{"id":12481,"date":"2017-08-04T13:32:05","date_gmt":"2017-08-04T05:32:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12481"},"modified":"2022-07-21T11:30:00","modified_gmt":"2022-07-21T03:30:00","slug":"rna%e8%9b%8b%e7%99%bd%e4%ba%a4%e8%81%af%e8%a9%a6%e5%8a%91-trauts-reagent%e5%a4%9a%e9%86%a3%e7%9a%84%e6%9c%89%e6%95%88%e7%a1%ab%e9%86%87%e5%8c%96%e8%a9%a6%e5%8a%91-%e8%b2%a8%e8%99%9f2330","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=12481","title":{"rendered":"RNA\u86cb\u767d\u4ea4\u806f\u8a66\u5291 |Traut’s Reagent\u591a\u91a3\u7684\u6709\u6548\u786b\u9187\u5316\u8a66\u5291 \u8ca8\u865f2330"},"content":{"rendered":"

Crosslinking<\/strong>\u8a66\u5291\u7cfb\u5217\u5546\u54c1<\/strong><\/p>\n

Traut’s Reagent <\/strong>| <\/strong>\u8ca8\u865f<\/strong>2330<\/a><\/span><\/p>\n

A useful reagent for converting primary amine functional groups into free sulfhydryl groups<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Traut’s Reagent\u70ba\u6c34\u6eb6\u6027\uff0c\u6dfb\u52a05-atom spacer arm (8.1 A\u030a)\u4ee5\u6700\u5c0f\u5316\u7a7a\u9593\u4f4d\u963b\u2022spacer\u9593\u9694\u7269\u5728\u4e8c\u786b\u9375\u7b49\u4e4b\u9593\u53ef\u88ab\u9084\u539f\u5291\u5207\u5272\uff0c\u5982DTT\uff0cTCEP\u3002\u901a\u5e38\u7528\u65bc\u5c07primary amines\u8f49\u5316\u70basulfhydryl groups\u5df0\u57fa\u3002 \u65bc\u7b2c\u4e00\u6b65\u904e\u7a0b\u4e2d\u7a31\u70ba\u786b\u9187\u5316\uff0c\u6c28\u57fa\u53cd\u61c9\u6027\u70bapH7-10\uff0c\u4e5f\u662f\u591a\u91a3\u7684\u6709\u6548\u786b\u9187\u5316\u8a66\u5291\u2022\u70baRNA\u86cb\u767d\u4ea4\u806f\u8a66\u5291\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\n2-Iminothiolane HCl; 2-IT; Dihydro-2-(3H)-thiophenimine hydrochloride; Dihydro-2-(3H)-thiophenimine HCl<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nWhite solid<\/td>\n<\/tr>\n
CAS #<\/strong><\/td>\n4781-83-3<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC\u2084H\u2087NS\u2022HCl<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n137.63<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n\u226590% by NMR<\/td>\n<\/tr>\n
Solubility<\/strong><\/td>\nH2O or aqueous buffers<\/td>\n<\/tr>\n
SMILES<\/strong><\/td>\nC1CC(=N)SC1.Cl<\/td>\n<\/tr>\n
InChi<\/strong><\/td>\nInChI=1S\/C4H7NS.ClH\/c5-4-2-1-3-6-4;\/h5H,1-3H2;1H<\/td>\n<\/tr>\n
InChi Key<\/strong><\/td>\nATGUDZODTABURZ-UHFFFAOYSA-N<\/td>\n<\/tr>\n
PubChem CID<\/strong><\/td>\n13166855<\/a><\/td>\n<\/tr>\n
MDL Number<\/strong><\/td>\nMFCD00039013<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nProtect from moisture<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/span> Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Crosslinking\u8a66\u5291\u7cfb\u5217\u5546\u54c1 Traut’s Reagent | \u8ca8\u865f2330 A use […]<\/p>\n","protected":false},"author":3,"featured_media":5468,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3401],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12481"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12481"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12481\/revisions"}],"predecessor-version":[{"id":14634,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12481\/revisions\/14634"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5468"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12481"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12481"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12481"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}