{"id":11622,"date":"2017-07-05T17:11:21","date_gmt":"2017-07-05T09:11:21","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11622"},"modified":"2022-07-21T15:31:22","modified_gmt":"2022-07-21T07:31:22","slug":"ighm%e5%8d%b3%e7%94%a8%e5%9e%8b%e6%85%a2%e7%97%85%e6%af%92-sirnashrnarnai-lentivirus-human-pooled-%e8%b2%a8%e8%99%9fiv010442","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11622","title":{"rendered":"IGHM\u5373\u7528\u578b\u6162\u75c5\u6bd2 | siRNA\/shRNA\/RNAi Lentivirus (Human) pooled \u8ca8\u865fiV010442"},"content":{"rendered":"

IGHM \u00a0siRNA\/shRNA\/RNAi Lentivirus (Human) pooled \u5373\u7528\u578b\u6162\u75c5\u6bd2<\/strong><\/a><\/span> |<\/strong> 2×200\u00b5l>107<\/sup>\u00a0IU\/ml\u8ca8\u865f<\/strong>iV010442<\/p>\n

Abmgood iLenti RNAi\u8868\u9054\u7cfb\u7d71\uff0c\u53ef\u7d93\u7531\u8f49\u67d3\u6216\u6162\u75c5\u6bd2\u611f\u67d3\u6709\u6548\u8868\u9054\u4efb\u4f55siRNA\u3002 \u8a72\u7cfb\u7d71\u57fa\u65bc\u7368\u7279\u7684convergen\u555f\u52d5\u5b50\u8a2d\u8a08\uff0c\u63d0\u9ad8\u57fa\u56e0knockdown\u6548\u7387\uff0c\u4e0d\u9700\u8981\u55ae\u500b\u555f\u52d5\u5b50\u8f09\u9ad4\u4e2d\u4f7f\u7528\u4e4bhair-pin loop\u00a0\u9aee\u593e\u74b0\u7d50\u69cb\u3002 iLenti\u2122siRNA\u7684\u512a\u9ede<\/p>\n

    \n
  1. siRNA\u6162\u75c5\u6bd2\u8f09\u9ad4\u53ef\u61c9\u7528transfection\u8f49\u67d3\u6216viral infection\u75c5\u6bd2\u611f\u67d3\u3002<\/li>\n
  2. \u7a69\u5b9a\u548c\u9ad8\u7522\u91cf\u7684plasmids; \u4e0d\u9700\u8981\u7279\u6b8a\u7684competent cells\u3002<\/li>\n
  3. 27-29 bp\u5be1\u6838\u82f7\u9178, \u6bd4\u50b3\u7d71\u768419\u621621 bp\u5be1\u6838\u82f7\u9178\u66f4\u6709\u6548\u7684<\/li>\n
  4. Convergent\u555f\u52d5\u5b50\u907f\u514dhairpin loop\u9aee\u593e\u74b0\u7d50\u69cb<\/li>\n
  5. GFP reporter\u76e3\u6e2c\u8f49\u67d3\u6216\u75c5\u6bd2\u611f\u67d3\u3002<\/li>\n
  6. \u6240\u6709\u69cb\u5efa\u9ad4\u5747\u53ef\u7528\u8f09\u9ad4\u6216\u75c5\u6bd2\u5f62\u5f0f\u63d0\u4f9b\uff0c\u6216\u63d0\u4f9b\u5408\u4f75\u75c5\u6bd2\uff0c\u7528\u65bc\u6539\u826f\u57fa\u56e0\u6572\u9664\u7684\u6548\u80fd<\/li>\n<\/ol>\n

    \"\"<\/p>\n

    \u5176\u5b83\u75c5\u6bd2\u6db2\u5546\u54c1\u00a0A4GALT<\/a>\u00a0|IGLC2<\/a>\u00a0|\u00a0IGHM \u00a0<\/a>\u00a0|\u00a0IGHD<\/a>\u00a0|\u00a0BRCA1\u00a0<\/a>\u00a0|\u00a0BRCA2\u00a0<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
    Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
    Accession Number<\/strong><\/td>\nNG_001019<\/a><\/td>\n<\/tr>\n
    Vector<\/strong><\/td>\npiLenti-siRNA-GFP<\/a><\/td>\n<\/tr>\n
    Target Sequence<\/strong><\/td>\nSequence available upon placing order<\/td>\n<\/tr>\n
    Mammalian Selection<\/strong><\/td>\nPuromycin<\/td>\n<\/tr>\n
    Format<\/strong><\/td>\nVirus<\/td>\n<\/tr>\n
    Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
    Storage<\/strong><\/td>\nLentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80\u00b0C in small aliquots to avoid repeated freeze-thaw cycles.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u5176\u5b83\u5546\u54c1 CRISPR\u6aa2\u6e2c\u5957\u7d44<\/a> | CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de\u682a<\/a> | CASPASE9 (D10A) NLS<\/a><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    IGHM \u00a0siRNA\/shRNA\/RNAi Lentivirus (Human) pooled \u5373\u7528\u578b\u6162\u75c5\u6bd2 […]<\/p>\n","protected":false},"author":3,"featured_media":10852,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[456],"tags":[3510],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11622"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11622"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11622\/revisions"}],"predecessor-version":[{"id":11650,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11622\/revisions\/11650"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10852"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11622"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11622"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11622"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}