{"id":11806,"date":"2017-07-11T15:58:55","date_gmt":"2017-07-11T07:58:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11806"},"modified":"2022-07-21T14:00:11","modified_gmt":"2022-07-21T06:00:11","slug":"%e7%b5%84%e8%9b%8b%e7%99%bd%e4%b9%99%e9%85%b0%e8%bd%89%e7%a7%bb%e9%85%b6%ef%bc%88hats%ef%bc%89%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90-hat-activity-colorimetric-assay-kit-%e8%b2%a8%e8%99%9fk332","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11806","title":{"rendered":"\u7d44\u86cb\u767d\u4e59\u91af\u8f49\u79fb\u9176\uff08HATs\uff09\u6d3b\u6027\u5206\u6790 | HAT Activity Colorimetric Assay Kit \u8ca8\u865fK332"},"content":{"rendered":"

HAT Activity Colorimetric Assay Kit | <\/strong>\u8ca8\u865f<\/strong>K332<\/a><\/span><\/p>\n

Non-radioactive assay for end point & kinetic studies<\/em><\/strong><\/p>\n

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\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u7d44\u86cb\u767d\u4e59\u91af\u8f49\u79fb\u9176\uff08Histone acetyltransferases, HATs\uff09\u5df2\u7d93\u88ab\u727d\u9023\u5728\u5404\u7a2e\u7d30\u80de\u529f\u80fd\u5982\u00a0gene transcription\u57fa\u56e0\u8f49\u9304\uff0cdifferentiation\u5206\u5316\u548cproliferation\u589e\u6b96\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\u3002 BioVision\u7684HAT\u6d3b\u6027\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u65b9\u4fbf\uff0c\u975e\u653e\u5c04\u6027\u7684\u7cfb\u7d71\uff0c\u53ef\u5feb\u901f\u9748\u654f\u5730\u6aa2\u6e2c\u54fa\u4e73\u52d5\u7269\u6a23\u54c1\u4e2d\u7684HAT\u6d3b\u6027\u3002 \u8a72\u8a66\u5291\u76d2\u5229\u7528active Nuclear Extract\u00a0\u6d3b\u6027\u6838\u63d0\u53d6\u7269\uff08NE\uff09\u4f5c\u70ba\u00a0positive control\u967d\u6027\u5c0d\u7167\uff0cacetyl-CoA\u4e59\u8f14\u9176A\u4f5c\u70ba\u8f14\u56e0\u5b50\u3002 \u901a\u904e\u6d3b\u6027HAT\u7684\u80bd\u5e95\u7269\u7684\u4e59\u9170\u5316\u91cb\u653e\u6e38\u96e2\u5f62\u5f0f\u7684CoA\uff0c\u5176\u7136\u5f8c\u7528\u4f5c\u7522\u751fNADH\u7684\u5fc5\u9700\u8f14\u9176\u3002 \u8207\u53ef\u6eb6\u6027tetrazolium dye\u56db\u5511\u9393\u67d3\u6599\u53cd\u61c9\u6642\uff0c\u53ef\u4ee5\u901a\u904e\u5206\u5149\u5149\u5ea6\u6cd5\u5bb9\u6613\u5730\u6aa2\u6e2cNADH\u3002 \u6aa2\u6e2c\u53ef\u4ee5\u662f\u9023\u7e8c\u6027\uff0c\u9069\u7528\u65bc\u52d5\u529b\u5b78\u7814\u7a76\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK332-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (440 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetects HAT activity by colorimetric method in mammalian samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 The detection can be continuous and suitable for kinetic studies<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 2X HAT Assay Buffer
\n\u2022 HAT Substrate I
\n\u2022 HAT Substrate II
\n\u2022 NADH Generating Enzyme
\n\u2022 HAT Reconstitution Buffer
\n\u2022 Nuclear Extract 4 mg\/ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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\u5f15\u7528\u6587\u737b<\/strong><\/div>\n
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Tzeng, Thing-Fong et al. (2016) Antioxidant-Rich Extract from Plantaginis Semen Ameliorates Diabetic Retinal Injury in a Streptozotocin-Induced Diabetic Rat Model,\u00a0Nutrients. 2016 Sep 18;8(9).<\/a><\/div>\n<\/div>\n
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Yang et al., PARP-1 Mediates LPS-Induced HMGB1 Release by Macrophages through Regulation of HMGB1 Acetylation.J. Immunol.,\u00a0Dec 2014; 193: 6114 – 6123.<\/a><\/div>\n<\/div>\n
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Khalyfa et al., Sleep Fragmentation During Late Gestation Induces Metabolic Perturbations and Epigenetic Changes in Adiponectin Gene Expression in Male Adult Offspring Mice.\u00a0Diabetes,\u00a0Oct 2014; 63: 3230 – 3241.<\/a><\/div>\n<\/div>\n
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Wang et al., Endothelial Cell Heparanase Taken Up by Cardiomyocytes Regulates Lipoprotein Lipase Transfer to the Coronary Lumen After Diabetes.\u00a0Diabetes,\u00a0Aug 2014; 63: 2643 – 2655.<\/a><\/div>\n<\/div>\n
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Vecellio et al., The Histone Acetylase Activator Pentadecylidenemalonate 1b Rescues Proliferation and Differentiation in the Human Cardiac Mesenchymal Cells of Type 2 Diabetic Patients.\u00a0Diabetes, Jun 2014; 63: 2132 \u2013 2147.<\/a><\/div>\n<\/div>\n

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HAT Activity Colorimetric Assay Kit | \u8ca8\u865fK332 Non-radioa […]<\/p>\n","protected":false},"author":3,"featured_media":10325,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[3476],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11806"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11806"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11806\/revisions"}],"predecessor-version":[{"id":18637,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11806\/revisions\/18637"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10325"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11806"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11806"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11806"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}