{"id":11759,"date":"2017-07-10T16:54:04","date_gmt":"2017-07-10T08:54:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11759"},"modified":"2025-07-01T13:57:31","modified_gmt":"2025-07-01T05:57:31","slug":"peg%e7%97%85%e6%af%92%e5%8a%9b%e5%83%b9%e6%bf%83%e7%b8%ae%e5%a5%97%e7%b5%84-peg-virus-precipitation-kit-%e8%b2%a8%e8%99%9fk904-200","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11759","title":{"rendered":"PEG (\u805a\u4e59\u4e8c\u9187) \u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u5957\u7d44 |PEG Virus Precipitation Kit \u8ca8\u865fK904-200\/ab102538"},"content":{"rendered":"<p><span style=\"color: #ff6600; font-size: 12pt;\"><a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=11759\"><span style=\"font-size: 12pt;\"><strong><span style=\"color: #ff6600;\">PEG\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u5957\u7d44 | \u8ca8\u865fK904-200<\/span><\/strong><\/span><\/a><strong>\u00a0(5\u500d200\u500b\u53cd\u61c9)\u00a0\/ab102538<\/strong><\/span><\/p>\n<p><strong><em>PEG Virus Precipitation Kit<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>\u75c5\u6bd2\u901a\u5e38\u4ee5\u4f4e\u529b\u50f9\u7522\u751f\u3002Biovision\u63d0\u4f9b\u4f4e\u901f\u7c21\u55ae\u4e4b\u65b9\u6cd5\uff0c\u6fc3\u7e2e\u75c5\u6bd2\u529b\u50f9\u548c\u53bb\u9664\u96dc\u8cea\uff0c\u3002PEG\u75c5\u6bd2\u6c89\u6fb1\u8a66\u5291\u76d2\u7c21\u55ae\uff0c\u65b9\u4fbf\u548c\u7701\u6642\uff0c\u53ef\u4ee5\u5c07\u75c5\u6bd2\u6fc3\u7e2e\u800c\u7121\u9700\u8d85\u96e2\u5fc3\u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u5c0f\u5be6\u9a57\u5ba4\u6a23\u54c1\u6216\u5927\u898f\u6a21\u75c5\u6bd2\u88fd\u5099\uff0c\u7522\u91cf\u9ad8\uff0c\u75c5\u6bd2\u6ef4\u5ea6\u9ad8\u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u5c07\u7d30\u80de\u57f9\u990a\u57fa\u6216\u74b0\u5883\u6a23\u54c1\u4e2d\u7684\u9006\u8f49\u9304\u75c5\u6bd2(retroviruses)\uff0c\u687f\u72c0\u75c5\u6bd2(baculoviruses)\uff0c\u6162\u75c5\u6bd2(baculoviruses)\u548c\u566c\u83cc\u9ad4(lentiviruses)\u7b49\u9032\u884c\u6fc3\u7e2e\u3002 \u75c5\u6bd2\u53ef\u4ee5\u96c6\u4e2d\u5728100\u500d\u4ee5\u4e0a\u3002 \u6839\u64da\u75c5\u6bd2\u985e\u578b\u548c\u4f86\u6e90\uff0c\u63d0\u4f9b\u512a\u5316\u7684\u75c5\u6bd2\u518d\u61f8\u6d6e\u6db2\uff0c\u4f7f\u75c5\u6bd2\u6062\u5fa9\u6700\u5927\u5316\u905440-100\uff05\u3002\u6574\u500b\u904e\u7a0b\u4f7f\u7528\u7121\u6bd2\u8a66\u5291\uff0c\u6fc3\u7e2e\u75c5\u6bd2\u53ef\u7528\u65bc\u611f\u67d3\uff0c\u75c5\u6bd2DNA\u6216RNA\u7d14\u5316\u7b49\u61c9\u7528\u3002Virus was usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. BioVision\u2019s PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Virus can be concentrated over 100 folds. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/02\/PEG-%E8%81%9A%E4%B9%99%E4%BA%8C%E9%86%87-%E7%97%85%E6%AF%92%E5%8A%9B%E5%83%B9%E6%BF%83%E7%B8%AE%E5%A5%97%E7%B5%84PEG-Virus-Precipitation-Kit-%E8%B2%A8%E8%99%9FK904-200-ab102538.jpg\" \/><\/p>\n<div class=\"legend__title\"><span style=\"font-size: 12pt; color: #800000;\"><strong>\u529f\u80fd\u7814\u7a76 &#8211; PEG \u75c5\u6bd2\u6c89\u6fb1\u8a66\u5291\u76d2 (ab102538)<\/strong><\/span><\/div>\n<div class=\"legend__description\">\n<p>\u687f\u72c0\u75c5\u6bd2\u7684\u6fc3\u5ea6\uff1a\u4f9d\u8a66\u5291\u76d2\u65b9\u6848\u6c89\u6fb1\u8868\u9054\u4eba\u985e\u7c92\u9176B\u7684\u4f4e\u6ef4\u5ea6\u687f\u72c0\u75c5\u6bd2\uff0810ml\uff09\uff0c\u5c07\u6c89\u6fb1\u7269\u61f8\u6d6e\u57281ml\u75c5\u6bd2\u91cd\u61f8\u6db2\u4e2d\u3002\u96a8\u5f8c\u4f7f\u7528\u4f4e\u6ef4\u5ea6\u548c\u6c88\u6fb1\u7684\u687f\u72c0\u75c5\u6bd2\u611f\u67d3SF9\u6606\u87f2\u7d30\u80de\u3002\u4f7f\u7528<a href=\"https:\/\/www.abcam.com\/ab157403.html\">ab157403<\/a>\u00a0&#8211; \u7c92\u9176 B \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09\u76e3\u6e2c\u7531\u4f4e\u6ef4\u5ea6\u548c\u6c88\u6fb1\u7684\u687f\u72c0\u75c5\u6bd2\u611f\u67d3\u6606\u87f2\u7d30\u80de\u5206\u6ccc\u5230\u57f9\u990a\u57fa\u4e2d\u7684\u91cd\u7d44\u7c92\u9176 B \u7684\u6d3b\u6027\u3002<\/p>\n<\/div>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/05\/0-PEG\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u5957\u7d44\u8ca8\u865fK904-200-5\u500d200\u500b\u53cd\u61c9-ab102538.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>PEG<\/strong><strong>\u75c5\u6bd2\u6fc3\u7e2e\u5957\u7d44<\/strong><\/span><\/p>\n<p>PEG Virus Precipitation Kit PEG\u75c5\u6bd2\u6fc3\u7e2e\u7522\u54c1\uff0c\u6709\u6548\u63d0\u9ad8\u75c5\u6bd2\u529b\u50f9\u3002\u53ef\u61c9\u7528\u65bcLentivirus \u75c5\u6bd2\u6fc3\u7e2e\uff0c\u767b\u9769\u71b1\u75c5\u6bd2 (40~50nm)\u3001\u6d41\u611f\u75c5\u6bd2(~100nm)\u3001\u8178\u75c5\u6bd2(20~30nm)\u4e4b\u75c5\u6bd2\u9846\u7c92\u4e4b\u6fc3\u7e2e\u3002\u61c9\u7528\u65bc\u7d30\u80de\u57f9\u990a\u4e4b\u75c5\u6bd2\u6db2\u6fc3\u7e2e\u3001\u74b0\u5883sample\u75c5\u6bd2\u7684\u6fc3\u7e2e\u7b49\u61c9\u7528\u3002<\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>PEG<\/strong><strong>\u75c5\u6bd2\u6fc3\u7e2e\u7522\u54c1\u7279\u9ede:<\/strong><\/span><\/p>\n<ul>\n<li>\u4e0d\u9808\u8d85\u9ad8\u901f\u96e2\u5fc3<\/li>\n<li>\u5b89\u5168\u7121\u6bd2\u6027\uff0c\u6fc3\u7e2e\u5b8c\u4e4b\u75c5\u6bd2\u53ef\u76f4\u63a5\u611f\u67d3\u7d30\u80de\uff0c\u53ca\u9032\u884c\u4e0b\u6e38\u5206\u6790<\/li>\n<li>\u53ef\u5c07\u75c5\u6bd2\u6db2\u6fc3\u7e2e10 \u81f3100 \u500d\uff0c\u56de\u6536\u738740~100%<\/li>\n<li>\u9069\u7528\u65bc Lentivirus\u3001Phages\u3001\u767b\u9769\u71b1\u75c5\u6bd2\u3001\u6d41\u611f\u75c5\u6bd2\u3001\u8178\u75c5\u6bd2\u4e4b\u75c5\u6bd2\u9846\u7c92\u4e4b\u7b49\u75c5\u6bd2\u6fc3\u7e2e\u3002<\/li>\n<li>\u5305\u88dd\u70ba5 \u500d\u7121\u83cc\u6fc3\u7e2e\u6db2\uff0c\u6574\u7d44\u53ef\u7d14\u5316500ml\/2000ml\u75c5\u6bd2\u6db2\u00a0 (50\u6216200reaction, k904-50\/K904-200)<\/li>\n<\/ul>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 A quick, easy and inexpensive method is desired to concentrate virus and remove impurities<br \/>\n\u2022 Easy, convenient and time-saving method to concentrate virus without ultra-centrifugation<br \/>\n\u2022 Virus can be concentrated over 100 folds<br \/>\n\u2022 The whole process uses non-toxic reagents<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 PEG Solution (5X)<br \/>\n\u2022 Virus Re-suspension Solution (1X)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><span style=\"color: #800000;\"><span style=\"font-size: 12pt;\">\u5f15\u7528\u6587\u737b<\/span><\/span><\/strong><\/p>\n<p>Chanyang Kim, et al., (2018) IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K\/PDK-1\/Akt pathway,\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5843822\/?tool=pmcentrez&amp;report=abstract\">Endocrine Connections, May 2018, 29459421<\/a><\/p>\n<p>Andrews et al., High Preexisting Serological Antibody Levels Correlate with Diversification of the Influenza Vaccine Response.\u00a0<a href=\"http:\/\/jvi.asm.org\/content\/89\/6\/3308.abstract?maxtoshow=&amp;HITS=10&amp;hits=10&amp;RESULTFORMAT=&amp;andorexacttitle=and&amp;andorexacttitleabs=and&amp;fulltext=biovision&amp;andorexactfulltext=and&amp;searchid=1&amp;FIRSTINDEX=10&amp;fdate=3\/1\/2015&amp;tdate=3\/31\/2015&amp;resourcetype=HWCIT\">J. Virol.,\u00a0Mar 2015; 89: 3308 &#8211; 3317.<\/a><\/p>\n<p>Lee et al., First Report of\u00a0<em>Oryctes rhinoceros nudivirus<\/em>\u00a0(Coleoptera: Scarabaeidae) Causing Severe Disease in\u00a0<em>Allomyrina dichotoma<\/em>\u00a0in Korea.\u00a0<a href=\"http:\/\/jinsectscience.oxfordjournals.org\/content\/15\/1\/26.abstract?maxtoshow=&amp;HITS=10&amp;hits=10&amp;RESULTFORMAT=&amp;andorexacttitle=and&amp;andorexacttitleabs=and&amp;fulltext=biovision&amp;andorexactfulltext=and&amp;searchid=1&amp;FIRSTINDEX=0&amp;fdate=3\/1\/2015&amp;tdate=3\/31\/2015&amp;resourcetype=HWCIT\">J Insect Sci,\u00a0Mar 2015; 15: 26.<\/a><\/p>\n<p>Warnes et al., Inactivation of Murine Norovirus on a Range of Copper Alloy Surfaces Is Accompanied by Loss of Capsid Integrity.\u00a0<a href=\"http:\/\/aem.asm.org\/content\/81\/3\/1085.abstract?maxtoshow=&amp;HITS=10&amp;hits=10&amp;RESULTFORMAT=&amp;andorexacttitle=and&amp;andorexacttitleabs=and&amp;fulltext=biovision&amp;andorexactfulltext=and&amp;searchid=1&amp;FIRSTINDEX=10&amp;fdate=2\/1\/2015&amp;tdate=2\/28\/2015&amp;resourcetype=HWCIT\">Appl. Envir. Microbiol.,\u00a0Feb 2015; 81: 1085 &#8211; 1091.<\/a><\/p>\n<p>Busskamp et al., Rapid neurogenesis through transcriptional activation in human stem cells.\u00a0<a href=\"http:\/\/msb.embopress.org\/content\/10\/11\/760.abstract?maxtoshow=&amp;HITS=10&amp;hits=10&amp;RESULTFORMAT=&amp;andorexacttitle=and&amp;andorexacttitleabs=and&amp;fulltext=biovision&amp;andorexactfulltext=and&amp;searchid=1&amp;FIRSTINDEX=0&amp;fdate=11\/1\/2014&amp;tdate=11\/30\/2014&amp;resourcetype=HWCIT\">Mol Syst Biol,\u00a0Nov 2014; 10: 760.<\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/11\/%E6%8A%80%E8%A1%93%E6%94%AF%E6%8F%B4%E7%AF%87-300x105.jpg\" alt=\"\u6280\u8853\u652f\u63f4\u7bc7\" width=\"158\" height=\"54\" \/><\/p>\n<p>a) \u5c0d\u65bc\u9ad8\u6ef4\u5ea6\u75c5\u6bd2\u88fd\u5291\uff0c\u91cd\u61f8\u9ad4\u7a4d\u61c9\u9650\u5236\u5728\u767d\u8272\u6c89\u6fb1\u9ad4\u7a4d\u7684\u4e09\u500d\u5de6\u53f3\uff0c\u901a\u5e38\u70ba\u539f\u59cb\u6a23\u54c1\u9ad4\u7a4d\u7684 1\/10 \u81f3 1\/100\u3002\u5982\u679c\u75c5\u6bd2\u61f8\u6d6e\u6db2\u4e2d\u5b58\u5728\u4e0d\u6eb6\u6027\u7269\u8cea\uff0c\u53ef\u900f\u904e\u5728 +4\u00b0C \u4e0b\u4ee5 3,200 x g \u96e2\u5fc3 15 \u5206\u9418\u5c07\u5176\u53bb\u9664\u3002<\/p>\n<p>b) \u907f\u514d\u51b7\u51cd\/\u89e3\u51cd\u5faa\u74b0\u4ee5\u6700\u5927\u9650\u5ea6\u5730\u63d0\u9ad8\u75c5\u6bd2\u6062\u5fa9\u7387\u3002<\/p>\n<p>c) \u75c5\u6bd2\u61f8\u6d6e\u6db2\u4e2d\u5fae\u91cf\u7684PEG\u4e0d\u6703\u5f71\u97ff\u6fc3\u7e2e\u75c5\u6bd2\u7684\u4f7f\u7528\u3002\u5728\u67d0\u4e9b\u60c5\u6cc1\u4e0b\uff0cPEG\u53ef\u80fd\u6703\u63d0\u9ad8\u75c5\u6bd2\u611f\u67d3\u6548\u7387\u3002\u7136\u800c\uff0c\u5982\u679c\u9700\u8981\uff0c\u53ef\u4ee5\u900f\u904e\u4ee5\u4e0b\u6b65\u9a5f\u53bb\u9664\u5fae\u91cf\u7684 PEG\uff1a<\/p>\n<p>\u5c07 1 \u9ad4\u7a4d\u7684\u542b\u6709 4 M KCl \u548c 50 mM Tris-HCl\uff0cpH7.2\uff08\u672a\u63d0\u4f9b\uff09\u7684\u6eb6\u6db2\u52a0\u5165 3 \u9ad4\u7a4d\u7684\u6fc3\u7e2e\u75c5\u6bd2\u61f8\u6d6e\u6db2\u4e2d\u3002\u6216\u8005\uff0c\u5c07\u56fa\u9ad4 KCl \u52a0\u5165\u75c5\u6bd2\u61f8\u6d6e\u6db2\u4e2d\uff0c\u76f4\u5230\u7d42\u6fc3\u5ea6\u70ba 1 M\u3002\u5728\u51b0\u4e0a\u975c\u7f6e 15-30 \u5206\u9418\u3002\u5728 4\u00b0C \u4e0b\u4ee5 12,000 x g \u65cb\u8f49 10 \u5206\u9418\u4ee5\u9664\u53bb\u6c89\u6fb1\u7269\u3002\u5c0f\u5fc3\u6536\u96c6\u75c5\u6bd2\u4e0a\u6e05\u6db2\u3002\u7b49\u5206\u4e26\u5132\u5b58\u65bc -80\u00b0C \u4ee5\u5099\u5c07\u4f86\u4f7f\u7528\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PEG\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u5957\u7d44 | \u8ca8\u865fK904-200\u00a0(5\u500d200\u500b\u53cd\u61c9)\u00a0\/ab102538 PEG Virus [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":10325,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,57],"tags":[3484],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11759"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11759"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11759\/revisions"}],"predecessor-version":[{"id":51924,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11759\/revisions\/51924"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10325"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11759"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11759"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11759"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}