{"id":10414,"date":"2017-05-23T13:00:15","date_gmt":"2017-05-23T05:00:15","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10414"},"modified":"2022-07-22T10:24:47","modified_gmt":"2022-07-22T02:24:47","slug":"%e8%97%a5%e7%89%a9%e7%af%a9%e9%81%b8-picolorlock%e7%a3%b7%e9%85%b8%e6%a0%b9%e9%85%b5%e7%b4%a0%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90malachite-green%e7%a3%b7%e9%85%b8%e6%a0%b9%e5%91%88%e8%89%b2","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=10414","title":{"rendered":"\u85e5\u7269\u7be9\u9078 | PiColorLock\u78f7\u9178\u6839\u9175\u7d20\u6d3b\u6027\u5206\u6790\/Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5 \u8ca8\u865f303-0125"},"content":{"rendered":"

\u9175\u7d20\u6d3b\u6027\u5206\u6790\/ \u6291\u5236\u7269\u7be9\u9078\/ \u85e5\u7269\u7be9\u9078<\/span><\/strong><\/p>\n

\u9ad8\u9748\u654f\u5ea6Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5 |\u8ca8\u865f303-0125\uff08\u9748\u654f\u5ea64\uff0d40uM\uff09<\/a><\/span><\/p>\n

PiColorLock Gold Phosphate Detection System<\/span><\/strong><\/span><\/p>\n

\"\"PiColorLock\u2122\u78f7\u9178\u6839\u6aa2\u6e2c\u8a66\u5291\uff0c\u5176\u539f\u7406\u70ba\u6aa2\u6e2c\u7121\u6a5f\u78f7\u9178\u6839\uff08Pi\uff09\u4e4b\u542b\u91cf\uff1b\u4f7f\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\u3002\u53ef\u7528\u65bc\u6e2c\u91cf\u6703\u7522\u751fPi\u7684\u4efb\u4f55\u9176\uff0c\u5305\u62ecATPases\u3001GTPases, phosphatases\u3001Kinase\u3001Nucleotidases\u7b49\u9175\u7d20\u6d3b\u6027\u4e4b\u5206\u6790\u3002PiColorLock\u2122Gold\u57fa\u65bcmalachite green-based assay\u5b54\u96c0\u77f3\u7da0\u7684\u6e2c\u5b9a\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u00a0free phosphate\u7121\u6a5f\u78f7\u9178\u9e7d\u6c34\u6eb6\u6db2\u3002 \u8a72\u6e2c\u5b9a\u4f9d\u8cf4\u65bc\u5b54\u96c0\u77f3\u7da0\u4e4b\u9593\u5f62\u6210\u8907\u5408\u7269\u926c\u9178\u9e7d\u548cfree orthophosphate\u6e38\u96e2\u6b63\u78f7\u9178\u9e7d\uff0c\u6eb6\u6db2\u984f\u8272\u5f9eorange\u6a59\u8272\u8b8a\u70ba\u7da0\u8272\u00a0green\u3002\u984f\u8272\u7684\u5f62\u6210\u53ef\u4ee5\u65b9\u4fbf\u5730\u5728\u5206\u5149\u5149\u5ea6\u8a08\uff08620-650nm\uff09\u4e0a\u6216\u5728\u5206\u5149\u5149\u5ea6\u8a08\u4e0a\u6e2c\u91cf\uff0c\u8b80\u503c\u8207\u6e38\u96e2\u6b63\u78f7\u9178\u9e7d\u6fc3\u5ea6\u76f4\u63a5\u76f8\u95dc\u3002\u00a0PiColorLock\u2122\u7684\u7368\u7279\u914d\u65b9\u63d0\u9ad8\u4e86\u6e2c\u5b9a\u7dda\u6027\uff0c\u52d5\u614b\u7bc4\u570d\u548c\u984f\u8272\u7a69\u5b9a\u6027\uff1b\u4e5f\u53ef\u4ee5\u7528PiColorLock\u8207\u4e0d\u7a69\u5b9a\u7684\u5e95\u7269\uff08\u4f8b\u5982ATP\uff0cGTP\uff09\u4e00\u8d77\u4f7f\u7528\uff0c\u6240\u8ff0\u5e95\u7269\u8207\u5176\u5b83\u9178\u6027\u67d3\u6599\u57fa\u6aa2\u6e2c\u8a66\u5291\u7522\u751f\u9ad8\u7684\u975e\u9176\u80cc\u666f\u4fe1\u865f\u3002\u6b64\u7522\u54c1\u70ba\u4e0d\u5177\u653e\u5c04\u6027\u61c9\u7528Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5\uff0c\u7a69\u5b9a\u7684\u7522\u751f\u7da0\u8272dye-phosphate\u8907\u5408\u7269\uff0cPi-dye\u7a69\u5b9a\u6027\u8907\u5408\u7269\u65bc\u9577\u6642\u9593\u4e4b\u6e2c\u91cf\uff0c\u4e0d\u6613\u7522\u751f\u53cd\u61c9\u7269\u6c89\u6fb1(30\u5206\u5f8c)\u554f\u984c\u800c\u5f71\u97ff\u6e2c\u91cf\u8b80\u503c\u3002\u5075\u6e2c\u7bc4\u570d\u5ee3\uff0c\u5728\u86cb\u767d\u8cea\u6fc3\u5ea6\u9ad8\u4e0b\u4e0d\u6703\u6291\u5236\u984f\u8272\u4e4b\u5f62\u6210\uff1b\u53ef\u6e1b\u5c11\u6a23\u54c1\u7a00\u91cb\u7684\u5fc5\u8981\u3002\u53e6\u63d0\u4f9bStabilizer\u5728\u9178\u53ef\u6eb6\u6027\u53d7\u8cea\u4e0b\uff0c\u907f\u514d\u767c\u751f\u53d7\u8cea\u975e\u9175\u7d20\u6c34\u89e3\u5e72\u64fe\u9020\u6210\u7684\u9ad8\u80cc\u666f\u96dc\u8a0a\u3002\"picolorlock_web\"<\/p>\n

PiColorLock\u2122 \u53ef\u7528\u65bc\u6e2c\u91cf\u4ee5\u4e0b\u6e38\u96e2\u6b63\u78f7\u9178\u9e7d\u7684\u91cb\u653e<\/strong><\/p>\n

1. Phosphatase Assays\u78f7\u9178\u9176\u6e2c\u5b9a\uff1a\u5f9epeptide\uff0cprotein\u86cb\u767d\u8cea\u6216\u00a0small molecule substrate\u5c0f\u5206\u5b50\u5e95\u7269\u4e2d\u91cb\u653e\u78f7\u9178\u9e7d
\n2. Lipase Assays\u8102\u80aa\u9176\u6e2c\u5b9a\uff1a\u5f9e\u78f7\u8102\u4e2d\u91cb\u653ephospholipids\u78f7\u9178\u9e7d
\n3. Nucleoside Triphosphatase Assays\u6838\u82f7\u4e09\u78f7\u9178\u9176\u6e2c\u5b9a\uff1a\u5f9enucleoside triphosphates\u6838\u82f7\u4e09\u78f7\u9178\u4e2d\u91cb\u653e\u78f7\u9178\u9e7d\uff08ATP\uff0c
\nGTP\uff0cTTP\uff0cCTP\uff09
\n4. Quantitation\u00a0\u5b9a\u91cfphospholipids\u78f7\u8102\uff0cproteins\u86cb\u767d\u8cea\u548cDNA\u4e2d\u7684phosphate\u78f7\u9178\u9e7d
\n5. Drug Discovery\u85e5\u7269\u767c\u73fe\uff1a\u78f7\u9178\u9176\u6291\u5236\u5291\u7684\u9ad8\u901a\u91cf\u7be9\u9078\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u578b\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
303-0125
\n303-0500<\/td>\n		PiColorLock Gold Phosphate Detection System<\/a>\uff08\u9748\u654f\u5ea64\uff0d40uM\uff09<\/td>\n\u6700\u591a1560 assays
\n\u6700\u591a6250 assays<\/td>\n<\/tr>\n
\u95dc\u806f\u5546\u54c1<\/strong><\/span><\/td>\n<\/tr>\n
601-9999<\/td>\n10mM Lyophilized ATP<\/a><\/td>\n0.5ml<\/td>\n<\/tr>\n
602-0120
\n602-0121
\n602-0122<\/td>\n		High Throughput Colorimetric GTPase Assays<\/a><\/td>\n196 assays
\n480 assays
\n5 \u00d7 480 assays<\/td>\n<\/tr>\n
501-0015<\/td>\nPiBind resin<\/a>\uff0d\u5feb\u901f\u79fb\u9664\u6a23\u54c1\u4e2d\u6c61\u67d3\u7684\u78f7\u9178\u6839\uff08Pi\uff09<\/td>\n5g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u9175\u7d20\u6d3b\u6027\u5206\u6790\/ \u6291\u5236\u7269\u7be9\u9078\/ \u85e5\u7269\u7be9\u9078 \u9ad8\u9748\u654f\u5ea6Malachite green\u78f7\u9178\u6839\u5448\u8272\u6cd5 |\u8ca8\u865f303-01 […]<\/p>\n","protected":false},"author":3,"featured_media":10418,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[457],"tags":[3684],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10414"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10414"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10414\/revisions"}],"predecessor-version":[{"id":18958,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10414\/revisions\/18958"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10418"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10414"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10414"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10414"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}