亞硝酸鹽檢測試劑盒 (Griess Reagent) | 貨號k544-200  亞硝酸鹽（Nitrite; NO2 – ）累積量測定套組-Griess Reagent

在幾乎每個生物體中都發現了Nitrogen-based ions氮基離子，Nitrogen-based ions亞硝酸鹽和nitrate硝酸鹽。此外，在哺乳動物中發現內源亞硝酸鹽水平，也可從dietary sources膳食來源獲得。在人類中，nitrit亞硝酸鹽 (nitrite) 進一步代謝為一氧化氮(Nitric Oxide)和其他活性氮物質（reactive nitrogen species, nitrogen oxides氮氧化物）。硝酸鹽 – 亞硝酸鹽 –  NO生化途徑因其參與細胞信號傳導(cell signaling)，缺氧依賴性反應(hypoxia-dependent response )和血流調節而眾所周知。最近的研究表明線粒體中亞硝酸鹽還原為氮氧(Nitrogen Oxygen )。具體而言，肌紅蛋白(myoglobin)和黃嘌呤氧化還原酶(xanthine oxidoreductase)可在低氧條件下產生NO，從而導致線粒體呼吸。BioVision的亞硝酸鹽檢測試劑盒使用Griess Reagent，這是一種用於估算亞硝酸鹽的常用測量方式。在該測定中，使用Griess試劑I將亞硝酸鹽還原為氮氧化物。然後，氮氧化物與Griess Reagent II反應形成穩定的產物，其可通過其在OD 540nm處的吸光度來檢測。一個步驟快速簡單分析，檢測低至1 nmol /孔的亞硝酸鹽水平。

ELISA儀器設備請按此  (標配405、450、492、630 nm濾片/最高可擴充至7濾片）（k544 λ= 540 nm）

Kit Summary	• Detection method- Absorbance (540 nm) • Species reactivity- Mammalian • Applications- BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
Detection Method	Detection method- Absorbance (540 nm)
Species Reactivity	Eukaryotes
Applications	Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
Features & Benefits	• Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite.
Kit Components	• Assay Buffer • Griess Reagent R1 • Griess Reagent R2 • Nitrite Standard

貨號	試劑品項	操作手冊下載
K816	螢光蛋白(fluorescent proteins)套組 | 貨號K816-6-100
K658	醋酸比色分析試劑盒 | 貨號K658-100
1056	Propidium Iodide 死細胞核酸染劑 | 貨號1056
K742	Tyrosinase酪氨酸酶活性測定試劑盒（呈色法） | 貨號K742-100
K1463	Coronavirus SARS-CoV-2 IgM / IgG抗體檢測卡/快篩試劑 貨號K1463
K787	乙醇脫氫酶測定試劑盒  | 貨號K787-100
K731	醛脫氫酶活性比色測定試劑盒 | 貨號K731-100
2589	Hygromycin B  | 貨號2589
K761	Glutathione Reductase Activity Colorimetric Assay Kit呈色分析套組 |貨號K761-200
K762	GPx呈色分析套組Glutathione Peroxidase Activity Colorimetric Assay Kit
K952	TG甘油三酯定量試劑盒 | Triglyceride Quantification Colorimetric Assay Kit (384-well) |  貨號K952
K629	Glutamate麩胺酸檢測 | 貨號K629-100
7903	D-Luciferin Potassium Salt  D-螢光素鉀鹽 | 貨號7903-1G
1557	抗生素系列產品  | G418 sulfate | G418抗生素 | Antibiotic G418 1557
2829	Taurocholic acid sodium salt牛磺酸鈉 | 貨號2829
K568	焦磷酸鹽（PPi）分析試劑盒 | 貨號K568-100
1215	(TMB) 3,3’5,5′-四甲基聯苯胺| 貨號1215-100
K4219	Dopamine多巴胺檢測 （酵素免疫分析） | 貨號K4219
K544	亞硝酸鹽檢測試劑盒 (Griess Reagent) | 貨號k544-200