{"id":11729,"date":"2017-07-07T16:07:30","date_gmt":"2017-07-07T08:07:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11729"},"modified":"2024-02-02T14:01:30","modified_gmt":"2024-02-02T06:01:30","slug":"%e8%9b%8b%e7%99%bd%e8%b3%aaa-lyophilized-protein-a%e8%b2%a8%e8%99%9f6500b-10","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11729","title":{"rendered":"\u86cb\u767d\u8ceaA (Lyophilized) | Protein A\u8ca8\u865f6500B-10"},"content":{"rendered":"

Protein A (Lyophilized) <\/strong>\u8ca8\u865f<\/strong>6500B-10<\/em><\/strong><\/a><\/span><\/p>\n

\"biovision01\"<\/a><\/p>\n

Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies<\/em><\/strong><\/p>\n

Protein A\u662f\u4e00\u7a2e\u7d93\u907a\u50b3\u5de5\u7a0b\u6539\u9020\u7684\u86cb\u767d\u8cea\uff0c\u5176\u5728N\u672b\u7aef\u542b\u6709\u86cb\u767d\u8ceaA\u548c6x His\u6a19\u7c64\u76845\u500bIg\u7d50\u5408\u5340\u3002 \u7d30\u80de\u58c1\u7d50\u5408\u5340\uff0c\u767d\u86cb\u767d\u7d50\u5408\u5340\u548c\u5176\u4ed6\u975e\u7279\u7570\u6027\u7d50\u5408\u5340\u5df2\u5f9e\u91cd\u7d44\u86cb\u767dA\u4e2d\u6d88\u9664\uff0c\u4ee5\u78ba\u4fdd\u6700\u5927\u7279\u7570\u6027IgG\u7d50\u5408\u3002 6x His\u6a19\u7c64\u53ef\u7528\u65bc\u89aa\u548c\u7d14\u5316\u6216\u4f7f\u7528\u6297His\u6a19\u7c64\u6297\u9ad4\u9032\u884c\u86cb\u767dA\u6aa2\u6e2c\u3002Protein A\u662f\u7d14\u5316\u591a\u514b\u9686\u6216\u55ae\u514b\u9686IgG\u6297\u9ad4\u7684\u7406\u60f3\u9078\u64c7\u3002 Protein A\u8207\u5927\u591a\u6578\u4eba\u548c\u5c0f\u9f20IgG\u4e9e\u985e\uff08\u4f8b\u5982\u4ebaIgG1\uff0cIgG2\uff0cIgG4;\u5c0f\u9f20IgG1\uff0cIgG2a\uff0cIgG2b\uff0cIgG3\uff09\u7d50\u5408\u3002 \u5b83\u9084\u7d50\u5408\u4f86\u81ea\u725b\uff0c\u8c5a\u9f20\uff0c\u5009\u9f20\uff0c\u99ac\uff0c\u8c6c\u548c\u5154\u7684\u7e3dIgG\u3002 \u86cb\u767dA\u5c0d\u96de\uff0c\u5c71\u7f8a\uff0c\u5927\u9f20\u548c\u7dbf\u7f8a\u5e7e\u4e4e\u6c92\u6709\u89aa\u548c\u529b\u3002<\/p>\n

\"6500b\"<\/a><\/p>\n

The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/th>\nProtein A<\/td>\n<\/tr>\n
Gene ID<\/th>\n3919448<\/td>\n<\/tr>\n
Accession #<\/th>\nYP_498670<\/td>\n<\/tr>\n
Source<\/th>\nE. coli<\/td>\n<\/tr>\n
Appearance<\/th>\nLyophilized<\/td>\n<\/tr>\n
Physical Form Description<\/th>\nLyophilized powder<\/td>\n<\/tr>\n
Molecular Weight<\/th>\n35.5 kDa<\/td>\n<\/tr>\n
Purity by SDS-PAGE<\/th>\n\u226597%<\/td>\n<\/tr>\n
Purity by<\/th>\n\u226597%<\/td>\n<\/tr>\n
Reconstitution Instructions<\/th>\nReconstitute in H\u2082O<\/td>\n<\/tr>\n
Handling<\/th>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/th>\nFor Research Use Only! Not to be used in humans<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

Nagatsuka, Takehiro et al. (2017) Preparation of silicon nitride biochips for reflectometric interference spectroscopic (RIfS) analysis of biological toxins and E. coli O157:H7 strain,\u00a0Sensors and Actuators B: Chemical, 2017 Jul;246:937-942<\/a><\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"ELISA<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Protein A (Lyophilized) \u8ca8\u865f6500B-10 Genetically engineer […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3491],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11729"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11729"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11729\/revisions"}],"predecessor-version":[{"id":48593,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11729\/revisions\/48593"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11729"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11729"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11729"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}