{"id":12101,"date":"2017-07-20T15:08:18","date_gmt":"2017-07-20T07:08:18","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12101"},"modified":"2022-12-07T15:42:51","modified_gmt":"2022-12-07T07:42:51","slug":"12101","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=12101","title":{"rendered":"Mouse Anti-Human IgG1 Hinge-HRP\u6297\u9ad4 | Southernbiotech\u8ca8\u865f9052-05"},"content":{"rendered":"

Mouse Anti-Human IgG1 Hinge-HRP\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong><\/a>9052-05<\/a>\u00a0 \u00a01 ml<\/strong><\/span><\/p>\n

\"SB\"<\/a><\/p>\n

ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG1 Hinge-HRP (SB Cat. No. 9052-05).<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\n4E3<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-HRP<\/a><\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nHuman IgG1<\/sub>\u00a0Hinge<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nSupplied in a stock solution in 50% glycerol\/50% PBS, pH 7.4; No preservative added<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\nStore at 2-8\u00b0C
\nPlease refer to product specific\u00a0(M)SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

ELISA \u2013 Quality tested\u00a01-7,9<\/sup>
\nFLISA \u2013 Quality tested
\nFlow Cytometry \u2013 Reported in literature\u00a013-15<\/sup>
\nImmunohistochemistry-Paraffin Sections \u2013 Reported in literature\u00a08-10<\/sup>
\nImmunoblotting \u2013 Reported in literature\u00a011,12<\/sup>
\nMultiplex \u2013 Reported in literature\u00a016-19<\/sup>
\nSeparation \u2013 Reported in literature\u00a020<\/sup>
\nPurification \u2013 Reported in literature\u00a021<\/sup><\/p>\n

Note \u2013 For direct immunostaining of IgG1<\/sub>\u00a0positive cells in flow cytometry applications, clone HP6001 (SB Cat. No. 9054) is recommended<\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/p>\n

    \n
  1. Whary MT, Sundina N, Bravo LE, Correa P, Quinones F, Caro F, et al. Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev. 2005;14:1464-9. (ELISA)<\/li>\n
  2. Njoku DB, Mellerson JL, Talor MV, Kerr DR, Faraday NR, Outschoorn I, et al. Role of CYP2E1 immunoglobulin G4 subclass antibodies and complement in pathogenesis of idiosyncratic drug-induced hepatitis. Clin Vaccine Immunol. 2006;13:258-65. (ELISA)<\/li>\n
  3. Liu L, Chen M, Yu F, Zhao M, Wang H. IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener’s granulomatosis and microscopic polyangiitis. Nephrology. 2008;13:629-35. (ELISA)<\/li>\n
  4. Xu P, Cui Z, Chen M, Hellmark T, Zhao M. Comparison of characteristics of natural autoantibodies against myeloperoxidase and anti-myeloperoxidase autoantibodies from patients with microscopic polyangiitis. Rheumatology. 2011;50:1236-43. (ELISA)<\/li>\n
  5. Geisler WM, Morrison SG, Doemland ML, Iqbal SM, Su J, Mancevski A, et al. Immunoglobulin-specific responses to Chlamydia elementary bodies in individuals with and at risk for genital chlamydial infection. J Infect Dis. 2012;206:1836-43. (ELISA)<\/li>\n
  6. Shao C, Huo N, Zhao L, Gao Y, Fan X, Zheng Y, et al. The presence of thyroid peroxidase antibody of IgG2 subclass is a risk factor for thyroid dysfunction in chronic hepatitis C patients. Eur J Endocrinol. 2013;168:717-22. (ELISA)<\/li>\n
  7. Haag S, Schneider N, Mason DE, Tuncel J, Andersson IE, Peters EC, et al. Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. Arthritis Rheumatol. 2014;66:1440-9. (ELISA)<\/li>\n
  8. Qu Z, Liu G, Li J, Wu L, Tan Y, Zheng X, et al. Absence of glomerular IgG4 deposition in patients with membranous nephropathy may indicate malignancy. Nephrol Dial Transplant. 2012;27:1931-7. (IHC-PS)<\/li>\n
  9. Jia X, Hu S, Chen J, Qu Z, Liu G, Cui Z, et al. The clinical and immunological features of patients with combined anti-glomerular basement membrane disease and membranous nephropathy. Kidney Int. 2014;85:945-52. (ELISA, IHC-PS)<\/li>\n
  10. Qu Z, Cui Z, Liu G, Zhao M. The distribution of IgG subclass deposition on renal tissues from patients with anti-glomerular basement membrane disease. BMC Immunol. 2013;14:19. (IHC-PS)<\/li>\n
  11. B\u00f8e AS, Bredholt G, Knappskog PM, Hjelmervik TO, Mellgren G, Winqvist O, et al. Autoantibodies against 21-hydroxylase and side-chain cleavage enzyme in autoimmune Addison’s disease are mainly immunoglobulin G1. Eur J Endocrinol. 2004;150:49-56. (WB)<\/li>\n
  12. Skorstad G, Hestvik AL, Torjesen P, Alvik K, Vartdal F, Vandvik B, et al. GAD65 IgG autoantibodies in stiff person syndrome: clonality, avidity and persistence. Eur J Neurol. 2008;15:973-80. (WB)<\/li>\n
  13. Wang M, Qiu Y, Wang X, Zhao F, Jin M, Xu M, et al. Role of HLA-G and NCR in protection of umbilical cord blood haematopoietic stem cells from NK cell mediated cytotoxicity. J Cell Mol Med. 2011;15:2040-5. (FC)<\/li>\n
  14. Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014;14:1581-91. (FC)<\/li>\n
  15. Toyoda C, Suzuki Y, Tsuneyama H, Onodera T, Masuno A, Yabe R, et al. Production of human monoclonal anti-Jk3, recognising an epitope including the Jka\/Jkb polymorphic site of the Kidd glycoprotein. Transfus Med. 2014;24:286-91. (FC)<\/li>\n
  16. Keynan Y, Bodnarchuk T, Wayne S, Li Y, Fowke KR. Evaluation of influenza-specific humoral response by microbead array analysis. Can J Infect Dis Med Microbiol. 2011;22:25-9. (Multiplex)<\/li>\n
  17. Kaneku H, O’Leary JG, Taniguchi M, Susskind BM, Terasaki PI, Klintmalm GB. Donor-specific human leukocyte antigen antibodies of the immunoglobulin G3 subclass are associated with chronic rejection and graft loss after liver transplantation. Liver Transpl. 2012;18:984-92. (Multiplex)<\/li>\n
  18. Lowe D, Higgins R, Zehnder D, Briggs DC. Significant IgG subclass heterogeneity in HLA-specific antibodies: Implications for pathogenicity, prognosis, and the rejection response. Hum Immunol. 2013;74:666-72. (Multiplex)<\/li>\n
  19. Freitas MC, Rebellato LM, Ozawa M, Nguyen A, Sasaki N, Everly M, et al. The role of immunoglobulin-G subclasses and C1q in de novo HLA-DQ donor-specific antibody kidney transplantation outcomes. Transplantation. 2013;95:1113-9. (Multiplex)<\/li>\n
  20. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (Sep)<\/li>\n
  21. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (Purification)<\/li>\n<\/ol>\n

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     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse Anti-Human IgG1 Hinge-HRP\u6297\u9ad4 | \u8ca8\u865f9052-05\u00a0 \u00a01 ml EL […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[3435],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12101"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12101"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12101\/revisions"}],"predecessor-version":[{"id":36921,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12101\/revisions\/36921"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12101"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12101"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12101"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}