【透析產品需知】常用之透析物質孔徑尺寸對照表
透析為利用分子會從高濃度擴散到低濃度,將待透析液放在具分子大小選擇性的透析膜中,進行物質交換。此時 大分子的溶質會被保留,而小分子溶質會通過透析膜移除,最終達到去除的效果。一般而言,常規的透析需要進行 2天的時間,才能達到透析的目的。而擴散係數(diffusion coefficients)、pH、溫度、時間、物質濃度、溶液 體積、緩衝液體積,緩衝液更換次數、透析膜表面積、透析膜厚度,攪拌等因素都會影響到透析的效率。
透析應用: 大分子淨化、蛋白濃縮、溶質分餾、去除雜質、改變pH值、脫鹽、溶液置換、親和研究、電洗脫。
一般常用之透析物質之孔徑尺寸表:
選擇正確的截留分子量(MWCO)
由於透析膜由海綿狀交聯聚合物組成,代表膜孔徑的截留分子量(MWCO)是一個間接衡量膜分離性能的參數。更準確的說,膜的截留分子量可定義為截留率至為少90%的溶質的分子量。由於溶質的滲透特性取決於分子形狀、水合化程度、離子電荷和極性,我們建議選擇截留分子量是被截留物質的分子量的一半/或是將要透過的物質的最大分子量的兩倍
膜等級新選擇 | 超純度Biotech透析膜 (生物技術級)
Biotech”超純”等級透析膜;無重金屬污染物與無硫化物的殘留,品質第一,使用前不需要再清洗或預處理,DI清水沖洗後即可使用。Biotech提供CE及RC膜兩種生物技術等級之透析膜選擇。CE膜之最小分子量0.1-0.5kD,分子量選擇更廣及多樣性,用於分子量之cut off設計;RC膜之化學耐受性佳,可提供化學溶液之透析。Spectrum提供2種不同的生物技術級膜”Biotech類型,以滿足實驗室透析應用的不同需求。
CE膜或RC膜選擇 | 請先參閱 Spectrum透析膜與化學溶液相溶性比較表
透析膜等級BIOTECH | 膜材質CE膜 | 分子量0.1-100KD | 物理性質不透明、較無彈性、疏水性 |有機溶劑耐受度為一般 | pH範圍2 – 9可承受溫度上限37°C |建議之透析夾Universal透析夾。Biologically inert & ultra-pure, Biotech Cellulose Ester (CE) Membrane offers the largest range of concise MWCO’s (100 – 1,000,000 Daltons) for desalting, isolating ionic species and macromolecular purifications.
NEW! Biotech CE Dialysis Trial Kits (1 meter/kit) Biotech CE Dialysis Tubing (10 meter/roll)
MWCO | FW: | 10 mm | 16 mm | 24 mm | 31 mm | |
(kD) | Dia: | 6.4 mm | 10 mm | 15 mm | 20 mm | |
Vol/L: | 0.32 ml/cm | 0.79 ml/cm | 1.8 ml/cm | 3.1 ml/cm | ||
131048 | 131054 | 131057 | 131060 | |||
131084 | 131090 | 131093 | 131096 | |||
0.1 – 0.5 | 131192 | 131198 | 131201 | 131204 | ||
0.5 – 1.0 | 131264 | 131270 | 131273 | 131276 | ||
3.5 – 5 | 131336 | 131342 | 131345 | 131348 | ||
8 – 10 | 131372 | 131378 | 131381 | 131384 | ||
20 | 131408 | 131414 | 131417 | 131420 | ||
50 | 131450 | 131456 | ||||
100 | 131486 | 131492 |
透析膜等級BIOTECH | 膜材質RC | 分子量3.5-20kD | 物理性質不透明有彈性、疏水性 | 有機溶劑耐受度佳 | pH範圍2–12可承受溫度上限60°C | 建議之透析夾Universal透析夾。Biotech RC membrane can tolerate concentrated-weak acids & bases, dilute-strong acids & bases, most alcohols and some mild or dilute organic solvents. Exposure to strong polar or organic solvents may damage RC membranes. Use with pH 2-12 & temp 4-60 °C.
NEW! Biotech RC Dialysis Trial Kit (0.5 meter/kit) Biotech RC Dialysis Tubing (5 meter/roll)
MWCO (kD) | FW: | 10 mm | 16 mm |
Dia: | 6.4 mm | 10 mm | |
Vol/L: | 0.32 ml/cm | 0.79 ml/cm | |
3.5 – 5 | 133192 | 133198 | |
8 – 10 | 133264 | 133270 | |
20 | 133336 | 13334 |
透析膜等級Standard | 膜材質RC | 分子量1-25kD | 物理性質透明有彈性、疏水性 | 有機溶劑耐受度佳 | pH範圍2–12可承受溫度上限121°C | 建議之透析夾Universal透析夾或Spectra/Por®透析夾。Standard Grade RC can be used with dilute strong acids and bases, concentrated weak acids and bases, most alcohols and some mild or dilute organics, including DMSO. Standard RC can tolerate pH 2 – 12 and temperatures 4-121 °C
MWCO | Flat Width (mm) |
Diameter (mm) |
Volume/Length (ml/cm) |
Part No. |
1 kD | 18 | 11.5 | 1.1 | 132636 |
38 | 24 | 4.6 | 132638 | |
45 | 29 | 6.4 | 132640 | |
2 kD | 18 | 11.5 | 1.1 | 132620 |
38 | 24 | 4.6 | 132625 | |
45 | 29 | 6.4 | 132633 | |
3.5 kD | 18 | 11.5 | 1.1 | 132590 |
45 | 29 | 6.4 | 132592 | |
54 | 34 | 9.3 | 132594 | |
8 kD | ||||
12 | 7.5 | 0.45 | 132579 | |
18 | 11.5 | 1.1 | 128058 | |
24 | 15 | 1.8 | 132580 | |
32 | 20.4 | 3.3 | 132582 | |
40 | 25.5 | 5.1 | 132584 | |
50 | 32 | 7.9 | 132586 | |
10 kD | ||||
12 | 7.5 | 0.45 | 132570 | |
18 | 11.5 | 1.1 | 128118 | |
24 | 15 | 1.8 | 132572 | |
32 | 20.4 | 3.3 | 132574 | |
45 | 29 | 6.4 | 132576 | |
15 kD | ||||
12 | 7.5 | 0.45 | 132560 | |
18 | 11.5 | 1.1 | 128158 | |
24 | 15 | 1.8 | 132562 | |
32 | 20.4 | 3.3 | 132564 | |
45 | 29 | 6.4 | 132566 | |
25 kD | ||||
12 | 7.5 | 0.45 | 132550 | |
18 | 11.5 | 1.1 | 128218 | |
28 | 18 | 2.5 | 132552 | |
34 | 22 | 3.7 | 132554 | |
50 kD | 10 | 6.4 | 0.32 | 132539 |
12 | 7.5 | 0.45 | 132540 | |
28 | 18 | 2.5 | 132542 | |
34 | 22 | 3.7 | 132544 |