{"id":11753,"date":"2017-07-10T16:41:07","date_gmt":"2017-07-10T08:41:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11753"},"modified":"2023-03-24T13:24:24","modified_gmt":"2023-03-24T05:24:24","slug":"nad-nadh%e5%ae%9a%e9%87%8f%e5%91%88%e8%89%b2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk337","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11753","title":{"rendered":"NAD \/ NADH\u5b9a\u91cf\u5448\u8272\u8a66\u5291\u76d2 | NAD\/NADH Quantitation Colorimetric Kit Biovision\u8ca8\u865fK337 (ABCAM ab65348)"},"content":{"rendered":"

NAD \/ NADH\u5b9a\u91cf\u5448\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK337<\/a>\u00a0\u00a0(ABCAM ab65348)\u00a0<\/span><\/strong><\/p>\n

NAD\/NADH Quantitation Colorimetric Kit- (400 nM – 2000 nM)<\/em><\/strong><\/span><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong> BioVision\u7684NADH \/ NAD\u5b9a\u91cf\u8a66\u5291\u76d2\u70ba\u654f\u611f\u6aa2\u6e2c\u7d30\u80de\u6838\u82f7\u9178NADH\uff0cNAD\u53ca\u5176\u6bd4\u4f8b\u63d0\u4f9b\u4e86\u65b9\u4fbf\u5feb\u901f\u5206\u6790\u7684\u5de5\u5177\u3002 \u8a66\u5291\u76d2\u4e2d\u7684NAD\u5faa\u74b0\u9176\u6df7\u5408\u7269\u5728\u9176\u5faa\u74b0\u53cd\u61c9\u4e2d\u7279\u7570\u6027\u8b58\u5225NADH \/ NAD\u3002 \u4e0d\u9700\u8981\u5f9e\u6a23\u54c1\u4e2d\u7d14\u5316NADH \/ NAD\u3002 \u8a72\u53cd\u61c9\u7279\u7570\u6027\u6aa2\u6e2cNADH\u548cNAD\uff0c\u4f46\u4e0d\u6aa2\u6e2cNADP\u548cNADPH\u3002 \u9176\u5faa\u74b0\u53cd\u61c9\u986f\u8457\u63d0\u9ad8\u4e86\u6aa2\u6e2c\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\u3002 \u901a\u904e\u8207\u6a19\u6e96NADH\u6bd4\u8f03\u53ef\u4ee5\u5bb9\u6613\u5730\u91cf\u5316NADt\uff08NAD\u548cNADH\uff09\u6216NADH\u3002<\/p>\n

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\u529f\u80fd\u7814\u7a76 – NAD\/NADH \u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab65348)<\/strong><\/span><\/div>\n
\n

\u4f7f\u7528 ab65348 \u6e2c\u91cf\u6bd4\u76ee\u9b5a\u808c\u4e2d\u7684 NADH\/NAD+ \u6bd4\u7387\u3002\uff1f\u4f7f\u7528\u7368\u7acb\u7684 t \u6aa2\u9a57\u6bd4\u8f03 (+) HFD \u548c LFD\u3001(*) HFD + TQ \u548c HFD\uff0c\u4ee5\u53ca (#) LFD \u548c LFD + TQ \u6642\u70ba 0.05\u3002\u7d50\u679c\u70ba\u5e73\u5747\u503c\u00b1SEM\uff08\u6bcf\u500b\u6cbb\u7642\u7d44n = 8-10\u96bb\u5c0f\u9f20\uff09\u3002LFD\uff1a\u4f4e\u8102\u80aa\u98f2\u98df\uff0cHFD\uff1a\u9ad8\u8102\u80aa\u98f2\u98df\uff0cTQ\uff1a\u767e\u91cc\u918c\u3002<\/p>\n

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\u529f\u80fd\u7814\u7a76 – NAD\/NADH \u6aa2\u6e2c\u8a66\u5291\u76d2 (ab65348)<\/strong><\/span><\/div>\n
\u5728 K562 ME2 \u6572\u4f4e\u7d30\u80de\uff08pLKO – \u7a7a\u8f09\u9ad4\uff1bshME2-2 \u548c shME2-3 – \u5169\u500b\u9078\u5b9a\u7684\u6572\u4f4e\u514b\u9686\uff09\u4e2d\u6e2c\u91cf NAD\/NADH\u3002\u6578\u64da\u8868\u793a\u70ba\u5e73\u5747\u503c\u00b1\uff1aSD\uff0cn=3\u3002NAD\/NADH \u6bd4\u7387\u6309\u7167\u7522\u54c1\u65b9\u6848\u4e2d\u7684\u63cf\u8ff0\u9032\u884c\u8a08\u7b97\u3002\u00a0\u5716\u7247\u4f86\u81ea Ren JG \u7b49\u4eba\uff1b\u516c\u5171\u79d1\u5b78\u5716\u66f8\u9928\u4e00\u865f\uff0c2010 \u5e74\uff1b5(9): e12520 (DOI:10.1371\/journal.pone.0012520)<\/em><\/div>\n
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\u529f\u80fd\u6e2c\u5b9a\uff1aNAD\/NADH \u6e2c\u5b9a\u8a66\u5291\u76d2 (ab65348)<\/strong><\/span><\/div>\n
\n

NAD \u548c NADH (tNAD) \u6216 NADH \u55ae\u7368\u6e2c\u91cf\u7d30\u80de\u88c2\u89e3\u7269\u30025e6 \u7d30\u80de\u5728 1 mL \u4e2d\u88c2\u89e3\uff0c\u65cb\u8f49\u904e\u6ffe\uff0c\u4e26\u6e2c\u8a66\u7d14\u6216 1\/5\uff08\u91cd\u8907 +\/- SD\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u529f\u80fd\u7814\u7a76 – NAD+\/NADH \u6aa2\u6e2c\u8a66\u5291\u76d2 (ab65348)<\/strong><\/span><\/div>\n
\n

NADH \u6a19\u6e96\u6821\u6e96\u66f2\u7dda\u3002\u9075\u5faa\u7522\u54c1\u65b9\u6848\u4e26\u4f7f\u7528\u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684 NADH \u6a19\u6e96\u54c1\u5c0d NAD\uff08\u83f1\u5f62\uff09\u548c NADH\uff08\u7a7a\u5fc3\u65b9\u584a\uff09\u9032\u884c\u5b9a\u91cf\u3002\u5728\u6b64\u53cd\u61c9\u4e2d\u672a\u6aa2\u6e2c\u5230 NADP\uff08\u4e09\u89d2\u5f62\uff09\u3002\u6a19\u6e96\u66f2\u7dda\u7bc4\u570d\uff1a20-100 pmol\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n

 <\/p>\n<\/div>\n

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\u5546\u54c1\u9700\u7528\u5100\u5668\u6e2c\u91cf<\/span>\u00a0\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(\u6a19\u914d405\u3001450\u3001492\u3001630 nm, \u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247)<\/p>\n

\"RT-6900\"<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n

 <\/p>\n

\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\nSample Type\u6a23\u54c1\u985e\u578b\uff1a\r\n\u52d5\u7269\u7d44\u7e54 (Animal tissues)\uff1a\u809d\u81df (liver)\uff0c\u814e\u81df(kidney)\u7b49\r\n\u7d30\u80de\u57f9\u990a(Cell culture)\uff1a\u8cbc\u58c1(adherent\u00a0)\u6216\u61f8\u6d6e\u7d30\u80de (suspension cells)\r\n\u8840\u6e05(Serum)\u3001\u5c3f\u6db2(Urine)<\/span><\/pre>\n
\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK337-100\u00a0(ABCAM ab65348)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nBioVision\u2019s NADH\/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~2 hours
\n\u2022 Fast and convenient
\n\u2022 Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 NADH\/NAD Extraction Buffer
\n\u2022 NAD Cycling Buffer
\n\u2022 NAD Cycling Enzyme Mix
\n\u2022 NADH Developer
\n\u2022 Stop Solution
\n\u2022 NADH Standard (MW:763)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
 <\/p>\n","protected":false},"excerpt":{"rendered":"
NAD \/ NADH\u5b9a\u91cf\u5448\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK337\u00a0\u00a0(ABCAM ab65348)\u00a0 NAD\/NADH Q […]<\/p>\n","protected":false},"author":3,"featured_media":10325,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[180],"tags":[3485],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11753"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11753"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11753\/revisions"}],"predecessor-version":[{"id":40466,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11753\/revisions\/40466"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10325"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11753"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11753"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11753"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}