{"id":11859,"date":"2017-07-12T17:18:54","date_gmt":"2017-07-12T09:18:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11859"},"modified":"2022-07-21T13:58:01","modified_gmt":"2022-07-21T05:58:01","slug":"phospho-egfr-ser1070-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk4211-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11859","title":{"rendered":"Phospho-EGFR (Ser1070) ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fK4211-100"},"content":{"rendered":"<p><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=11859\">Phospho-EGFR (Ser1070) ELISA\u8a66\u5291\u76d2 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K4211-100<\/a><\/span><\/p>\n<p>An Sandwich ELISA kit for the quantitative measurement of phosoho-EGFR (Ser1070) in human serum, plasma, and other biological fluids<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/4\/k4246.jpg\" alt=\"\" \/><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>Phospho-EGFR\uff08Ser1070\uff09ELISA\uff08\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\uff09\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u975e\u5e38\u5feb\u901f\uff0c\u65b9\u4fbf\u548c\u9748\u654f\u7684\u6e2c\u5b9a\u8a66\u5291\u76d2\uff0c\u53ef\u4ee5\u76e3\u6e2c\u7d30\u80de\u88c2\u89e3\u7269\u4e2d\u91cd\u8981\u751f\u7269\u5b78\u9014\u5f91\u7684\u6d3b\u5316\u6216\u529f\u80fd\u3002\u53ef\u78ba\u5b9a\u5be6\u9a57\u6a21\u578b\u7cfb\u7d71\u4e2d\u7684\u78f7\u9178\u5316EGFR\u86cb\u767d\uff0c\u9a57\u8b49\u7d30\u80de\u88c2\u89e3\u7269\u662f\u5426\u6d3b\u5316\u3002\u53ef\u540c\u6642\u6e2c\u91cf\u8a31\u591a\u4e0d\u540c\u7684\u7d30\u80de\u88c2\u89e3\u7269\uff0c\u800c\u4e0d\u7528\u82b1\u8cbb\u592a\u591a\u6642\u9593\u548c\u7cbe\u529b\u9032\u884cWestern Blot\u5206\u6790\u3002\u8a72\u4e09\u660e\u6cbbELISA\u8a66\u5291\u76d2\u662f\u7528\u65bc\u6e2c\u91cf\u4eba\u985e\u78f7\u9178\u5316EGFR\uff08Ser1070\uff09\u7684\u9ad4\u5916\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\u6cd5\u3002\u6297EGFR\u6297\u9ad4\u5df2\u7d93\u88ab\u5857\u8986\u523096\u5b54\u677f\u4e0a\u3002\u5c07\u6a23\u54c1\u79fb\u5165\u5b54\u4e2d\uff0c\u4e26\u5c07\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u78f7\u9178\u5316\u548c\u672a\u78f7\u9178\u5316\u7684EGFR\u901a\u904e\u56fa\u5b9a\u7684\u6297\u9ad4\u7d50\u5408\u5230\u5b54\u3002\u6d17\u6ecc\u5b54\uff0c\u4f7f\u7528\u6297\u78f7\u9178-EGFR\uff08Ser1070\uff09\u6297\u9ad4\u6aa2\u6e2c\u78f7\u9178\u5316EGFR\u3002\u6d17\u53bb\u672a\u7d50\u5408\u7684\u6297\u9ad4\u5f8c\uff0c\u5c07HRP\u7db4\u5408\u7684\u6297\u5154IgG\uff08\u4e8c\u6297\uff09\u79fb\u6db2\u81f3\u5b54\u4e2d\u3002\u518d\u6b21\u6d17\u6ecc\u5b54\uff0c\u5411\u5b54\u4e2d\u52a0\u5165TMB\u5e95\u7269\u6eb6\u6db2\uff0c\u4e26\u8207\u6240\u7d50\u5408\u7684EGFR\u7684\u91cf\u6210\u6bd4\u4f8b\u5730\u986f\u8272\u3002 Stop Solution\u5c07\u984f\u8272\u5f9e\u85cd\u8272\u8b8a\u70ba\u9ec3\u8272\uff0c\u4e26\u4e14\u5728450nm\u8655\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002<\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K4246-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Absorbance (450 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Human<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Quantitative measurement of phosoho-EGFR (Ser1070) in human cell lysates<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>Easy, convenient and time-saving method to measure the level of phosoho-EGFR (Ser1070) in human cell lysates<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 EGFR Microplate (Item A) coated with monoclonal anti-EGFR<br \/>\n\u2022 Wash Buffer Concentrate (20X) (Item B)<br \/>\n\u2022 Assay Diluent (5X) (Item E2)<br \/>\n\u2022 Anti-phospho-EGFR (Ser1070) (Item C)<br \/>\n\u2022 HRP-conjugated Anti-rabbit IgG (1000X) (Item D-1)<br \/>\n\u2022 TMB One-Step Substrate Reagent (Item H)<br \/>\n\u2022 Stop Solution (Item I)<br \/>\n\u2022 Cell Lysate Buffer (2X) (Item J)<br \/>\n\u2022 Positive Control A431S001-1 (Item K)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Phospho-EGFR (Ser1070) ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fK4211-100 An Sandwi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":10325,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[462],"tags":[3467],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11859"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11859"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11859\/revisions"}],"predecessor-version":[{"id":11862,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11859\/revisions\/11862"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10325"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11859"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11859"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11859"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}