{"id":10739,"date":"2017-06-14T15:40:09","date_gmt":"2017-06-14T07:40:09","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10739"},"modified":"2022-07-22T09:53:43","modified_gmt":"2022-07-22T01:53:43","slug":"a1bg-sgrna-crisprcas9-%e7%97%85%e6%af%92%e8%bc%89%e9%ab%94-all-in-one-lentivector-rat-target-3-%e8%b2%a8%e8%99%9f-t3251","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=10739","title":{"rendered":"A1bg sgRNA CRISPR\/Cas9 \u75c5\u6bd2\u8f09\u9ad4 | All-in-One Lentivector (Rat) \u8ca8\u865fK7055708"},"content":{"rendered":"

A1bg sgRNA CRISPR\/Cas9 All-in-One Lentivector (Rat) (Target 3) <\/strong>\u00a0| <\/strong>\u8ca8\u865f K7055708<\/strong><\/a><\/span><\/p>\n

NM_022258.2<\/a> | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro<\/a><\/p>\n

\u76f4\u63a5\u975e\u75c5\u6bd2\u8cea\u7c92\u8f49\u67d3\u7acb\u5373\u8868\u9054\uff0c\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\uff0c\u7528\u65bc\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u8868\u9054\u3002abm\u7684sgRNA\u50c5\u8a2d\u8a08\u7528\u65bcCas9 Nuclease\u3002 \u8acb\u67e5\u8a62\u5b9a\u5236sgRNA\u7528\u65bc\u57fa\u56e0\u7d44\u7de8\u8f2f\u4ee5\u5916\u7684\u5176\u4ed6\u76ee\u7684\uff08\u4f8b\u5982CRISPRa\uff09\u3002 \u5c0d\u65bc\u57fa\u56e0\u7d44\u8abf\u63a7\u4e2d\u7684CRISPR-Cas9\u7cfb\u7d71\u61c9\u7528\uff0c\u53c3\u95b1Cas9\u7a81\u8b8a\u86cb\u767d\uff08Nickase\uff0cNull\u7a81\u8b8a\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u96a8\u8457CRISPR Cas9\u7cfb\u7d71\u7684\u767c\u73fe\uff08Clustered Regular Interspaced Short Palindromic Repeats CRISPR-Associated Proteins 9\uff09\uff0c\u79d1\u5b78\u5bb6\u73fe\u5728\u80fd\u5920\u8f15\u9b06\u6709\u6548\u5730\u6572\u9664\u6216\u6572\u5165\u4efb\u4f55\u611f\u8208\u8da3\u7684\u57fa\u56e0\u3002 \u9451\u65bc\u5176\u6613\u65bc\u4f7f\u7528\uff0cRNA\u5f15\u5c0e\u7684CRISPR Cas9\u57fa\u56e0\u7d44\u7de8\u8f2f\u7cfb\u7d71\u63d0\u4f9b\u4e86\u4e00\u500b\u975e\u5e38\u591a\u529f\u80fd\u7684\u5e73\u53f0\uff0c\u4e26\u6709\u6f5b\u529b\u53d6\u4ee3\u5287\u70c8\u7684\u92c5\u6307\u548cTALEN\u65b9\u6cd5\u3002ABMGOOD\u63d0\u4f9b\u4e0d\u540c\u7d30\u80de\u4f86\u6e90\u4e4bCasp9\u7d30\u80de\u682a\uff1b\u5373\u7528\u578b\uff0c\u7d44\u6210\u578b\u8868\u9054\u6216\u53ef\u8a98\u5c0e\u7684Cas9\u7a69\u5b9a\u7d30\u80de\u7cfb\uff0c\u7d30\u80de\u682a\u7a2e\u985e\u8986\u84cb\u6d41\u884c\u7684\u816b\u7624\u6a21\u578b\u7d30\u80de\u7cfb\uff0c\u61c9\u7528\u65bcCRISPR\u4ecb\u5c0e\u7684\u57fa\u56e0\u6572\u9664\u4e26\u6572\u5165\u4efb\u4f55\u4eba\uff0c\u5c0f\u9f20\u6216\u5927\u9f20\u57fa\u56e0\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-SFFV-Cas9-2A-Puro<\/a><\/td>\n<\/tr>\n
Cas9 Promoter<\/strong><\/td>\nCas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. If necessary, the SFFV promoter can be replaced with EF1a promoter as a custom cloning service. Please inquire for pricing.<\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nTarget 3: Sequence available upon placing order<\/td>\n<\/tr>\n
Sequencing Primers<\/strong><\/td>\nU6 Forward Primer: 5\u2019–TACGTCCAAGGTCGGGCAGGAAGA–3\u2019<\/td>\n<\/tr>\n
Bacterial Selection<\/strong><\/td>\nAmpicillin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
SourceOrgan<\/strong><\/td>\nKidney<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nPolygonal<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nHEK293T cells stably expressing Cas9<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nPuromycin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/span> CRISPR\u6aa2\u6e2c\u5957\u7d44<\/a> | CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de\u682a<\/a> | CASPASE9 (D10A) NLS<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

A1bg sgRNA CRISPR\/Cas9 All-in-One Lentivector (Rat) (Ta […]<\/p>\n","protected":false},"author":3,"featured_media":10740,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[522],"tags":[3642],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10739"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10739"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10739\/revisions"}],"predecessor-version":[{"id":10748,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10739\/revisions\/10748"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10740"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10739"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10739"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10739"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}