【Genedirex預染蛋白質標準液】 BlueRAY Prestained Protein Ladder 貨號PM006-0500
預染蛋白質Marker 500ul | BlueRAY Prestained Protein Ladder |貨號: PM006-0500
Prestain protein marker, 10 to 180 kDa
Genedirex專攻預染蛋白Marker、DNA Marker等相關產品。Genedirex Prestained Protein Ladder是一種擁有三種不同顏色及十種不同分子量之蛋白質標識。其所能標示之分子量範圍從10到180 kDa,其中含有75 kDa的紅色條紋標識及25 kDa的綠色條紋標識。本產品乃是專門設計用於監測蛋白質電泳中蛋白質分離之過程及評估進行西方點墨法後,蛋白質轉移至轉漬膜 (PVDF, nylon, or nitrocellulose)之效率和對所偵測蛋白質之分子量做最後評估時所用。本產品使用時相當方便,無須任何處理及加熱步驟即可立即使用。
Prestain protein marker產品使用
本產品中之十種不同蛋白質之濃度皆介於0.2~ 0.4 m g/ml 之間,且溶於含有20Mm Tris-phosphate (pH 7.5), 2% SDS, 1mM 2-Mercaptoethanol, 3.6M urea, and 15% glycerol之溶液中。
Quality Control: 本產品出品前接經由完整之SDS- polyacrylamide 電泳及西方點墨法測試。
Storage:本產品可穩定儲存於4 0C 中 3個月或是於 -200C 24個月。
Prestain protein marker產品特點
•本產品標識條紋清晰且分子量穩定。
•本產品中含有兩條彩色條紋標識-分別是75 kDa的紅色條紋及25 kDa的綠色條紋。
•不需煮沸可立即使用。
•使用15-well or 10-well mini-gel 進行電泳時,每次使用3 μl or 5 μl 即可得到清晰的實驗結果。
•進行西方點墨法時,每次使用2-3μl即可得到清晰的實驗結果。
The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Trisglycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
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