{"id":12316,"date":"2017-08-01T15:50:50","date_gmt":"2017-08-01T07:50:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12316"},"modified":"2022-07-21T13:46:43","modified_gmt":"2022-07-21T05:46:43","slug":"t7%e6%a0%b8%e9%85%b8%e5%85%a7%e5%88%87%e9%85%b6i-t7-endonuclease-i-%e8%b2%a8%e8%99%9f-g696","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=12316","title":{"rendered":"T7\u6838\u9178\u5167\u5207\u9176I | ABMGOOD T7 Endonuclease\u8ca8\u865f E028"},"content":{"rendered":"

T7 Endonuclease I<\/strong> | <\/strong>\u8ca8\u865f<\/strong>\u00a0E028\u00a0 1000 U (100 \u03bcl)<\/strong><\/a><\/span><\/p>\n

T7\u6838\u9178\u5167\u5207\u9176I (T7 Endonuclease I) \u8b58\u5225\u548c\u5207\u5272\u932f\u914d\u7684DNA\u3001\u7570\u6e90\u96d9\u93c8DNA\u3001cruciform DNA\u3001Holliday\u7d50\u69cb\u548c\/\u6216junctions\uff0c\u4ee5\u53canicked\u5207\u53e3\u96d9\u93c8DNA\u3002 \u8a72\u9176\u512a\u5148\u5207\u5272\u55ae\u93c8\u8d85\u96d9\u93c8DNA\uff0c\u5728\u7b2c\u4e00\uff0c\u7b2c\u4e8c\u6216\u7b2c\u4e09\u78f7\u9178\u4e8c\u916f\u9375\u8655\u767c\u751f\u5207\u5272\uff0c\u5176\u70ba5\u00b4 to the mismatch.<\/p>\n

\u9175\u7d20\u4e4b\u61c9\u7528<\/strong>: <\/strong>\u7a81\u8b8a\u6aa2\u6e2c\u6cd5\u3001\u6aa2\u6e2c\u548c\u5207\u5272\u7570\u6e90\u96d9\u93c8\u9ad4\u548c\/\u6216\u5207\u53e3DNA\u3001\u96a8\u6a5f\u5207\u5272DNA\u7528\u65bcshotgun cloning<\/p>\n\n\n\n\n\n\n\n
Form<\/strong><\/td>\nEnzyme supplied with 10X Reaction Buffer<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n10 U\/\u03bcl<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nStore all components at -20\u00b0C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton\u00ae X-100 and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n
Notes<\/strong><\/td>\nOne unit is defined as the amount of T7 Endonuclease I that linearizes over 90% of 1 \u00b5g of supercoiled cruciform DNA in a 50 \u00b5l volume in 1 hour at 37\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83\u5546\u54c1\u00a0<\/strong><\/span>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

T7 Endonuclease I | \u8ca8\u865f\u00a0E028\u00a0 1000 U (100 \u03bcl) T7\u6838\u9178\u5167\u5207\u9176I ( […]<\/p>\n","protected":false},"author":3,"featured_media":10635,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[3421],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12316"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12316"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12316\/revisions"}],"predecessor-version":[{"id":26311,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12316\/revisions\/26311"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10635"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12316"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12316"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}