<\/em>Antimicrob.\u00a0Agents Chemother.,\u00a0Mar 2015; 59: 1680 – 1689.<\/a><\/p>\n

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\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u3002<\/strong><\/div>\n
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\u5927\u8178\u687f\u83cc\u57f9\u990a\u7269 (5 \u00b5l)\u3001\u53d7\u6c61\u67d3\u7684\u57f9\u990a\u57fa\uff08CM\uff1b30 \u00b5l\uff09\u548c\u967d\u6027\u5c0d\u7167\uff084 \u00b5l\uff09\u4e2d\u7684 \u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u3002<\/p>\n

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\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u3002<\/strong><\/div>\n
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\u5927\u8178\u687f\u83cc\u548c\u6c61\u67d3\u57f9\u990a\u57fa\u7684\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u4ee5\u6bcf\u6beb\u514b\u86cb\u767d\u8cea\u8868\u793a\u3002<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u03b2-\u5167\u91af\u80fa\u9176\u6d3b\u6027\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biovision\u8ca8\u865fK803-100(ABCAM ab197008) B […]<\/p>\n","protected":false},"author":3,"featured_media":6786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[486,5380],"tags":[3367],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12725"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12725"}],"version-history":[{"count":9,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12725\/revisions"}],"predecessor-version":[{"id":51983,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12725\/revisions\/51983"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6786"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12725"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12725"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12725"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}