{"id":11056,"date":"2017-06-26T14:32:45","date_gmt":"2017-06-26T06:32:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11056"},"modified":"2022-07-21T17:34:13","modified_gmt":"2022-07-21T09:34:13","slug":"pgb-caspase-3-sirna-%e8%bc%89%e9%ab%94-%e8%b2%a8%e8%99%9f1143-5","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=11056","title":{"rendered":"pGB Caspase-3 siRNA \u8f09\u9ad4 | \u8ca8\u865f1143-5"},"content":{"rendered":"

pGB Caspase-3 siRNA Vector <\/strong>\u8ca8\u865f<\/strong>1143-5<\/strong><\/a><\/span><\/p>\n

pGB expression vector to supress Caspase 3 activity in transfected cells<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39: pGB\u8868\u9054\u8f09\u9ad4\u6291\u5236\u8f49\u67d3\u7d30\u80de\u4e2d\u7684\u534a\u80f1\u5929\u51ac\u91763\u6d3b\u6027\u3002pGB\u8868\u9054\u8f09\u9ad4\u542b\u6709\u4ebaU6 RNA\u805a\u5408\u9176III\u555f\u52d5\u5b50\uff0c\u5176\u5f15\u5c0e\u8a31\u591a\u7d30\u80de\u4e2d\u77edRNA\u8f49\u9304\u672c\u7684\u7d44\u6210\u578b\uff0c\u9ad8\u6c34\u5e73\u8868\u9054\u3002 \u6bcf\u500b\u8f09\u9ad4\u9084\u542b\u6709\u65b0\u9ef4\u7d20\/\u5361\u90a3\u9ef4\u7d20\u6297\u6027\u57fa\u56e0\uff0c\u4ee5\u63d0\u4f9b\u7d30\u83cc\u4e2d\u7684\u5361\u90a3\u9ef4\u7d20\u6297\u6027\u548c\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u4e2d\u7684G418\u6297\u6027\u3002 pGB\u5411\u91cf\u7528\u65bc\u514b\u9686\u60a8\u81ea\u5df1\u7684\u63d2\u5165\u3002 \u8f09\u9ad4\u5305\u542b\u5169\u500b\u7368\u7279\u7684\u9650\u5236\u6027\u4f4d\u9ede\uff0cBamH I\u548cXba I\u7528\u65bc\u5b9a\u5411\u514b\u9686\u3002 pGB\u9670\u6027\u5c0d\u7167\u8f09\u9ad4\u542b\u6709\u8207\u4eba\uff0c\u5c0f\u9f20\u548c\u5927\u9f20\u4e2d\u8868\u9054\u7684\u54fa\u4e73\u52d5\u7269\u57fa\u56e0\u4e0d\u5177\u6709\u986f\u8457\u540c\u6e90\u6027\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u53ef\u7528\u4f5cpGB-Caspase siRNA\u8f09\u9ad4\u7684\u9670\u6027\u5c0d\u7167\u3002 pGB\u80f1\u5929\u86cb\u767d\u9176siRNA\u8f09\u9ad4\u542b\u6709\u8a2d\u8a08\u7528\u65bc\u55ae\u7368\u6291\u5236\u6bcf\u7a2e\u534a\u80f1\u5929\u51ac\u9176\u8868\u9054\u7684siRNA\u63d2\u5165\u7247\u6bb5\u3002<\/p>\n\n\n\n
Highlights<\/strong><\/td>\n\u2022 (GeneBlocker\u2122 Caspase-3 siRNA Vector, pGB-Casp-3)
\n\u2022 Application- The pGB siRNA vectors can be transfected into mammalian cells using Lipofectamine (Invitrogen). For transient transfection, cells can be analyzed in 24-96 hours following transfections, by Western blot analysis or other detection means. For stable transfections, cells can be selected in G418 selection medium to obtain stable cell lines with the specific gene blocked.The pGB cloning vector is designed for cloning your own siRNA insert.
\n\u2022 Features and Positions:
\nHuman U6 Promoter: 1-256
\nMultiple cloning Site: 259-285
\n3\u2019 Primer: 398-426 (GAAGCATTTATCAGGGTTATTGTCTCATG)
\nSV40 Promoter: 470-808
\nNeomycin\/Kanamycin Resistance ORF: 843-1634
\n5\u2019 Primer: 2789-2813 (CGTCGATTTTTGTGATGCTCGTCAG)
\npUC Origin of Replication: 2222-3003<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>\u76f8\u95dc\u5546\u54c1\u00a0<\/span>Z-DEVD-FMK<\/u><\/a> | Caspase-3 (Active) <\/u>\u6297\u9ad4<\/u><\/a> |\u00a0Caspase-3 siRNA <\/u>\u8f09\u9ad4<\/u><\/a> | DEVD-pNA<\/u>\u00a0<\/a> | \u00a0Caspase-3<\/u>\u6291\u5236\u5291\u85e5\u7269\u7be9\u9078<\/u><\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

pGB Caspase-3 siRNA Vector \u8ca8\u865f1143-5 pGB expression vect […]<\/p>\n","protected":false},"author":3,"featured_media":9204,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[128],"tags":[3583],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11056"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11056"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11056\/revisions"}],"predecessor-version":[{"id":11066,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11056\/revisions\/11066"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9204"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11056"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11056"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11056"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}