{"id":10304,"date":"2017-05-18T16:02:15","date_gmt":"2017-05-18T08:02:15","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10304"},"modified":"2022-07-22T10:26:32","modified_gmt":"2022-07-22T02:26:32","slug":"hek293s-tetr-tp%e7%b4%b0%e8%83%9e%e6%a0%aa-hek293s-tetr-stably-expressing-tp-cell-line-%e8%b2%a8%e8%99%9ft3021","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=10304","title":{"rendered":"HEK293S-TetR TP\u7d30\u80de\u682a | HEK293S-TetR Stably Expressing TP Cell Line (\u8ca8\u865fT3021)"},"content":{"rendered":"<p><span style=\"font-size: 14pt; color: #ff6600;\"><strong>\u85e5\u7269\u6a19\u9776<\/strong><strong> | <\/strong><strong>Drug Discovery-<\/strong><strong>\u7d30\u80de\u5e73\u53f0\u7cfb\u5217\u5546\u54c1<\/strong><\/span><\/p>\n<p>Prostanoid Receptors Drug Discovery Stable Cell Lines<\/p>\n<p><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10304\">HEK293S-TetR Stably Expressing TP Cell Line\u00a0(\u8ca8\u865fT3021)<\/a><\/span><\/p>\n<p><strong>\u7d30\u80de\u6c34\u5e73\u7684\u85e5\u7269\u7be9\u9078<\/strong>\uff0c\u662f\u63a5\u8fd1\u751f\u7406\u689d\u4ef6\u7684\u4e00\u7a2e\u85e5\u7269\u7be9\u9078\u6a21\u5f0f\uff1b\u6392\u9664\u751f\u5316\u6c34\u5e73\u4e4b\u85e5\u7269\u7be9\u9078\u6a21\u578b\uff0c\u4e4b\u6613\u53d7\u9776\u9176\u7684\u4f5c\u7528\u7a0b\u5ea6\uff0c\u5438\u6536\u3001\u5206\u5e03\u3001\u4ee3\u8b1d\u3001\u6392\u6cc4\u7b49\u53c3\u6578\u4e0d\u78ba\u5b9a\u56e0\u7d20\u5f71\u97ff\uff0c\u4f7f\u8aa4\u7be9\u7387\u66f4\u9ad8\u3002\u7d30\u80de\u6c34\u5e73\u4e4b\u85e5\u7269\u7be9\u9078\uff0c\u66f4\u63a5\u8fd1\u751f\u7406\u689d\u4ef6\uff0c\u8b93\u7be9\u9078\u7684\u6e96\u78ba\u7387\u66f4\u9ad8\u3002Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u7d30\u80de\u6c34\u5e73\u4e4b\u85e5\u7269\u6a21\u578b\uff0c\u52a0\u901f\u65b0\u85e5\u767c\u73fe\u548c\u751f\u7269\u91ab\u5b78\u7814\u7a76\u3002\u7d93\u904e\u9a57\u8b49\u751f\u6210\u4e86\u4e00\u500b\u7368\u7279\u7684G\u86cb\u767d\u5076\u806f\u53d7\u9ad4\uff08GPCR\uff09\u5eab\u3001\u6fc0\u9176\u3001\u6838\u6fc0\u7d20\u53d7\u9ad4\uff08NHR\uff09\u548c\u96e2\u5b50\u901a\u9053\u4e4b\u7a69\u5b9a\u7d30\u80de\u7cfb\u5eab\uff0c\u70ba\u6240\u6709\u7814\u7a76\u9805\u76ee\u63d0\u4f9b\u4e86\u5f37\u5927\u7684\u6aa2\u6e2c\u5e73\u53f0\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/StableCellLines\/pics\/T3162%20Figure%202.jpg\" alt=\"\" width=\"352\" height=\"496\" \/><\/p>\n<p><strong>HEK293S-TetR \u7a69\u5b9a\u7684TP\u7d30\u80de<\/strong><strong>\u682a<\/strong><strong>\u4ecb\u7d39<\/strong><strong>:<\/strong> HEK293S-TetR\u7a69\u5b9a\u8868\u9054TP\u7d30\u80de\u682a\u88ab\u5efa\u7acb\u70ba\u5177\u6709\u8868\u9054thromboxane A2 receptor \u8840\u6813\u7d20A2\u53d7\u9ad4\uff08TP\uff09\u7684\u56db\u74b0\u7d20\u8a98\u5c0e\u578bHEK293S\u7a69\u5b9a\u7d30\u80de\u7cfb\u3002 TP\u662f\u53c3\u8207\u8840\u7ba1\u6536\u7e2e\u548c\u8840\u6813\u5f62\u6210\u7684G\u86cb\u767d\u5076\u806f\u53d7\u9ad4\uff08GPCR\uff09\u3002 \u53ef\u61c9\u7528\u65bc\u767c\u73feTP\u751f\u7269\u5b78\u548c\u53d7\u9ad4\u7684\u4f5c\u7528\u548c\u529f\u80fd\u4e0a\u6709\u50f9\u503c\u7684\u5de5\u5177\u3002abm\u5177\u6709\u53ef\u7528\u7684TP\u53d7\u9ad4\u7684A160T\u7a81\u8b8a\u9ad4\uff08\u8ca8\u865fT3164\uff09\u3002<span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1371\/journal.pone.0076481\">\u5f15\u7528\u6587\u737b\u6b64\u8acb\u6309\u6b64<\/a><\/span><\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>BioSafety Level<\/td>\n<td>II<\/td>\n<\/tr>\n<tr>\n<td>Organism<\/td>\n<td>Homo sapiens<\/td>\n<\/tr>\n<tr>\n<td>SourceOrgan<\/td>\n<td>Kidney<\/td>\n<\/tr>\n<tr>\n<td>Growth Properties<\/td>\n<td>Adherent<\/td>\n<\/tr>\n<tr>\n<td>Morphology<\/td>\n<td>Polygonal<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0<img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/StableCellLines\/pics\/T3162%20Figure%203.jpg\" alt=\"\" width=\"287\" height=\"478\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/StableCellLines\/pics\/T3162%20Figure%204.jpg\" alt=\"\" width=\"399\" height=\"479\" \/><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u85e5\u7269\u6a19\u9776 | Drug Discovery-\u7d30\u80de\u5e73\u53f0\u7cfb\u5217\u5546\u54c1 Prostanoid Receptors Dru [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[457],"tags":[3696],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10304"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10304"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10304\/revisions"}],"predecessor-version":[{"id":10309,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10304\/revisions\/10309"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10304"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10304"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}