{"id":11780,"date":"2017-07-11T12:16:45","date_gmt":"2017-07-11T04:16:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11780"},"modified":"2022-07-21T14:00:45","modified_gmt":"2022-07-21T06:00:45","slug":"cell-surface-protein-isolation-kit-%e7%b4%b0%e8%83%9e%e8%a1%a8%e9%9d%a2%e8%9b%8b%e7%99%bd%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk295","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11780","title":{"rendered":"\u7d30\u80de\u8868\u9762\u86cb\u767d\u5206\u96e2\u8a66\u5291\u76d2 | Cell Surface Protein Isolation Kit \u8ca8\u865fK295-10"},"content":{"rendered":"

Cell Surface Protein Isolation Kit \u7d30\u80de\u8868\u9762\u86cb\u767d\u5206\u96e2\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K295<\/a><\/span><\/p>\n

Simple and efficient method for isolation of cell surface proteins<\/em><\/strong><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u7d30\u80de\u8868\u9762\u86cb\u767d(Cell surface proteins)\u5728\u4fe1\u865f\u8f49\u5c0e(\u00a0signal transduction)\uff0c\u7d30\u80de\u7c98\u9644(cell adhesion)\uff0c\u8f49\u904b(transport )\u4e2d\u8d77\u4e3b\u8981\u4f5c\u7528\uff0c\u4e26\u4f5c\u70ba\u8a3a\u65b7(diagnostic)\u548c\u85e5\u7406\u5b78(pharmacological targets)\u9776\u9ede\u3002 BioVision\u7684\u7d30\u80de\u8868\u9762\u86cb\u767d\u5206\u96e2\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u6709\u6548\u7684\u7d30\u80de\u8868\u9762\u86cb\u767d\u5206\u96e2\u65b9\u6cd5\u3002 \u5728\u8a72\u65b9\u6cd5\u4e2d\uff0c\u9996\u5148\u7528Sulfo-NHS-SS\u751f\u7269\u7d20\uff08\u00a0Sulfo-NHS-SS-Biotin), \u80fa\u53cd\u61c9\u6027 (thiol-cleavable)\uff0c\u786b\u9187\u53ef\u5207\u5272\u7684\u751f\u7269\u7d20\u5316\u8a66\u5291(thiol-cleavable\uff09\u6a19\u8a18\u7d30\u80de\u3002 \u96a8\u5f8c\u7d30\u80de\u88c2\u89e3\uff0c\u4f7f\u7528\u93c8\u9ef4\u6297\u751f\u7269\u7d20\u86cb\u767d\u73e0(Streptavidin beads)\u5206\u96e2\u6a19\u8a18\u7684\u7d30\u80de\u8868\u9762\u86cb\u767d\u8cea\u3002 \u7136\u5f8c\u901a\u904e\u8207DTT\u6eb6\u6db2\u5b75\u80b2\u5f9e\u73e0\u7c92\u91cb\u653e\u7d50\u5408\u7684\u86cb\u767d\u8cea\u3002 \u751f\u7269\u7d20\u5316\u8a66\u5291\u662f\u5177\u6709\u5ef6\u4f38\u9593\u9694\u81c2\u7684\u7d30\u80de\u819c\u4e0d\u53ef\u6ef2\u900f\u7684\uff0c\u4ee5\u6e1b\u5c11\u8207\u93c8\u9ef4\u89aa\u548c\u7d20\u7d50\u5408\u76f8\u95dc\u7684\u7a7a\u9593\u969c\u7919\u3002 \u9019\u7a2e\u65b9\u4fbf\u7684\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u6240\u6709\u5fc5\u9700\u7684\u7d44\u5206\uff0c\u7528\u65bc\u7d30\u80de\u8868\u9762\u86cb\u767d\u7684\u6700\u4f73\u6a19\u8a18\u548c\u5206\u96e2\u3002<\/p>\n

\"\"<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK295-10<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n10 reactions<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nWestern Blotting<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIsolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple and efficient method for the isolation of cell surface proteins
\n\u2022 Includes all required components for optimal labeling and isolation of the cell surface proteins<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Quenching Solution
\n\u2022 Lysis Buffer
\n\u2022 Wash Buffer
\n\u2022 Streptavidin Beads
\n\u2022 PBS Tablet
\n\u2022 TBS Tablet
\n\u2022 Sulfo-NHS-SS-Biotin
\n\u2022 DTT<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u5be6\u9a57\u8a2d\u50994\"<\/a><\/p>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Cell Surface Protein Isolation Kit \u7d30\u80de\u8868\u9762\u86cb\u767d\u5206\u96e2\u8a66\u5291\u76d2 | \u8ca8\u865fK295 […]<\/p>\n","protected":false},"author":3,"featured_media":10325,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3480],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11780"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11780"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11780\/revisions"}],"predecessor-version":[{"id":29614,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11780\/revisions\/29614"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10325"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11780"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11780"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11780"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}