{"id":12366,"date":"2017-08-04T11:23:42","date_gmt":"2017-08-04T03:23:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12366"},"modified":"2023-02-09T12:24:51","modified_gmt":"2023-02-09T04:24:51","slug":"%e3%80%90abmgood%e4%b8%8d%e6%9c%bd%e5%8c%96%e7%b3%bb%e5%88%97%e5%95%86%e5%93%81%e3%80%91%e5%93%ba%e4%b9%b3%e5%8b%95%e7%89%a9%e7%b4%b0%e8%83%9e%e7%9a%84%e4%b8%8d%e6%9c%bd%e5%8c%96immortalized%e7%ad%96","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=12366","title":{"rendered":"\u3010Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1\u3011\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u4e0d\u673d\u5316Immortalized\u7b56\u7565-ABMGOOD"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/span><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong><\/span><\/p>\n

\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002 Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3406],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12366"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12366"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12366\/revisions"}],"predecessor-version":[{"id":38203,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12366\/revisions\/38203"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12366"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12366"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12366"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}