{"id":10859,"date":"2017-06-21T11:10:10","date_gmt":"2017-06-21T03:10:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10859"},"modified":"2022-07-22T09:50:32","modified_gmt":"2022-07-22T01:50:32","slug":"sumo%e8%9b%8b%e7%99%bd%e9%85%b61-small-ubiquitin-like-modifiers-protease-1%ef%bc%88his%ef%bc%89%e9%85%b5%e6%af%8d%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9f7868","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=10859","title":{"rendered":"His-SUMO\u86cb\u767d\u91761| Small Ubiquitin-like MOdifiers Protease 1\uff08His\uff09\u9175\u6bcd\u91cd\u7d44\u86cb\u767d \u8ca8\u865f7868"},"content":{"rendered":"

EZCut TM<\/sup> SUMO<\/strong>\u86cb\u767d\u9176<\/strong>1<\/strong>\uff08<\/strong>His<\/strong>\u6a19\u7c64\uff09\uff0c\u9175\u6bcd\u91cd\u7d44\u86cb\u767d<\/strong>\u00a0| \u8ca8\u865f7868<\/strong><\/a><\/span><\/p>\n

SUMO<\/strong>\uff08\u5c0f\u6cdb\u7d20\u6a23MOI\uff09\u86cb\u767d\u91761<\/strong>\u4ecb\u7d39<\/strong>:<\/strong> SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae)\u4f86\u81ea\u91c0\u9152\u9175\u6bcd\u7684Ulp1\uff0cUbl\u7279\u7570\u6027\u86cb\u767d\u91761\uff09\u662f\u9ad8\u6d3b\u6027\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176\uff0c\u5177\u9ad8\u5ea6\u7279\u7570\u6027\uff0c\u8b58\u5225\u6cdb\u7d20\u6a23\uff08UBL\uff09\u86cb\u767d\uff08SUMO\uff08Smt3\uff09\uff09\u7684\u4e09\u7d1a\u7d50\u69cb\uff0c\u800c\u4e0d\u662f\u5176\u6c28\u57fa\u9178\u5e8f\u5217\u3002\u4f5c\u70baN\u672b\u7aef\u878d\u5408\u4f34\u4fb6\u7684SUMO\u878d\u5408\u6a19\u7c64\u5df2\u88ab\u8b49\u660e\u53ef\u589e\u5f37\u539f\u6838\u548c\u771f\u6838\u8868\u9054\u7cfb\u7d71\u4e2d\u7684\u529f\u80fd\u6027\u86cb\u767d\u8cea\u7522\u751f\uff0c\u986f\u8457\u63d0\u9ad8\u4e86\u86cb\u767d\u8cea\u7684\u7a69\u5b9a\u6027\u548c\u6eb6\u89e3\u5ea6\u3002\u542b\u6709N-\u672b\u7aefHis\u6a19\u7c64\u7684\u91cd\u7d44EZCut TM SUMO\u86cb\u767d\u91761\uff0c\u5728\u5927\u8178\u687f\u83cc\u4e2d\u8868\u9054\u4e26\u901a\u904e\u5c08\u6709\u7684\u8272\u8b5c\u6280\u8853\u7d14\u5316\u3002\u53ef\u7528\u65bc\u5f9e\u91cd\u7d44SUMO\u878d\u5408\u86cb\u767d\u5207\u5272SUMO\u86cb\u767d\u6a19\u7c64\u3002\u5207\u5272\u7684\u6700\u4f73\u6eab\u5ea6\u70ba30\u2103; \u7136\u800c\uff0c\u9176\u5728\u5bec\u7684\u6eab\u5ea6\u548cpH\u7bc4\u570d\u5167\u662f\u6709\u6d3b\u6027\u7684\u3002\u88c2\u89e3\u53cd\u61c9\u5b8c\u6210\u5f8c\uff0c\u4f7f\u7528Ni\u87af\u5408\u6a39\u8102\uff08Cat\u3002\uff036562-1,10,100,500\uff09\u7684\u89aa\u548c\u5c64\u6790\u53ef\u4ee5\u5bb9\u6613\u5730\u5f9e\u53cd\u61c9\u4e2d\u9664\u53bb\u86cb\u767d\u9176\u3002 The EZCut\u2122 SUMO Protease 1 (His-tagged) has an activity of \u2265 1 X 106 units\/mg.<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nUlp1 peptidase, SUMO Protease<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nULP1<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n856087<\/td>\n<\/tr>\n
Accession #<\/strong><\/td>\nQ02724<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nE. coli<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\n0.1 mg\/ml (100 U\/\u00b5l) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n28.7 kDa (403-621 aa + N-terminal Poly-His tag).<\/td>\n<\/tr>\n
Purity by SDS-PAGE<\/strong><\/td>\n\u226590%<\/td>\n<\/tr>\n
Biological Activity<\/strong><\/td>\nThe EZCut\u2122 SUMO Protease 1 (His-tagged) has an activity of \u2265 1 X 106 units\/mg.<\/td>\n<\/tr>\n
Amino Acid Sequence<\/strong><\/td>\n403-621 aa + N-terminal Poly-His tag<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/strong><\/span> SUMO\u86cb\u767d\u91761\uff08His\u6a19\u7c64\uff09<\/a> | SUMO\u86cb\u767d\u91761\uff08GST-tagged\uff09<\/a>| SUMO\u86cb\u767d\u91761\u74ca\u8102\u7cd6\u73e0<\/a> | SUMO-GFP<\/a> | \u91cd\u7d44SUMO2-Rhodamine<\/a> | \u91cd\u7d44SUMO1-AMC\u00a0<\/a><\/strong><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

EZCut TM SUMO\u86cb\u767d\u91761\uff08His\u6a19\u7c64\uff09\uff0c\u9175\u6bcd\u91cd\u7d44\u86cb\u767d\u00a0| \u8ca8\u865f7868 SUMO\uff08\u5c0f\u6cdb\u7d20\u6a23MOI\uff09\u86cb […]<\/p>\n","protected":false},"author":3,"featured_media":8690,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[3625],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10859"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10859"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10859\/revisions"}],"predecessor-version":[{"id":10889,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10859\/revisions\/10889"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8690"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10859"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10859"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10859"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}