Ki 67單株抗體 | 貨號ABM40064

Ki 67 Monoclonal Antibody

產品描述： MKI67編碼與細胞增殖(cellular proliferation)有關並且可能是細胞增殖(cellular proliferation)所必需的核蛋白（增殖Ki-67的標記）。已經描述了剪接的轉錄物變體。 一個相關的假基因(pseudogene)存在於X染色體上。

Fig.1. IHC-P staining of mouse kidney tissue, diluted at 1:200.

Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of mouse testis tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.4. Immunofluorescence analysis of rat brain tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

• Product name

Ki 67 Monoclonal Antibody

• Immunogen

Synthetic Peptide

• Host

Mouse

• Reactivity

Human, Mouse, Rat

• Applications

IF, IHC-P

• Application notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:200).

• Clonality

Monoclonal

• Isotype

Mouse IgG1

• Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

• Background

MKI67 encodes a nuclear protein (marker of proliferation Ki-67) that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

• Gene ID

4288

• Alternative names

MKI67; Antigen KI-67

• Others

The antibody detects endogenous Ki 67 proteins.

• Accession

P46013

 

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