{"id":11317,"date":"2017-06-29T11:47:22","date_gmt":"2017-06-29T03:47:22","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11317"},"modified":"2025-09-26T18:14:32","modified_gmt":"2025-09-26T10:14:32","slug":"%e7%be%a5%e8%84%af%e6%b0%a8%e9%85%b8%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-hydroxyproline-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk555-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11317","title":{"rendered":"\u7fa5\u812f\u6c28\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2 | Hydroxyproline Colorimetric Assay Kit\u8ca8\u865fK555-100 (abcam ab222941)"},"content":{"rendered":"

\u5fc3\u8840\u7ba1\u75be\u75c5\u76f8\u95dc\u7814\u7a76-\u814e\u640d\u50b7\/\u814e\u75c5<\/strong><\/p>\n

\u5fc3\u8840\u7ba1\u75be\u75c5\uff08CVD\uff09\u662f\u5168\u7403\u767c\u75c5\u548c\u6b7b\u4ea1\u7684\u4e3b\u8981\u539f\u56e0\u3002 CVD\u7684\u57fa\u790e\u5305\u62ec\u5c0d\u6700\u7d42\u963b\u7919\u8840\u6db2\u6d41\u52d5\u7684\u5fc3\u81df\u548c\u8840\u7ba1\u7684\u640d\u5bb3\u3002 \u9019\u7a2e\u5ee3\u6cdb\u7684\u75be\u75c5\u5305\u62ec\u51a0\u72c0\u52d5\u8108\u75be\u75c5\uff08\u4f8b\u5982\u5fc3\u808c\u6897\u585e\u6216\u5fc3\u81df\u75c5\u767c\u4f5c\uff0c\u5fc3\u7d5e\u75db\u7b49\uff09\uff0c\u5fc3\u5f8b\u5931\u5e38\uff0c\u4e2d\u98a8\uff0c\u5148\u5929\u6027\u5fc3\u81df\u75c5\u548c\u814e\/\u8840\u7ba1\u75be\u75c5\u3002 CVD\u7684\u591a\u91cd\u5371\u96aa\u56e0\u7d20\u5305\u62ec\u5e74\u9f61\uff0c\u98f2\u98df\uff0c\u751f\u6d3b\u7fd2\u6163\u8b8a\u5316\uff0c\u907a\u50b3\u50be\u5411\u548c\u9ad8\u8840\u58d3\u5bb6\u65cf\u53f2\uff0c\u7cd6\u5c3f\u75c5\u548c\/\u6216\u80a5\u80d6\u3002 BioVision\u63d0\u4f9b\u5168\u9762\u7684\u7522\u54c1\u7d44\u5408\uff0c\u5305\u62ec\u6e2c\u5b9a\u8a66\u5291\u76d2\uff0c\u91cd\u7d44\u86cb\u767d\uff0c\u6297\u9ad4\u548c\u751f\u7269\u5316\u5b78\u54c1\uff0c\u4ee5\u4fc3\u9032\u5fc3\u8840\u7ba1\u751f\u7269\u5b78\uff0c\u85e5\u7269\u958b\u767c\u548c\u751f\u7269\u6a19\u8a8c\u7269\u767c\u73fe\u7814\u7a76\u3002<\/p>\n

\u7fa5\u812f\u6c28\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2 |<\/strong>\u8ca8\u865f<\/em><\/strong>K555-100<\/strong><\/a>\u00a0(abcam ab222941)\u00a0<\/span><\/p>\n

Hydroxyproline Colorimetric Assay Kit<\/p>\n

\u539f\u7406\u4ecb\u7d39<\/strong>:<\/strong> \u7fa5\u812f\u6c28\u9178\uff084-\u7fa5\u812f\u6c28\u9178\uff09\u662f\u5e38\u898b\u7684\u975e\u86cb\u767d\u539f\u6027\u6c28\u57fa\u9178\u3002\u50c5\u5728\u54fa\u4e73\u52d5\u7269\u7684\u81a0\u539f\u86cb\u767d\u548c\u5f48\u6027\u86cb\u767d\u4e2d\u767c\u73fe\uff0c\u4f46\u5728\u690d\u7269\u4e2d\u5b58\u5728\u8a31\u591a\u5176\u5b83\u86cb\u767d\u8cea\u3002\u7fa5\u812f\u6c28\u9178\u50c5\u4f5c\u70ba\u80bd\u93c8\u4e2d\u7684\u7ffb\u8b6f\u5f8c\u4fee\u98fe\u5f62\u6210\uff0c\u812f\u6c28\u9178\u7fa5\u5316\u9176\u4e0d\u6703\u4f7f\u6e38\u96e2\u812f\u6c28\u9178\u7fa5\u57fa\u5316\u3002\u7d44\u7e54\u6c34\u89e3\u7269\u4e2d\u7684\u7fa5\u812f\u6c28\u9178\u662f\u76f4\u63a5\u6e2c\u91cf\u81a0\u539f\u86cb\u767d\u6216\u660e\u81a0\u7684\u91cf\u3002\u64da\u4fe1\u5404\u7a2e\u75be\u75c5\u72c0\u614b\u6703\u5f71\u97ff\u81a0\u539f\u5468\u8f49\uff0c\u4e26\u53ef\u5f15\u8d77\u8840\u6e05\u6216\u5c3f\u6db2\u7fa5\u812f\u6c28\u9178\u5347\u9ad8\u3002\u9019\u4e9b\u75c5\u75c7\u7bc4\u570d\u5f9e\u816b\u7624\uff0c\u708e\u75c7\uff0c\u814e\u6216\u9aa8\u75be\u75c5\u5230\u5167\u5206\u6ccc\u548c\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5\u3002 BioVision\u7684\u7fa5\u812f\u6c28\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2\u65e8\u5728\u6e2c\u91cf\u7d44\u7e54\u6216\u86cb\u767d\u8cea\/\u80bd\u6c34\u89e3\u7522\u7269\u4e2d\u7684\u7fa5\u812f\u6c28\u9178\u3002\u5982\u679c\u5df2\u7d93\u7d93\u904e\u4e86\u5148\u524d\u7684\u7d14\u5316\u904e\u7a0b\uff0c\u5b83\u53ef\u7528\u65bc\u6e2c\u91cf\u5176\u4ed6\u751f\u7269\u6a23\u54c1\u5982\u8840\u6e05\u6216\u5c3f\u6db2\u4e2d\u7684\u7fa5\u812f\u6c28\u9178\u3002\u9019\u662f\u4e00\u7a2e\u5bb9\u6613\uff0c\u65b9\u4fbf\u7684\u65b9\u6cd5\uff0c\u5176\u5c0e\u81f4\u5728560nm\u8655\u5177\u6709\u6700\u5927\u5438\u5149\u5ea6\u7684\u8272\u539f\u9ad4\u3002\u8a72\u6e2c\u5b9a\u57280.1-2\u5fae\u514b\u7bc4\u570d\u5167\u6709\u7528\u3002<\/p>\n

\"\"<\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK555-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (560 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe assay is useful over the range of 0.1-2 \u03bcg.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than ~100 minutes
\n\u2022 Convenient and easy to use<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Oxidation Buffer
\n\u2022 Chloramine T Concentrate
\n\u2022 Perchloric acid\/Isopropanol Solution
\n\u2022 DMAB Concentrate (in DMSO)
\n\u2022 Hydroxyproline Standard (1 mg\/ml)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1\u00a0AHU-377<\/a>\u00a0|\u00a0CHIR-98014<\/a>\u00a0|\u00a0C-\u53cd\u61c9\u86cb\u767d\uff08CRP\uff09ELISA\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u00a0AT 56<\/a>\u00a0|\u00a0\u767d\u86cb\u767d\u5c0d\u808c\u9150\u6bd4\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a> | \u00a0\u8840\u7d05\u86cb\u767d\u6e2c\u5b9a<\/a>\u00a0|\u7fa5\u812f\u6c28\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/p>\n

\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

\n
1. Cui, Huachun et al. (2017) miR-34a promotes fibrosis in aged lungs by inducing alveolarepithelial dysfunctions,\u00a0Am J Physiol Lung Cell Mol Physiol. 2017 Mar 1;312(3):L415-L424.<\/a><\/div>\n<\/div>\n
\n
2. Higuchi, Sei et al. (2016) EP4 Receptor\u2013Associated Protein in Macrophages Protects against Bleomycin-Induced Pulmonary Inflammation in Mice,\u00a0J Immunol. 2016 Dec 1;197(11):4436-4443.<\/a><\/div>\n<\/div>\n
\n
3. Al-Eisa, Einas S. et al. (2016) Correlation between vitamin D levels and muscle fatigue risk factors based on physical activity in healthy older adults,\u00a0Clin Interv Aging. 2016 May 4;11:513-22.<\/a><\/div>\n<\/div>\n
\n
4. Khong, Nicholas M.H. et al. (2016) Nutritional composition and total collagen content of three commercially important edible jellyfish,\u00a0Food Chem. 2016 Apr 1;196:953-60.<\/a><\/div>\n<\/div>\n
\n
5. Chagnon et al., Smart imaging of acute lung injury: exploration of myeloperoxidase activity using in vivo endoscopic confocal fluorescence microscopy.\u00a0Am J Physiol Lung Cell Mol Physiol,\u00a0Sep 2015; 309: L543 – L551.<\/a><\/div>\n<\/div>\n

\u8a66\u5291\u88fd\u5099\uff1a<\/strong><\/p>\n

Chloramine T Reagent\u6c2f\u80faT\u8a66\u5291\uff1a\u5c0d\u65bc\u6bcf\u500b\u5f85\u5206\u6790\u7684\u5b54\uff0c\u52a0\u51656\u03bcl\u6c2f\u80faT\u6fc3\u7e2e\u6db2\u523094\u03bcl\u6c27\u5316\u7de9\u885d\u6db2\u4e2d\u4e26\u6df7\u5408\u597d\u3002DMAB\u8a66\u5291\uff1a\u5c0d\u65bc\u6bcf\u500b\u8981\u5206\u6790\u7684\u5b54\uff0c\u52a0\u516550\u03bcl\u7684DMAB\u6fc3\u7e2e\u6db2\u523050\u03bcl\u7684\u9ad8\u6c2f\u9178\/\u7570\u4e19\u9187\u6eb6\u6db2\u4e2d\uff0c\u62cc\u52fb\u3002 \u4fdd\u6301\u51b0\uff0c\u907f\u5149\u3002<\/p>\n

\u6ce8\u610f\uff1a\u8a66\u5291\u6fc3\u7e2e\u7269\u4f9b\u61c9\u7a69\u5b9a\u3002 \u4e00\u65e6\u6fc3\u7e2e\u6db2\u88ab\u7a00\u91cb\u5230\u5de5\u4f5c\u6fc3\u5ea6\uff0c2-3\u500b\u5c0f\u6642\u5167\u4f7f\u7528\u5b8c\u3002\u6240\u4ee5\u53ea\u9700\u8981\u88fd\u4f5c\u76e1\u53ef\u80fd\u591a\u7684\u8a66\u5291\u4ee5\u4fbf\u91cf\u5316\u6a23\u54c1\u6578\u91cf\u548c\u6a19\u6e96\u3002<\/p>\n

\u6a23\u54c1\u88fd\u5099\uff1a<\/strong><\/p>\n

\u7d44\u7e54\u6216\u86cb\u767d\u8cea\/\u80bd\u6a23\u54c1\u5982\u80ba\u7d44\u7e54\u61c9\u5728dH2O\u4e2d\u52fb\u6f3f\uff0c\u4f7f\u7528100\u03bclH2O\/10\u6beb\u514b\u7684\u7d44\u7e54\u3002\u5411100\u03bcl\u6a23\u54c1\u52fb\u6f3f\u4e2d\u52a0\u5165100\u03bcl\u6fc3HCl\uff08\u7d0412N\uff0c\u672a\u63d0\u4f9b\uff09\uff0c\u5728\u58d3\u529b\u4e0b\uff0c\u805a\u56db\u6c1f\u4e59\u70ef\u52a0\u84cb\u5c0f\u74f6\u4e26\u5728120\u2103\u6c34\u89e33\u5c0f\u6642\u3002\u5728\u5c3f\u6db2\u7684\u60c5\u6cc1\u4e0b\uff0c\u7528\u76f8\u540c\u9ad4\u7a4d\u7684\u6fc3\u7e2e\u6c34\u6a23\u5c3f\u6db2HCl\uff08\u7d0412N;\u5373100\u03bcl\u5c3f\u6db2+100\u03bclHCl\uff09\u4e2d\uff0c\u5728\u58d3\u529b\u5bc6\u5c01\u7684\u805a\u56db\u6c1f\u4e59\u70ef\u52a0\u84cb\u7684\u5c0f\u74f6\u4e2d\u3002\u5728120\u00b0C\u6c34\u89e33\u5c0f\u6642\uff08\u64cd\u4f5c\u624b\u518a\u8a3bc\uff09\u3002\u6f84\u6e05\u5c3f\u6a23\u7528\u6d3b\u6027\u70ad\u52a0\u6d3b\u6027\u70ad4\u6beb\u514b\u3002\u6e26\u65cb\u4e26\u4ee510000\u00d7g\u96e2\u5fc33\u5206\u9418\u3002\u53bb\u9664\u6c89\u6fb1\u548c\u6d3b\u6027\u70ad\u3002\u5982\u679c\u9700\u8981\u91cd\u8907\u3002\u8f49\u79fb10\u03bcL\u7684\u6bcf\u500b\u6c34\u89e3\u6a23\u54c1\u523096\u5b54\u677f\uff0c\u84b8\u767c\u81f3\u5728\u771f\u7a7a\u4e0b\u4e7e\u71e5\u3002\u5c0d\u65bc\u672a\u77e5\u7684\u6a23\u54c1\uff0c\u6211\u5011\u5efa\u8b70\u9032\u884c\u4e00\u500b\u8a66\u9a57\u5be6\u9a57\u548c\u6e2c\u8a66\u4e0d\u540c\u7684\u6a23\u54c1\u7a00\u91cb\u5ea6\uff0c\u4ee5\u78ba\u4fdd\u8b80\u6578\u5728\u6a19\u6e96\u66f2\u7dda\u7bc4\u570d\u5167\u3002\u5167\u6e90\u6027\u5316\u5408\u7269\u53ef\u80fd\u5e72\u64fe\u53cd\u61c9\u3002\u70ba\u78ba\u4fdd\u6e2c\u8a66\u6a23\u54c1\u4e2d\u7fa5\u812f\u6c28\u9178\u7684\u6e96\u78ba\u6e2c\u5b9a\uff0c\u6211\u5011\u5efa\u8b70\u4f7f\u7528\u5df2\u77e5\u91cf\u7684\u6a19\u6e96\u54c1\uff080.4\u03bcg\uff09\u52a0\u6a19\u6a23\u54c1\u3002\u5c0d\u65bc\u6a23\u54c1\u6c34\u89e3\uff0c\u805a\u4e19\u70ef\u5c0f\u74f6\u7522\u751f\u6700\u597d\u7684\u7d50\u679c\u3002\u6211\u5011\u63a8\u85a6Biovision\u7684\u805a\u4e19\u70ef\u74f6\u548c\u84cb\u5b50(M1353\u548cM1352\uff09\u3002<\/p>\n

\u6a19\u6e96\u66f2\u7dda\u88fd\u5099\uff1a<\/strong><\/p>\n

\u5c0710\u03bcl 1mg \/ ml\u6a19\u6e96\u54c1\u52a0\u5165\u523090\u03bcl\u7684\u6a19\u6e96\u66f2\u7dda\u4e2d\uff0c\u5c07\u7fa5\u812f\u6c28\u9178\u6a19\u6e96\u54c1\u7a00\u91cb\u81f30.1mg \/ ml dH2O\u62cc\u52fb\u3002\u6bcf\u5b54\u4e2d\u52a0\u51650,2,4,6,8,10\u03bcl\u7522\u751f0.2,0.4,0.6,0.8\u548c1\u03bcg\/\u5b54\u7684\u7fa5\u812f\u6c28\u9178\u6a19\u6e96\u54c1\u3002<\/p>\n

3.\u53cd\u61c9\uff1a\u5411\u6bcf\u500b\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e2d\u52a0\u5165100\u03bcl\u6c2f\u80faT\u8a66\u5291\uff0c\u4e26\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b25\u5206\u9418\u3002\u52a0\u5411\u6bcf\u500b\u5b54\u4e2d\u52a0\u5165100\u03bcl\u7684DMAB\u8a66\u5291\u4e26\u5b75\u80b290\u5206\u9418\u3002\u572860\u2103\u4e0b\u3002<\/p>\n

4.\u6e2c\u91cf\uff1a\u5728\u9176\u6a19\u5100\u4e0a\u6e2c\u91cf560nm\u8655\u7684\u5438\u5149\u5ea6\u3002<\/p>\n

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