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Mouse Anti-Human IgG4 pFc’-HRP抗體 | 貨號9190-05 規格1.0 mL

Primary Antibodies
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https://www.southernbiotech.com
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Mouse Anti-Human IgG4 pFc'-HRP抗體操作手冊

特點

Mouse Anti-Human IgG4 pFc’-HRP抗體 | 貨號9190-05 規格1.0 mL

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體,目前除了一般常見的多株與單株二級抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。

ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG4 pFc’-HRP (SB Cat. No. 9190-05).

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白(immunoglobulin , Ig)分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用,並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用,例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記,這使得能夠通過比色,化學發光和螢光技術進行檢測。例如,酶辣根過氧化物酶(enzymes horseradish peroxidase , HRP)和鹼性磷酸酶(alkaline phosphatase , AP)通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素(fluorescein , FITC),藻紅蛋白(phycoerythrin , PE)和花青(cyanine , CY)的螢光染料在螢光的應用中是必不可少的,包括流式細胞術,免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大,可增加的靈敏度。

產品介紹:  Mouse Anti-Human IgG4 pFc’-HRP抗體,具HRP標誌。針對Human IgG4 pFc’具特異性。免疫來源為Human IgG4 myeloma protein。

Isotype為Mouse (BALB/c) IgG3κ。Isotype Control Mouse IgG3-HRP。 緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

 

Clone HP6023
Isotype Mouse (BALB/c) IgG3κ
Isotype Control Mouse IgG3-HRP
Immunogen Human IgG4 myeloma protein
Specificity Human IgG4 pFc’
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications

Applications for relevant formats of this clone include –

ELISA – Quality tested 2-9
FLISA – Quality tested
Flow Cytometry – Reported in literature 12-14
Immunohistochemistry-Frozen Sections – Reported in literature 10
Western Blot – Reported in literature 11
Immunoprecipitation – Reported in literature 1
Surface Plasmon Resonance – Reported in literature 15

Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796684

References

  1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen, IP)
  2. Tomana M, Novak J, Julian BA, Matousovic K, Konecny K, Mestecky J. Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies. J Clin Invest. 1999;104:73-81. (ELISA)
  3. Whary MT, Sundina N, Bravo LE, Correa P, Quinones F, Caro F, et al. Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev. 2005;14:1464-9. (ELISA)
  4. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
  5. Itoh M, Weerasooriya MV, Yahathugoda TC, Takagi H, Samarawickrema WA, Nagaoka F, et al. Effects of 5 rounds of mass drug administration with diethylcarbamazine and albendazole on filaria-specific IgG4 titers in urine: 6-year follow-up study in Sri Lanka. Parasitol Int. 2011;60:393-7. (ELISA)
  6. Olaru F, Wang X, Luo W, Ge L, Miner JH, Kleinau S, et al. Proteolysis breaks tolerance toward intact α345(IV) collagen, eliciting novel anti-glomerular basement membrane autoantibodies specific for α345NC1 hexamers. J Immunol. 2013;190:1424-32. (ELISA)
  7. Ferreira AR, Singh B, Cabrera-Mora M, Magri De Souza AC, Queiroz Marques MT, Porto LC, et al. Evaluation of naturally acquired IgG antibodies to a chimeric and non-chimeric recombinant species of Plasmodium vivax reticulocyte binding protein-1: lack of association with HLA-DRB1*/DQB1* in malaria exposed individuals from the Brazilian Amazon. PLoS One. 2014;9(8):e105828. (ELISA)
  8. Haag S, Schneider N, Mason DE, Tuncel J, Andersson IE, Peters EC, et al. Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. Arthritis Rheumatol. 2014;66:1440-9. (ELISA)
  9. Lin J, Markowitz GS, Valeri AM, Kambham N, Sherman WH, Appel GB, et al. Renal monoclonal immunoglobulin deposition disease: the disease spectrum. J Am Soc Nephrol. 2001;12:1482-92. (IHC-FS)
  10. de Vos AF, Melief M, van Riel D, Boon L, van Eijk M, de Boer M, et al. Antagonist anti-human CD40 antibody inhibits germinal center formation in cynomolgus monkeys. Eur J Immunol. 2004;34:3446-55. (IHC-FS)
  11. Fischer PU, Curtis KC, Folk SM, Wilkins PP, Marcos LA, Weil GJ. Serological diagnosis of North American Paragonimiasis by Western blot using Paragonimus kellicotti adult worm antigen. Am J Trop Med Hyg. 2013;88:1035-40. (WB)
  12. Liles WC, Dale DC, Price TH, Gaviria JM, Turner T, Saoud J, et al. Inhibition of in vivo neutrophil transmigration by a novel humanized anti-CD11/CD18 monoclonal antibody. Cytokines Cell Mol Ther. 2000;6:121-6. (FC)
  13. Verbon A, Dekkers PE, ten Hove T, Hack CE, Pribble JP, Turner T, et al. IC14, an anti-CD14 antibody, inhibits endotoxin-mediated symptoms and inflammatory responses in humans. J Immunol. 2001;166:3599-605. (FC)
  14. Toyoda C, Suzuki Y, Tsuneyama H, Onodera T, Masuno A, Yabe R, et al. Production of human monoclonal anti-Jk3, recognising an epitope including the Jka/Jkb polymorphic site of the Kidd glycoprotein. Transfus Med. 2014;24:286-91. (FC)
  15. Lewis KB, Hughes RJ, Epstein MS, Josephson NC, Kempton CL, Kessler CM, et al. Phenotypes of allo- and autoimmune antibody responses to FVIII characterized by surface plasmon resonance. PLoS One. 2013;8(5):e61120. (Surface Plasmon Resonance)
Gene ID 3503 (Human)
Gene Symbol IGHG4 (Human)
Uniprot ID P01861 (Human)
Uniprot Name IGHG4_HUMAN

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

 

 

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