【橫紋肌溶解症檢測】肌球蛋白/CK肌酸激酶/CPK肌酸磷酸激酶檢測分析

「橫紋肌溶解症」是指骨骼肌(橫紋肌)產生急速損傷，導致肌肉細胞壞死及細胞膜破壞，肌肉一些蛋白質以及肌球蛋白(myoglobin) 便會滲漏出來，進入血液中並隨後出現在尿中。尿中出現肌球蛋白的狀況稱為「肌球蛋白尿」。所以「橫紋肌溶解症」其實只是一種症狀，有許多的原因都會造成此一現象。當肌球蛋白出現在尿中時，尿的顏色會呈現深棕色，由於肌球蛋白的構造與血紅素有些類似，所以用一般測血尿的試紙來測試也會出現陽性反應。如果血中的肌球蛋白不多，幾天內通常都會痊癒。如果肌肉破壞的比較厲害，尿中肌球蛋白的濃度太高時，就會導致腎臟機能的損傷，甚至導致急性腎衰竭。當肌肉遭受損傷時，肌肉中的酵素也會釋放到血中來，其中最常運用的檢查就是肌氨酸激脢 (creatine kinase， CK)值的測定，在正常情況下CK值應低於200 U/L，在產生「橫紋肌溶解症」時，CK值會高達數千或甚至上萬。資料來源請按此

肌紅蛋白ELISA檢測 | Myoglobin ELISA kit 貨號E4326-100

A Sandwich ELISA kit for the quantitative measurement of human myoglobin in serum, plasma, culture supernatants and other biological fluids.

產品描述：用作氧氣的儲備供應並促進肌肉內氧氣的運動。肌紅蛋白是肌肉組織的主要攜氧色素。肌肉細胞中高濃度的肌紅蛋白使生物體能夠長時間屏住呼吸。 BioVision的Myoglobin ELISA試劑盒是一種夾心ELISA檢測，用於定量測量血清，血漿和細胞培養上清液中的人肌紅蛋白。顏色密度與從樣品中捕獲的人肌紅蛋白的量成比例。Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Myoglobin is the primary oxygen-carrying pigment of muscle tissues. High concentrations of myoglobin in muscle cells allow organisms to hold their breath for a longer period of time. BioVision’s Myoglobin ELISA kit is a sandwich ELISA assay for the quantitative measurement of human Myoglobin in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human Myoglobin captured from the samples.

Cat # +Size	E4326-100
Size	100 assays
Kit Summary	• Detection method- Absorbance (450 nm) • Species reactivity- human • Application- quantitative measurement of human total myoglobin in serum, plasma, tissue homogenates and other biological fluids.
Detection Method	Absorbance (450 nm)
Species Reactivity	Human
Applications	This ELISA kit is used for quantitative measurement of total myoglobin in human serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits	• Easy, convenient and time-saving method to measure the level of myoglobin in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids. • Detection Range: 1.563 – 100 ng/ml • Sensitivity: < 0.938 ng/ml • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Kit Components	• Micro ELISA Plate • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

肌酸激酶活性比色分析試劑盒 | 貨號K777-100

Creatine Kinase (CK) Activity Colorimetric Assay Kit

產品描述：肌酸激酶（Creatine Kinase ,CK）也稱為肌酸磷酸激酶（creatine phosphokinase , CPK）和ATP：肌酸N-磷酸轉移酶(creatine N-phosphotransferase)是常見的細胞酶（EC 2.7.3.2）。它催化肌酸(creatine)和ATP可逆轉化為ADP和磷酸肌酸(phosphocreatine)。 CK在各種組織和細胞類型中廣泛表達，在橫紋肌(striated muscles)，心臟組織(heart tissue)和腦(brain)中具有最高活性。 CK由兩個亞基組成：M（(muscle)肌肉）和B（(brain)腦），並且具有三種同工酶：CK-MM（(skeleton muscle)骨骼肌），CK-MB（(cardiac muscle)心肌）和CK-BB（CK-BB (brain)腦）。 CK水平升高與許多疾病相關，例如心肌梗塞(myocardial infarction)，肌營養不良(muscular dystrophy)，肺梗塞(pulmonary infarction)和腦腫瘤(brain tumors)。準確測量CK對早期診斷，預測和治療策略至關重要。在BioVision的肌酸激酶活性比色分析試劑盒中，肌酸激酶(creatine kinase )將肌酸(creatine)轉化為磷酸肌酸(phosphocreatine)和ADP。產生的磷酸肌酸(phosphocreatine)和ADP與CK酶混合物反應形成中間體，其將無色探針還原成有色產物，在450nm處具有強吸光度。 CK活性測定具有高通量適應性，簡單靈敏。該檢測試劑盒可檢測小於1 mU的肌酸激酶活性。

Cat # +Size	K777-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Applications	The kit can detect creatine kinase activity less than 1 mU.
Features & Benefits	• Simple procedure; takes ~ less than 40 minutes • Fast and convenient
Kit Components	• CK Assay Buffer • CK Substrate • ATP (Lyophilized) • CK Enzyme Mix (Lyophilized) • CK Developer (Lyophilized) • NADH Standard (Lyophilized) • Positive Control (Lyophilized)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.

丙氨酸氨基轉移酶（ALT或SGPT）活性比色/螢光測定試劑盒 | 貨號K752-100

Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit

產品描述：丙氨酸轉氨酶（ALT）是一種轉氨酶（EC 2.6.1.2）。它也被稱為血清穀丙轉氨酶（SGPT）或丙氨酸轉氨酶（ALAT）。 ALT存在於血清和各種身體組織中，但通常與肝臟有關。它催化反應：α-酮戊二酸+丙氨酸⇌谷氨酸+丙酮酸它通常在臨床上作為診斷肝功能測試的一部分來測定以確定肝臟健康。診斷上，幾乎總是以單位/升（U / L）來衡量。在BioVision的ALT檢測試劑盒中，ALT催化氨基從丙氨酸到ket-酮戊二酸的轉移，該可逆轉氨反應的產物是丙酮酸和谷氨酸。在將幾乎無色的探針同時轉換成顏色（λmax= 570nm）和螢光（Ex / Em = 535 / 587nm）的反應中檢測到丙酮酸鹽。該試劑盒提供了一種快速，簡單，靈敏和可靠的檢測方法，適用於ALT的高通量活性檢測，檢測限為每孔10 mU。

Cat # +Size	K752-100
Size	100 assays
Detection Method	Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Mammalian
Applications	Evaluation of pentose phosphate pathway
Features & Benefits	• Simple procedure • Fast and convenient • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit Components	• ALT Assay Buffer • OxiRed Probe (in DMSO) • ALT Enzyme Mix • ALT Substrate • Pyruvate Standard (100 nmol/µl) • ALT positive control

白蛋白（BCG）檢測 | （AST）活性測定試劑 | ALT/SGPT）活性比色螢光測定試劑 | Ammonia測定試劑 | 膽紅素分析試劑盒

引用文獻

Takasu, C. et al., (2017) Treatment with dimethyl fumarate ameliorates liver ischemia/reperfusion injury, World J Gastroenterol, Oct.2017, 23(25): 4508–4516.

Zhang, J. et al., (2017) Jia-Jian-Di-Huang-Yin-Zi decoction reduces apoptosis induced by both mitochondrial and endoplasmic reticulum caspase12 pathways in the mouse model of Parkinson’s disease , ournal of Ethnopharmacology, May 2017, 203: 69-79

Mohamed Aboul Ezz et al., (2017) The effect of cholesterol loaded cyclodextrins on post-thawing quality of buffalo semen in relation to sperm DNA damage and ultrastructure, Reproductive Biology, 2017, 17: 42–50

Myungsuk Kim et al., (2017) Chicoric acid attenuate a nonalcoholic steatohepatitis by inhibiting key regulators of lipid metabolism, fibrosis, oxidation, and inflammation in mice with methionine and choline deficiency, Mol Nutr Food Res, 2017, 61(5)

Cho, Haaglim et al. (2017) ENOblock, a unique small molecule inhibitor of the non-glycolytic functions of enolase, alleviates the symptoms of type 2 diabetes, Sci Rep. 2017 Mar 8;7:44186.

鈣呈色測定試劑盒 | 貨號K380 Calcium Colorimetric Assay Kit

產品介紹: Calcium 鈣是所有生物體中必不可少的，Ca2 +螯合和釋放出細胞質作為許多細胞過程的信號。在 bones骨骼和teeth 牙齒中發現99％的鈣，其餘1％存在於blood 血液和 soft tissue軟組織中。Serum calcium 血清鈣水平受到嚴格控制（8.4-11.4 mg / dL），超出該範圍的任何變化都會產生嚴重影響。鈣在調節血管的 constriction 收縮和relaxation 鬆弛，nerve impulse transmission神經衝動傳遞， muscle contraction肌肉收縮和激素分泌方面發揮作用。鈣離子通道控制鈣離子跨越細胞膜的遷移，允許多種酶的活化和抑制。低鈣水平的原因包括chronic kidney failure慢性腎功能衰竭， vitamin D deficiency維生素D缺乏症，以及嚴重酒精中毒可能發生的低血鎂水平。BioVision的鈣測定試劑盒利用在calcium ions鈣離子和 0-cresolphthalein O-甲酚酞之間形成的chromogenic complex顯色複合物，於575nm測量吸收（λ= 575nm），在鈣濃度0.4-100mg / dL（0.1-25mM）的生理學重要範圍內提供簡單的測定方法。

Cat # +Size	K380-250
Size	250 assays
Detection Method	Absorbance (575 nm)
Species Reactivity	Mammalian
Applications	The kit can detect calcium concentration 0.4-100 mg/dL (0.1-25 mM).
Features & Benefits	• Simple procedure; takes less than 20 minutes • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable
Kit Components	• Calcium Assay Buffer • Chromogenic Reagent • Calcium Standard (500 mM)

注意事項: 肝素是唯一不會干擾的抗凝血劑。樣品製備：血清或尿液樣品可直接用於該測定。放置10μl樣品在96孔板中的孔中。對於其他液體樣品，添加2-50μl樣品
個人好。用dH2O使總體積達到50μl。可以測定樣品沒有任何事先處理。一些MRI造影劑可能會導致瞬態干擾化驗

其它商品 α-Galactosylceramide | α-Tocopheryl succinate | Wogonin | VK3-OCH3 | Vinorelbine Tartrate

Jiao Jiao Zhang, et al., (2018) MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation, Scientific Reports, Jun. 2018, 29884805

Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.

Wei Wei et al. (2017) In vitro osteogenic induction of bone marrow mesenchymal stem cells with a decellularized matrix derived from human adipose stem cells and in vivo implantation for bone regeneration, J. Mater. Chem. B, 2017,5, 2468-2482

Chang et al., Effect of ED-71, an analogue of Vitamin D3, on intestinal neoplasia in the Apc+/Min-FCCC mouse model. Cancer Res., Aug 2015; 75: 2806.

Krieger et al., Effect of Potassium Citrate on Calcium Phosphate Stones in a Model of Hypercalciuria. J. Am. Soc. Nephrol., Apr 2015; 10.1681/ASN.2014121223.

鈣呈色測定 | Magnesium鎂呈色測定 |磷酸鹽比色測定 | 鈉呈色分析 | Zinc鋅呈色測定

肌酸酐測定試劑盒 |貨號K625-100

Creatinine Colorimetric/Fluorometric Assay Kit

原理介紹: 肌酸酐是肌酸磷酸酯的分解產物。產生肌酸酐並以恆定的速率排出，血肌酐用於測定腎功能的腎小球濾過率（GFR）。血液肌酐水平只有在對腎單位有顯著（> 75％）的傷害的情況下才會增加。經常使用肌酸酐清除作為標準化其他化合物如異前列烷烷的排泄的手段。 BioVision的肌酸酐測定試劑盒可以準確，方便地測量生物液體如血清，尿液或腦脊液中的肌酸濃度。在測定中，通過肌酸酐酶將肌酸酐轉化為肌酸，將肌酸轉化為肌氨酸，其被特異性氧化以產生與探針反應以產生紅色（λmax= 570nm）和螢光（Ex / Em = 538 / 587nm）。與苦味酸測定不同，該試劑盒適用於血清/血漿肌酐測定，以及尿液和其他生物樣品。

Cat # +Size	K625-100
Size	100 assays
Detection Method	Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Mammalian
Applications	N/A
Features & Benefits	• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Creatinine
Kit Components	• Creatinine Assay Buffer • Creatinine Probe • Creatinase • Creatininase • Creatinine Enzyme Mix • Creatinine (10 µmol)

引用文獻

Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.

Zhang, Lu et al. (2017) Nerolidol Protects Against LPS-induced Acute Kidney Injury via Inhibiting TLR4/NF-κB Signaling, Phytother Res. 2017 Mar;31(3):459-465.

Yao et al., Evaluation of urine fibrinogen level in a murine model of contrast-induced nephropathy. Vascular, Jun 2015; 10.1177/1708538115593039.

Burris et al., Estrogen directly and specifically downregulates NaPi-IIa through the activation of both estrogen receptor isoforms (ERα and ERβ) in rat kidney proximal tubule. Am J Physiol Renal Physiol, Mar 2015; 308: F522 – F534.

Wang et al., Astragaloside IV inhibits renal tubulointerstitial fibrosis by blocking TGF-β/Smad signaling pathway in vivo and in vitro.Experimental Biology and Medicine, Oct 2014; 239: 1310 – 1324.

天冬氨酸氨基轉移酶（AST）測定試劑盒–乙醛酸循環分析商品 | K753 -100

Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit

乙醛酸循環是三羧酸循環的變化，是在植物，細菌，原生生物和真菌中發生的合成代謝途徑。當不能獲得諸如葡萄糖等複雜來源時，該循環允許細胞利用簡單的碳化合物作為碳源。 BioVision為涉及乙醛酸途徑的不同代謝物提供易於使用和高通量的適應性測定試劑盒。這些測定試劑盒是高度敏感的並且與廣泛的樣品類型兼容

產品介紹: 天冬氨酸氨基轉移酶（AST）也稱為谷氨酸 – 草醯乙酸轉氨酶（GOT）是與更特異性的丙氨酸轉氨酶（ALT）相似的轉氨酶（EC 2.6.1.1）。雖然臨床上通常包括作為診斷性肝功能檢查的一部分，但AST具有更廣泛的臨床實用性，因為其也可能在影響其他器官的疾病中升高，例如心肌梗塞中的心臟或肌肉，還有急性胰腺炎，急性溶血性貧血，嚴重燒傷，急性腎臟疾病，肌肉骨骼疾病和創傷。它催化反應：天冬氨酸+⍺-酮戊二酸⇌草酰乙酸鹽+谷氨酸鹽診斷性地，幾乎總是以單位/升（U / L）測量。在BioVision的AST測定試劑盒中，氨基被從天冬氨酸轉移到⍺-酮戊二酸。這種可逆轉氨基反應的產物是草醯乙酸和谷氨酸。在將近似無色探針同時轉化為顏色（λmax= 450nm）的反應中檢測到谷氨酸。該試劑盒提供快速，簡單，靈敏和可靠的測試，適合作為AST的高通量活性測定，檢測限為10 mU /孔。。

Cat # +Size	K753-100
Size	100 assays
Detection Method	Absorbance (450 nm)
Species Reactivity	Mammalian
Applications	This assay detects AST/ SGOT with a detection limit of 10 mU per well.
Features & Benefits	• Simple procedure • Fast, reliable and convenient • Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit Components	• AST Assay Buffer • AST Enzyme Mix • Developer • AST Substrate • Glutamate Standard (0.1M) • AST Positive Control

引用文獻

Mahesh, Malleswarapu et al. (2016) Carrot Juice Administration Decreases Liver Stearoyl-CoA Desaturase 1 and Improves Docosahexaenoic Acid Levels, but Not Steatosis in High Fructose Diet-Fed Weanling Wistar Rats., Prev Nutr Food Sci. 2016 Sep;21(3):171-180.

Murugesan et al., Kinin B1 Receptor Inhibition With BI113823 Reduces Inflammatory Response, Mitigates Organ Injury, and Improves Survival Among Rats With Severe Sepsis. The Journal of Infectious Disease, Sep 2015; 10.1093/infdis/jiv426.

Lorentz et al., Engineered binding to erythrocytes induces immunological tolerance to E. coli asparaginase.SciAdv, Jul 2015; 1: e1500112.