【Protein A-HRP】Southernbiotech Protein A-HRP
蛋白A(Protein A)-HRP
蛋白A(Protein A)是最初在細菌金黃色葡萄球菌(bacterium Staphylococcus aureus)的細胞壁中發現的42kD表面蛋白 (surface protein)。 由於其結合免疫球蛋白(immunoglobulins)的能力,它被廣泛用於生物化學研究(biochemical research)和工業(industry)。 它由五個同源的Ig結合結構域(homologous Ig-binding domains)組成,折疊成三螺旋束(three-helix bundle)。 每個結構域能夠結合來自許多哺乳動物物種的蛋白質,最值得注意的是IgG(immunoglobulins)。 它結合大多數免疫球蛋白的Fc區內的重鏈(heavy chain)。將蛋白A標誌HRP (貨號7300-05 ),可應用於檢測一抗,例如ELISA,ELISPOT,IHC或Western印跡 (Western blotting) 。 IP隨後進行Western blotting應用,HRP蛋白A將避免IgG重鏈/輕鏈(heavy/light chain)問題,因為它僅識別其天然形式的IgG,並且用於IP靶標的抗體通常與免疫沉澱物一起煮沸並變性。
產品介紹: Protein A-HRP,具HRP標誌。針對Binds the Fc portion of human IgG1, IgG2, and IgG4 as well as mouse IgG2aand IgG2b; also binds rabbit, porcine, guinea pig, and canine IgG具特異性。來源為Staphylococcus aureus。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。結構Monomeric protein lacking cysteine residues; Mr 42 kDa
Source | Staphylococcus aureus |
Specificity | Binds the Fc portion of human IgG1, IgG2, and IgG4 as well as mouse IgG2aand IgG2b; also binds rabbit, porcine, guinea pig, and canine IgG |
Buffer Formulation | 50% Glycerol/50% Phosphate buffered saline, pH 7.4 |
Volume | 1.0 mL |
Storage & Handling | 2-8°C Please refer to product specific SDS |
Applications | ELISA – Quality tested 1-7 Purification WB 8 IP |
Recommended Dilutions | Please refer to product specific Technical Bulletin |
References
- Sondgeroth K, Blitvich B, Blair C, Terwee J, Junge R, Sauther M, et al. Assessing flavivirus, lentivirus, and herpesvirus exposure in free-ranging ring-tailed lemurs in southwestern Madagascar. J Wildl Dis. 2007;43:40-7. (ELISA)
- Wycoff KL, Belle A, Deppe D, Schaefer L, Maclean JM, Haase S, et al. Recombinant anthrax toxin receptor-Fc fusion proteins produced in plants protect rabbits against inhalational anthrax. Antimicrob Agents Chemother. 2011;55:132-9. (ELISA)
- Duthie MS, Truman RW, Goto W, O’Donnell J, Hay MN, Spencer JS, et al. Insight toward early diagnosis of leprosy through analysis of the developing antibody responses of Mycobacterium leprae-infected armadillos. Clin Vaccine Immunol. 2011;18:254-9. (ELISA)
- Arzola L, Chen J, Rattanaporn K, Maclean JM, McDonald KA. Transient co-expression of post-transcriptional gene silencing suppressors for increased in planta expression of a recombinant anthrax receptor fusion protein. Int J Mol Sci. 2011;12:4975-90. (ELISA)
- Karuppanan K, Duhra-Gill S, Kailemia MJ, Phu ML, Lebrilla CB, Dandekar AM, et al. Expression, purification, and biophysical characterization of a secreted anthrax decoy fusion protein in Nicotiana benthamiana. Int J Mol Sci. 2017;18:89. (ELISA)
- Xiong Y, Li Q, Kailemia MJ, Lebrilla CB, Nandi S, McDonald KA. Glycoform modification of secreted recombinant glycoproteins through kifunensine addition during transient vacuum agroinfiltration. Int J Mol Sci. 2018;19:890. (ELISA)
- Sukenik SC, Karuppanan K, Li Q, Lebrilla CB, Nandi S, McDonald KA. Transient recombinant protein production in glycoengineered Nicotiana benthamiana cell suspension culture. Int J Mol Sci. 2018;19:1205. (ELISA)
- Naskar P, Puri N. Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis. Biol Open. 2017;6:1257-69. (WB)
SBA熱銷商品 7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 | DAPI螢光封片劑 | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I | 一級抗體