{"id":10731,"date":"2017-06-14T14:26:47","date_gmt":"2017-06-14T06:26:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10731"},"modified":"2022-07-22T09:54:00","modified_gmt":"2022-07-22T01:54:00","slug":"mcf7-cas9-inducible%e7%b4%b0%e8%83%9e%e6%a0%aa-cas9-stably-integrated-mcf7-cell-lines%e8%b2%a8%e8%99%9ft3257","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=10731","title":{"rendered":"MCF7 Cas9 -inducible\u7d30\u80de\u682a | Cas9 stably integrated MCF7 cell lines\u8ca8\u865fT3357"},"content":{"rendered":"

MCF7 Cas9 stably integrated cell lines<\/strong> | <\/strong>\u8ca8\u865f<\/strong>T3357\u00a0<\/strong><\/span><\/p>\n

\"abm-casCellLine\"<\/p>\n

\u7d30\u80de\u682a\u540d\u7a31: MCF7 Cell Line (Cas9 expressing)-<\/strong>– \u00a0inducible Cas9 expression<\/p>\n

\u96a8\u8457CRISPR Cas9\u7cfb\u7d71\u7684\u767c\u73fe\uff08Clustered Regular Interspaced Short Palindromic Repeats CRISPR-Associated Proteins 9\uff09\uff0c\u79d1\u5b78\u5bb6\u73fe\u5728\u80fd\u5920\u8f15\u9b06\u6709\u6548\u5730\u6572\u9664\u6216\u6572\u5165\u4efb\u4f55\u611f\u8208\u8da3\u7684\u57fa\u56e0\u3002 \u9451\u65bc\u5176\u6613\u65bc\u4f7f\u7528\uff0cRNA\u5f15\u5c0e\u7684CRISPR Cas9\u57fa\u56e0\u7d44\u7de8\u8f2f\u7cfb\u7d71\u63d0\u4f9b\u4e86\u4e00\u500b\u975e\u5e38\u591a\u529f\u80fd\u7684\u5e73\u53f0\uff0c\u4e26\u6709\u6f5b\u529b\u53d6\u4ee3\u5287\u70c8\u7684\u92c5\u6307\u548cTALEN\u65b9\u6cd5\u3002ABMGOOD\u63d0\u4f9b\u4e0d\u540c\u7d30\u80de\u4f86\u6e90\u4e4bCasp9\u7d30\u80de\u682a\uff1b\u5373\u7528\u578b\uff0c\u7d44\u6210\u578b\u8868\u9054\u6216\u53ef\u8a98\u5c0e\u7684Cas9\u7a69\u5b9a\u7d30\u80de\u7cfb\uff0c\u7d30\u80de\u682a\u7a2e\u985e\u8986\u84cb\u6d41\u884c\u7684\u816b\u7624\u6a21\u578b\u7d30\u80de\u7cfb\uff0c\u61c9\u7528\u65bcCRISPR\u4ecb\u5c0e\u7684\u57fa\u56e0\u6572\u9664\u4e26\u6572\u5165\u4efb\u4f55\u4eba\uff0c\u5c0f\u9f20\u6216\u5927\u9f20\u57fa\u56e0\u3002ABMGOOD Casp9\u7d30\u80de\u682a\uff0c\u5177\u6709\u4ee5\u4e0b\u512a\u9ede:<\/p>\n

\u2714\u53ea\u9700\u4f7f\u7528sgRNA\u4f86\u9776\u5411\u611f\u8208\u8da3\u7684\u57fa\u56e0 \u3010\u66f4\u591a\u76ee\u6a19\u57fa\u56e0\u4e4bsgRNA lentiVetor\u00a0\u8acb\u6309\u6b64 <\/a><\/span>\u3011<\/p>\n

\u2714\u6700\u5c0f\u5316Cas9\u4ecb\u5c0e\u7684off-target effects<\/p>\n

\u2714 pre-made\u7a69\u5b9a\u7d30\u80de\u7cfb<\/p>\n

\u2714\u53ef\u7528\u65bc\u6d41\u884c\u7684\u7d30\u80de\u7cfb\u7814\u7a76\u6a21\u578b<\/p>\n

\u2714\u901a\u904eqPCR\u548c\/\u6216western\u5370\u8de1\u9810\u5148\u9a57\u8b49\u7684Cas9\u8868\u9054<\/p>\n

\u2714\u7528\u4f5c\u63a7\u5236\u7d30\u80de\u7cfb\u6bd4\u8f03gene editing efficiency\u57fa\u56e0\u7de8\u8f2f\u6548\u7387<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/td>\nII<\/td>\n<\/tr>\n
Organism<\/td>\nHuman<\/td>\n<\/tr>\n
SourceOrgan<\/td>\nMammary gland\/breast<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/td>\nEpithelial<\/td>\n<\/tr>\n
Description<\/td>\nMCF7 cells stably expressing cumate-inducible Cas9<\/td>\n<\/tr>\n
Markers<\/td>\nPuromycin & Neomycin<\/td>\n<\/tr>\n
Donor Age<\/td>\n69 year old<\/td>\n<\/tr>\n
Donor Gender<\/td>\nFemale<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/span> CRISPR\u6aa2\u6e2c\u5957\u7d44<\/a> | CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de\u682a<\/a> | CASPASE9 (D10A) NLS<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nCat No.<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Inducible Cas9 Expressing 293T Cell Line<\/a><\/td>\nT3351<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing 293 Cell Line<\/a><\/td>\nT3352<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing A549 Cell Line<\/a><\/td>\nT3353<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing HeLa Cell Line<\/a><\/td>\nT3354<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing HepG2 Cell Line<\/a><\/td>\nT3356<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing MCF7 Cell Line<\/a><\/td>\nT3357<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing K562 Cell Line<\/a><\/td>\nT3358<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing U-87 MG Cell Line<\/a><\/td>\nT3359<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n
Inducible Cas9 Expressing HT1080 Cell Line<\/a><\/td>\nT3360<\/td>\n1×106<\/sup>\u00a0cells\/1.0ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

MCF7 Cas9 stably integrated cell lines | \u8ca8\u865fT3357\u00a0 \u7d30\u80de\u682a\u540d\u7a31 […]<\/p>\n","protected":false},"author":3,"featured_media":10674,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[522],"tags":[3644],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10731"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10731"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10731\/revisions"}],"predecessor-version":[{"id":10734,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10731\/revisions\/10734"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10674"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10731"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10731"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10731"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}