♦♦♦ Bioassay Technology Laboratory (BT Lab) 台灣代理商-太鼎生物科技
Shanghai Crystal Day Biotech成立於2009年,前身為Shanghai Korain Biotech,與大學實驗室合作,旨在為實驗室研究人員提供優質、實惠的ELISA工具。 經過五年的研究和改進,Shanghai Korain Biotech成為ELISA試劑盒的專有平台,並創立了“bt-laboratory(簡稱BT LAB)
Rotavirus antigen ELISA試劑盒 | Human Rotavirus antigen, RV Ag ELISA Kit貨號ED0076Hu
產品描述:Rotavirus antigen ELISA試劑盒, 用於定性(Qualitative)樣品中的Rotavirus antigen(RV Ag)。測量物種: Human。該試劑盒基於定性反相酶免疫分析技術(reverse phase enzyme immuno assay technique)。 微量滴定板已預先塗有目標抗體。 將陽性/陰性對照或樣品加入孔中並孵育。 樣品中的抗原與板上的抗體結合。 未結合的抗原在洗滌步驟中被洗掉。 然後加入辣根過氧化物酶 (Horseradish Peroxidase , HRP) 偶聯的檢測抗體並孵育。 未結合的 HRP 在洗滌步驟中被洗掉。 然後加入 TMB 底物並顯色。 通過加入酸性終止溶液終止反應,顏色變為黃色,可在 450 nm 處測量。 然後可以將未知樣品的 OD 與陽性和陰性對照的 OD 進行比較,以確定 RV Ag 的存在。可應用於以下樣品來源: Serum, Plasma, Cell culture supernatant
Product Summary
Size: 96 Test
Assay type: Qualitative
Sample type: Serum, Plasma, Cell culture supernatant
Reactive with: Human
Shanghai Crystal Day Biotech成立於2009年,前身為Shanghai Korain Biotech,與大學實驗室合作,旨在為實驗室研究人員提供優質、實惠的ELISA工具。 經過五年的研究和改進,Shanghai Korain Biotech成為ELISA試劑盒的專有平台,並創立了“bt-laboratory(簡稱BT LAB)”品牌,擁有6, 000+ ELISA試劑盒的豐富產品線 包括夾心ELISA(酶聯免疫吸附測定)試劑盒、Competitive EIA(酶免疫測定)試劑盒。 2018 年,BT LAB 推出了擁有 10,000+ 多種抗體的抗體系列及10,000+ ELISA套組。2019年10月,我們的實驗室和倉庫在浙江嘉興建成。 現在 Korain 在 5 大洲的 35 個國家/地區發展壯大,引用次數超過 6,000+。
酶聯免疫吸附試驗 (enzyme-linked immunosorbent assay , ELISA) 是一種常用的分析生化分析方法(biochemistry assay)。 這測定使用固相類型的酶免疫測定(enzyme immunoassay)檢測液體樣品中蛋白質的存在使用針對蛋白質的抗體測量。 在最簡單的 ELISA 形式中,抗原從樣品(如血清、血漿、細胞培養上清液、細胞裂解液、唾液、組織裂解液和尿液)被測試的物體附著在一個表面上。 然後,一個匹配的抗體被塗在表面上,所以它可以結合抗原。 這種抗體與酶相連然後去除任何未結合的抗體。 在最後一步,含有酶的物質添加底物。 如果有綁定後續反應產生可檢測的信號,最常見的是顏色變化。
High sensitivity and specificity ELISA kit Sensitivity of the assay is the smallest detectable amount of the target protein in question, it depends mainly on the affinity of the solid phase antibody according to the law of mass action. Specificity helps us recognize if the antibody detect the target protein specifically without crossreacting with non-pecific proteins. Monoclonal antibodies have very high affinity for the antigen, which also have higher specificity than polyclonal antibodies. A monoclonal antibody is used as the detecting antibody in BT LAB sandwich ELISA to provide improved specificity. • Extensive analyte selection • Strict QC control • Vadidated sample testing
高靈敏度(sensitivity)和特異性(specificity )ELISA試劑盒
測定的靈敏度(sensitivity)是所討論的目標蛋白質的最小可檢測量,它根據質量作用定律,主要取決於固相抗體的親和力。特異性幫助我們識別抗體是否特異性檢測目標蛋白質,而不會與非特異性蛋白質發生交叉反應。 單克隆抗體對抗原有很高的親和力,其特異性也高於多克隆抗體。 單克隆抗體用作BT LAB 夾心 ELISA 中的檢測抗體可提供更高的特異性。
- 廣泛的分析物選擇
- 嚴格的質量控制
- 驗證樣本測試
精度和再現性Precision & Reproducibility
精密度(Precision)是作為平均值的變異係數 (variation , CV) 來衡量的。
CV 應根據計算出的濃度而不是原始光學值來計算密度。
- 小於15 的分析間% CV 通常是可以接受的。
- 檢測內 % CV 應小於 10。
- 報告反映結果範圍的濃度的 CV 很重要標本中發現。
BT Lab ELISA Types There are several ELISA configurations, with the most common formats being sandwich, competitive and signaling assays. Concentration-dependent detection of the target analyte is accompanied by either an increase or a decrease in signal, which is related to the format that is chosen.
E0000Mk | Monkey | Monkey Hyaluronic acid,HA ELISA Kit | 96 wells/48 wells |
E0001Mk | Monkey | Monkey Apolipoprotein C3,Apo C3 ELISA Kit | 96 wells/48 wells |
E0002MK | Monkey | Monkey Prostaglandin E2,PG-E2 ELISA Kit | 96 wells/48 wells |
E0004MK | Monkey | Monkey Hemoglobin subunit beta,HBB ELISA Kit | 96 wells/48 wells |
E0005MK | Monkey | Monkey transforming growth factor α,TGF-α ELISA Kit | 96 wells/48 wells |
E0007MK | Monkey | Monkey Lymphotoxin-beta,LTB ELISA Kit | 96 wells/48 wells |
E0009MK | Monkey | Monkey adiponectin,ADP ELISA Kit | 96 wells/48 wells |
E0011MK | Monkey | Monkey Glutathione reductase,GR ELISA Kit | 96 wells/48 wells |
E0012MK | Monkey | Monkey secretory immunoglobulin A,SIgA ELISA Kit | 96 wells/48 wells |
E0013MK | Monkey | Monkey Microalbunminuria,MAU/ALB ELISA Kit | 96 wells/48 wells |
E0014MK | Monkey | Monkey TEK tyrosine kinase,Endothelial,Tie2 ELISA Kit | 96 wells/48 wells |
E0015MK | Monkey | Monkey Interleukin 17,IL-17 ELISA Kit | 96 wells/48 wells |
E0016MK | Monkey | Monkey Prolactin,PRL ELISA Kit | 96 wells/48 wells |
E0017MK | Monkey | Monkey mast cell tryptase,MCT ELISA Kit | 96 wells/48 wells |
E0018MK | Monkey | Monkey Dihydrotestosterone,DHT ELISA Kit | 96 wells/48 wells |
E0019MK | Monkey | Monkey Insulin,INS ELISA Kit | 96 wells/48 wells |
E0020MK | Monkey | Monkey Histamine,HIS ELISA Kit | 96 wells/48 wells |
E0021MK | Monkey | Monkey Complement fragment 5a,C5a ELISA Kit | 96 wells/48 wells |
E0022MK | Monkey | Monkey Complement fragment 3a,C3a ELISA Kit | 96 wells/48 wells |
E0024MK | Monkey | Monkey S100 calcium binding protein A4,S100A4 ELISA Kit | 96 wells/48 wells |
E0025MK | Monkey | Monkey matrix metalloproteinase,MMP-7 ELISA Kit | 96 wells/48 wells |
E0026MK | Monkey | Monkey neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit | 96 wells/48 wells |
E0027MK | Monkey | Monkey tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit | 96 wells/48 wells |
E0029MK | Monkey | Monkey islet cell antibody,ICA ELISA Kit | 96 wells/48 wells |
E0030MK | Monkey | Monkey C-Peptide,C-P ELISA Kit | 96 wells/48 wells |
E0031MK | Monkey | Monkey Interleukin 1α,IL-1α ELISA Kit | 96 wells/48 wells |
E0032MK | Monkey | Monkey Interleukin 4,IL-4 ELISA Kit | 96 wells/48 wells |
E0033MK | Monkey | Monkey Interleukin 5,IL-5 ELISA Kit | 96 wells/48 wells |
E0034MK | Monkey | Monkey Interleukin 6,IL-6 ELISA Kit | 96 wells/48 wells |
E0035MK | Monkey | Monkey Interleukin 10,IL-10 ELISA Kit | 96 wells/48 wells |
E0036MK | Monkey | Monkey Transforming Growth factor β1,TGF-β1 ELISA Kit | 96 wells/48 wells |
E0037MK | Monkey | Monkey Transforming Growth factor β2,TGF-β2 ELISA Kit | 96 wells/48 wells |
E0038MK | Monkey | Monkey Interleukin 1β,IL-1β ELISA Kit | 96 wells/48 wells |
E0039MK | Monkey | Monkey C-reactive protein,CRP/PTX1 ELISA Kit | 96 wells/48 wells |
E0040MK | Monkey | Monkey Serum Amyloid A2,SAA2 ELISA Kit | 96 wells/48 wells |
E0041MK | Monkey | Monkey Regenerating Islet Derived Protein 3 Alpha,REG3a ELISA Kit | 96 wells/48 wells |
E0042MK | Monkey | Monkey Hemoglobin,HB ELISA Kit | 96 wells/48 wells |
E0043MK | Monkey | Monkey cytochrome P450,CYP450 ELISA Kit | 96 wells/48 wells |
E0044MK | Monkey | Monkey Cytochrome P450 3A4,CYP3A4 ELISA Kit | 96 wells/48 wells |
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E0047MK | Monkey | Monkey Myxovirus Resistance 1,MX1 ELISA Kit | 96 wells/48 wells |
E0049MK | Monkey | Monkey Adrenocorticotropic Hormone,ACTH ELISA Kit | 96 wells/48 wells |
E0050MK | Monkey | Monkey Serotonin,ST ELISA Kit | 96 wells/48 wells |
E0051MK | Monkey | Monkey Interferon γ ,IFN-γ ELISA Kit | 96 wells/48 wells |
E0052MK | Monkey | Monkey Luteinizing Hormone,LH ELISA Kit | 96 wells/48 wells |
E0053MK | Monkey | Monkey Fructosamine,FTA ELISA Kit | 96 wells/48 wells |
E0087MK | Monkey | Monkey Malondialdehyde ELISA KIT | 96 wells/48 wells |
E0088MK | Monkey | Monkey Tumor necrosis factor α,TNF-α ELISA Kit | 96 wells/48 wells |
E0090MK | Monkey | Monkey Substance P ELISA kit | 96 wells/48 wells |
E0091MK | Monkey | Monkey Hepatitis A virus cellular receptor 1 homolog,HAVCR1/KIM-1 ELISA KIT | 96 wells/48 wells |
E0092MK | Monkey | Monkey D-Dimer,D2D ELISA Kit | 96 wells/48 wells |
E0093MK | Monkey | Monkey amyloid beta peptide 1-42,Aβ1-42 ELISA Kit | 96 wells/48 wells |
E0094MK | Monkey | Monkey Beta-defensin 1,DEFB1 ELISA kit | 96 wells/48 wells |
E0095MK | Monkey | Monkey Beta-defensin 123,DEFB123 ELISA kit | 96 wells/48 wells |
E0096MK | Monkey | Monkey Beta-microseminoprotein,MSMB ELISA kit | 96 wells/48 wells |
E0097MK | Monkey | Monkey brain natriuretic peptide,BNP ELISA Kit | 96 wells/48 wells |
E0098MK | Monkey | Monkey Brain-derived neurotrophic factor,BDNF ELISA kit | 96 wells/48 wells |
E0099MK | Monkey | Monkey Cathelicidin antimicrobial peptide,CAMP ELISA kit | 96 wells/48 wells |
E0100MK | Monkey | Monkey C-C motif chemokine 17,CCL17 ELISA kit | 96 wells/48 wells |
E0101MK | Monkey | Monkey Chymotrypsin-C,CTRC ELISA kit | 96 wells/48 wells |
E0102MK | Monkey | Monkey circulating immune complex,CIC ELISA Kit | 96 wells/48 wells |
E0103MK | Monkey | Monkey Complement 3,C3 ELISA Kit | 96 wells/48 wells |
E0104MK | Monkey | Monkey Complement 4,C4 ELISA kit | 96 wells/48 wells |
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E0107MK | Monkey | Monkey Cysteine-rich secretory protein 1,CRISP1 ELISA kit | 96 wells/48 wells |
E0108MK | Monkey | Monkey Cytochrome P450 1A1,CYP1A1 ELISA kit | 96 wells/48 wells |
E0109MK | Monkey | Monkey Endothelin 1,ET-1 ELISA Kit | 96 wells/48 wells |
E0110MK | Monkey | Monkey Erythropoietin,EPO ELISA kit | 96 wells/48 wells |
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E0112MK | Monkey | Monkey Glutathione S-transferase P,GSTP1 ELISA kit | 96 wells/48 wells |
E0113MK | Monkey | Monkey Growth hormone receptor,GHR ELISA kit | 96 wells/48 wells |
E0115MK | Monkey | Monkey IgG receptor FcRn large subunit p51,FCGRT ELISA kit | 96 wells/48 wells |
E0116MK | Monkey | Monkey immunoglobulin A,IgA ELISA kit | 96 wells/48 wells |
E0117MK | Monkey | Monkey Immunoglobulin M,IgM ELISA kit | 96 wells/48 wells |
E0118MK | Monkey | Monkey Insulin-like Growth Factor 1,IGF-1 ELISA KIT | 96 wells/48 wells |
E0119MK | Monkey | Monkey Interferon α,IFN-α ELISA Kit | 96 wells/48 wells |
E0120MK | Monkey | Monkey Interferon β,IFN-β ELISA Kit | 96 wells/48 wells |
E0121MK | Monkey | Monkey interleukin 16,lymphocyte chemoattractant factor,IL16 ELISA kit | 96 wells/48 wells |
E0122MK | Monkey | Monkey Interleukin-2 receptor,IL-2R ELISA kit | 96 wells/48 wells |
E0123MK | Monkey | Monkey Interleukin-8,IL-8 ELISA Kit | 96 wells/48 wells |
E0124MK | Monkey | Monkey macrophage colony-stimulating factor,M-CSF ELISA kit | 96 wells/48 wells |
E0125MK | Monkey | Monkey Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit | 96 wells/48 wells |
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E0127MK | Monkey | Monkey Macrophage Migration Inhibitory Factor,MIF ELISA Kit | 96 wells/48 wells |
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E0133MK | Monkey | Monkey secreted frizzled-related protein 1,SFRP1 ELISA kit | 96 wells/48 wells |
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E0136MK | Monkey | Monkey Tissue factor pathway inhibitor,TFPI ELISA kit | 96 wells/48 wells |
E0137MK | Monkey | Monkey Toll-like receptor 2,TLR2 ELISA kit | 96 wells/48 wells |
E0138MK | Monkey | Monkey Vascular Endothelial cell Growth Factor,VEGF ELISA Kit | 96 wells/48 wells |
E0139MK | Monkey | Monkey Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit | 96 wells/48 wells |
E0140MK | Monkey | Monkey Stromal cell derived factor 1,SDF-1 ELISA Kit | 96 wells/48 wells |
E0141MK | Monkey | Monkey Nuclear Factor Kappa B,NFkB ELISA Kit | 96 wells/48 wells |
E0142MK | Monkey | Monkey Interleukin 12,IL-12 ELISA Kit | 96 wells/48 wells |
E1374MK | Monkey | Monkey Carboxymethyl Lysine,CML ELISA kit | 96 wells/48 wells |